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CRISPR-Sunspot: Imaging of endogenous low-abundance RNA at the single-molecule level in live cells
CRISPR/Cas-based mRNA imaging has been developed to labeling of high-abundance mRNAs. A lack of non-genetically encoded mRNA-tagged imaging tools has limited our ability to explore the functional distributions of endogenous low-abundance mRNAs in cells. Here, we developed a CRISPR-Sunspot method bas...
Autores principales: | Sun, Ning-He, Chen, Dan-Yang, Ye, Lu-Peng, Sheng, Gang, Gong, Jun-Jie, Chen, Bao-Hui, Lu, Ying-Mei, Han, Feng |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532675/ https://www.ncbi.nlm.nih.gov/pubmed/33042266 http://dx.doi.org/10.7150/thno.43094 |
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