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Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis

The main diagnostic indicators of ovarian cancer (OC), including carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4), show good sensitivity and poor specificity or vice versa. This study investigated changes in CA125 and HE4 expression and their correlation in serum-derived exosome...

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Autores principales: Chen, Zhixiang, Liang, Qianxin, Zeng, Hua, Zhao, Qing, Guo, Zhaodi, Zhong, Rihui, Xie, Manlin, Cai, Xiuping, Su, Jing, He, Zhiliang, Zheng, Lei, Zhao, Kewei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532513/
https://www.ncbi.nlm.nih.gov/pubmed/33033528
http://dx.doi.org/10.7150/jca.48531
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author Chen, Zhixiang
Liang, Qianxin
Zeng, Hua
Zhao, Qing
Guo, Zhaodi
Zhong, Rihui
Xie, Manlin
Cai, Xiuping
Su, Jing
He, Zhiliang
Zheng, Lei
Zhao, Kewei
author_facet Chen, Zhixiang
Liang, Qianxin
Zeng, Hua
Zhao, Qing
Guo, Zhaodi
Zhong, Rihui
Xie, Manlin
Cai, Xiuping
Su, Jing
He, Zhiliang
Zheng, Lei
Zhao, Kewei
author_sort Chen, Zhixiang
collection PubMed
description The main diagnostic indicators of ovarian cancer (OC), including carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4), show good sensitivity and poor specificity or vice versa. This study investigated changes in CA125 and HE4 expression and their correlation in serum-derived exosomes of 55 patients with OC (OC group), 33 patients with malignant tumors (non-OC group), and 55 normal controls (NC group). We compared serum and exosomal CA125 and HE4 levels to determine whether their contents in exosomes were elevated. We also compared the diagnostic efficacy of serum HE4, serum CA125, exosomal CA125, and serum HE4+exosomal CA125 in OC using the receiver operating characteristic (ROC) curve. CA125 levels in serum-derived exosomes in all groups significantly increased (P < 0.0001) compared with serum CA125 levels. HE4 was undetected in exosomes. The ROC curve showed the following values: serum CA125: 0.9093 (area), 87.27% (sensitivity), and 90.91% (specificity); serum HE4: 0.9302, 83.64%, and 94.55%; exosomal CA125: 0.9755, 94.55%, and 92.73%; and serum HE4+exosomal CA125: 0.9861, 96.36%, and 92.73%. In conclusion, CA125 can be detected at higher levels in exosomes than in serum, significantly improving OC diagnosis sensitivity. The serum HE4+exosomal CA125 combination significantly improves OC diagnostic efficiency.
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spelling pubmed-75325132020-10-07 Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis Chen, Zhixiang Liang, Qianxin Zeng, Hua Zhao, Qing Guo, Zhaodi Zhong, Rihui Xie, Manlin Cai, Xiuping Su, Jing He, Zhiliang Zheng, Lei Zhao, Kewei J Cancer Research Paper The main diagnostic indicators of ovarian cancer (OC), including carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4), show good sensitivity and poor specificity or vice versa. This study investigated changes in CA125 and HE4 expression and their correlation in serum-derived exosomes of 55 patients with OC (OC group), 33 patients with malignant tumors (non-OC group), and 55 normal controls (NC group). We compared serum and exosomal CA125 and HE4 levels to determine whether their contents in exosomes were elevated. We also compared the diagnostic efficacy of serum HE4, serum CA125, exosomal CA125, and serum HE4+exosomal CA125 in OC using the receiver operating characteristic (ROC) curve. CA125 levels in serum-derived exosomes in all groups significantly increased (P < 0.0001) compared with serum CA125 levels. HE4 was undetected in exosomes. The ROC curve showed the following values: serum CA125: 0.9093 (area), 87.27% (sensitivity), and 90.91% (specificity); serum HE4: 0.9302, 83.64%, and 94.55%; exosomal CA125: 0.9755, 94.55%, and 92.73%; and serum HE4+exosomal CA125: 0.9861, 96.36%, and 92.73%. In conclusion, CA125 can be detected at higher levels in exosomes than in serum, significantly improving OC diagnosis sensitivity. The serum HE4+exosomal CA125 combination significantly improves OC diagnostic efficiency. Ivyspring International Publisher 2020-09-14 /pmc/articles/PMC7532513/ /pubmed/33033528 http://dx.doi.org/10.7150/jca.48531 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Chen, Zhixiang
Liang, Qianxin
Zeng, Hua
Zhao, Qing
Guo, Zhaodi
Zhong, Rihui
Xie, Manlin
Cai, Xiuping
Su, Jing
He, Zhiliang
Zheng, Lei
Zhao, Kewei
Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis
title Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis
title_full Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis
title_fullStr Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis
title_full_unstemmed Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis
title_short Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis
title_sort exosomal ca125 as a promising biomarker for ovarian cancer diagnosis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532513/
https://www.ncbi.nlm.nih.gov/pubmed/33033528
http://dx.doi.org/10.7150/jca.48531
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