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Magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing

We demonstrate a proof of concept for magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing via two photon polymerization (LDW via TPP) of a photopolymerizable superparamagnetic composite. The composite consisted of a commercially available, b...

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Autores principales: Paun, I. A., Mustaciosu, C. C., Mihailescu, M., Calin, B. S., Sandu, A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532536/
https://www.ncbi.nlm.nih.gov/pubmed/33009486
http://dx.doi.org/10.1038/s41598-020-73414-4
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author Paun, I. A.
Mustaciosu, C. C.
Mihailescu, M.
Calin, B. S.
Sandu, A. M.
author_facet Paun, I. A.
Mustaciosu, C. C.
Mihailescu, M.
Calin, B. S.
Sandu, A. M.
author_sort Paun, I. A.
collection PubMed
description We demonstrate a proof of concept for magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing via two photon polymerization (LDW via TPP) of a photopolymerizable superparamagnetic composite. The composite consisted of a commercially available, biocompatible photopolymer (Ormocore) mixed with 4 mg/mL superparamagnetic nanoparticles (MNPs). The micromagnets were designed in the shape of squares with 70 µm lateral dimension. To minimize the role of topographical cues on the cellular attachment, we fabricated 2D microarrays similar with a chessboard: the superparamagnetic micromagnets alternated with non-magnetic areas of identical shape and lateral size as the micromagnets, made from Ormocore by LDW via TPP. The height difference between the superparamagnetic and non-magnetic areas was of ~ 6 µm. In the absence of a static magnetic field, MNPs-free fibroblasts attached uniformly on the entire 2D microarray, with no preference for the superparamagnetic or non-magnetic areas. Under a static magnetic field of 1.3 T, the fibroblasts attached exclusively on the superparamagnetic micromagnets, resulting a precise 2D cell organization on the chessboard-like microarray. The described method has significant potential for fabricating biocompatible micromagnets with well-defined geometries for building skin grafts adapted for optimum tissue integration, starting from single cell manipulation up to the engineering of whole tissues.
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spelling pubmed-75325362020-10-06 Magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing Paun, I. A. Mustaciosu, C. C. Mihailescu, M. Calin, B. S. Sandu, A. M. Sci Rep Article We demonstrate a proof of concept for magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing via two photon polymerization (LDW via TPP) of a photopolymerizable superparamagnetic composite. The composite consisted of a commercially available, biocompatible photopolymer (Ormocore) mixed with 4 mg/mL superparamagnetic nanoparticles (MNPs). The micromagnets were designed in the shape of squares with 70 µm lateral dimension. To minimize the role of topographical cues on the cellular attachment, we fabricated 2D microarrays similar with a chessboard: the superparamagnetic micromagnets alternated with non-magnetic areas of identical shape and lateral size as the micromagnets, made from Ormocore by LDW via TPP. The height difference between the superparamagnetic and non-magnetic areas was of ~ 6 µm. In the absence of a static magnetic field, MNPs-free fibroblasts attached uniformly on the entire 2D microarray, with no preference for the superparamagnetic or non-magnetic areas. Under a static magnetic field of 1.3 T, the fibroblasts attached exclusively on the superparamagnetic micromagnets, resulting a precise 2D cell organization on the chessboard-like microarray. The described method has significant potential for fabricating biocompatible micromagnets with well-defined geometries for building skin grafts adapted for optimum tissue integration, starting from single cell manipulation up to the engineering of whole tissues. Nature Publishing Group UK 2020-10-02 /pmc/articles/PMC7532536/ /pubmed/33009486 http://dx.doi.org/10.1038/s41598-020-73414-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Paun, I. A.
Mustaciosu, C. C.
Mihailescu, M.
Calin, B. S.
Sandu, A. M.
Magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing
title Magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing
title_full Magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing
title_fullStr Magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing
title_full_unstemmed Magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing
title_short Magnetically-driven 2D cells organization on superparamagnetic micromagnets fabricated by laser direct writing
title_sort magnetically-driven 2d cells organization on superparamagnetic micromagnets fabricated by laser direct writing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532536/
https://www.ncbi.nlm.nih.gov/pubmed/33009486
http://dx.doi.org/10.1038/s41598-020-73414-4
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