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Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis
The main diagnostic indicators of ovarian cancer (OC), including carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4), show good sensitivity and poor specificity or vice versa. This study investigated changes in CA125 and HE4 expression and their correlation in serum-derived exosome...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532513/ https://www.ncbi.nlm.nih.gov/pubmed/33033528 http://dx.doi.org/10.7150/jca.48531 |
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author | Chen, Zhixiang Liang, Qianxin Zeng, Hua Zhao, Qing Guo, Zhaodi Zhong, Rihui Xie, Manlin Cai, Xiuping Su, Jing He, Zhiliang Zheng, Lei Zhao, Kewei |
author_facet | Chen, Zhixiang Liang, Qianxin Zeng, Hua Zhao, Qing Guo, Zhaodi Zhong, Rihui Xie, Manlin Cai, Xiuping Su, Jing He, Zhiliang Zheng, Lei Zhao, Kewei |
author_sort | Chen, Zhixiang |
collection | PubMed |
description | The main diagnostic indicators of ovarian cancer (OC), including carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4), show good sensitivity and poor specificity or vice versa. This study investigated changes in CA125 and HE4 expression and their correlation in serum-derived exosomes of 55 patients with OC (OC group), 33 patients with malignant tumors (non-OC group), and 55 normal controls (NC group). We compared serum and exosomal CA125 and HE4 levels to determine whether their contents in exosomes were elevated. We also compared the diagnostic efficacy of serum HE4, serum CA125, exosomal CA125, and serum HE4+exosomal CA125 in OC using the receiver operating characteristic (ROC) curve. CA125 levels in serum-derived exosomes in all groups significantly increased (P < 0.0001) compared with serum CA125 levels. HE4 was undetected in exosomes. The ROC curve showed the following values: serum CA125: 0.9093 (area), 87.27% (sensitivity), and 90.91% (specificity); serum HE4: 0.9302, 83.64%, and 94.55%; exosomal CA125: 0.9755, 94.55%, and 92.73%; and serum HE4+exosomal CA125: 0.9861, 96.36%, and 92.73%. In conclusion, CA125 can be detected at higher levels in exosomes than in serum, significantly improving OC diagnosis sensitivity. The serum HE4+exosomal CA125 combination significantly improves OC diagnostic efficiency. |
format | Online Article Text |
id | pubmed-7532513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-75325132020-10-07 Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis Chen, Zhixiang Liang, Qianxin Zeng, Hua Zhao, Qing Guo, Zhaodi Zhong, Rihui Xie, Manlin Cai, Xiuping Su, Jing He, Zhiliang Zheng, Lei Zhao, Kewei J Cancer Research Paper The main diagnostic indicators of ovarian cancer (OC), including carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4), show good sensitivity and poor specificity or vice versa. This study investigated changes in CA125 and HE4 expression and their correlation in serum-derived exosomes of 55 patients with OC (OC group), 33 patients with malignant tumors (non-OC group), and 55 normal controls (NC group). We compared serum and exosomal CA125 and HE4 levels to determine whether their contents in exosomes were elevated. We also compared the diagnostic efficacy of serum HE4, serum CA125, exosomal CA125, and serum HE4+exosomal CA125 in OC using the receiver operating characteristic (ROC) curve. CA125 levels in serum-derived exosomes in all groups significantly increased (P < 0.0001) compared with serum CA125 levels. HE4 was undetected in exosomes. The ROC curve showed the following values: serum CA125: 0.9093 (area), 87.27% (sensitivity), and 90.91% (specificity); serum HE4: 0.9302, 83.64%, and 94.55%; exosomal CA125: 0.9755, 94.55%, and 92.73%; and serum HE4+exosomal CA125: 0.9861, 96.36%, and 92.73%. In conclusion, CA125 can be detected at higher levels in exosomes than in serum, significantly improving OC diagnosis sensitivity. The serum HE4+exosomal CA125 combination significantly improves OC diagnostic efficiency. Ivyspring International Publisher 2020-09-14 /pmc/articles/PMC7532513/ /pubmed/33033528 http://dx.doi.org/10.7150/jca.48531 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Chen, Zhixiang Liang, Qianxin Zeng, Hua Zhao, Qing Guo, Zhaodi Zhong, Rihui Xie, Manlin Cai, Xiuping Su, Jing He, Zhiliang Zheng, Lei Zhao, Kewei Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis |
title | Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis |
title_full | Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis |
title_fullStr | Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis |
title_full_unstemmed | Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis |
title_short | Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis |
title_sort | exosomal ca125 as a promising biomarker for ovarian cancer diagnosis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532513/ https://www.ncbi.nlm.nih.gov/pubmed/33033528 http://dx.doi.org/10.7150/jca.48531 |
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