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The nine ADAMs family members serve as potential biomarkers for immune infiltration in pancreatic adenocarcinoma
BACKGROUND: The functional significance of ADAMs family members in the immune infiltration of pancreatic adenocarcinoma (PAAD) awaits elucidation. METHODS: ADAMs family members with significant expression were identified among differentially expressed genes of PAAD based on The Cancer Genome Atlas (...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532768/ https://www.ncbi.nlm.nih.gov/pubmed/33062410 http://dx.doi.org/10.7717/peerj.9736 |
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author | Qi, Bing Liu, Han Dong, Ying Shi, Xueying Zhou, Qi Zeng, Fen Bao, Nabuqi Li, Qian Yuan, Yuan Yao, Lei Xia, Shilin |
author_facet | Qi, Bing Liu, Han Dong, Ying Shi, Xueying Zhou, Qi Zeng, Fen Bao, Nabuqi Li, Qian Yuan, Yuan Yao, Lei Xia, Shilin |
author_sort | Qi, Bing |
collection | PubMed |
description | BACKGROUND: The functional significance of ADAMs family members in the immune infiltration of pancreatic adenocarcinoma (PAAD) awaits elucidation. METHODS: ADAMs family members with significant expression were identified among differentially expressed genes of PAAD based on The Cancer Genome Atlas (TCGA) database followed by a verification based on the Oncomine database. The correlation of ADAMs in PAAD was estimated with the Spearman’s rho value. The pathway enrichment of ADAMs was performed by STRING and GSEALite, respectively. The protein–protein interaction and Gene Ontology analyses of ADAMs and their similar genes were exanimated in STRING and visualized by Cytoscape. Subsequently, the Box-Whisker plot was used to show a correlation between ADAMs and different tumor grade 1/2/3/4 with Student’s t-test. TIMER was applied to estimate a correlation of ADAMs expressions with immune infiltrates and immune checkpoint blockade (ICB) immunotherapy-related molecules. Furthermore, the effect of copy number variation (CNV) of ADAMs genes was assessed on the immune infiltration levels. RESULT: ADAM8/9/10/12/15/19/28/TS2/TS12 were over-expressed in PAAD. Most of the nine ADAMs had a significant correlation. ADAM8/12/15/19 expression was remarkably increased in the comparison between grade 1 and grade 2/3 of PAAD. ADAM8/9/10/12/19/28/TS2/TS12 had a positive correlation with almost five immune infiltrates. ADAM12/19/TS2/TS12 dramatically related with ICB immunotherapy-related molecules. CNV of ADAMs genes potentially influenced the immune infiltration levels. CONCLUSION: Knowledge of the expression level of the ADAMs family could provide a reasonable strategy for improved immunotherapies to PAAD. |
format | Online Article Text |
id | pubmed-7532768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75327682020-10-13 The nine ADAMs family members serve as potential biomarkers for immune infiltration in pancreatic adenocarcinoma Qi, Bing Liu, Han Dong, Ying Shi, Xueying Zhou, Qi Zeng, Fen Bao, Nabuqi Li, Qian Yuan, Yuan Yao, Lei Xia, Shilin PeerJ Bioinformatics BACKGROUND: The functional significance of ADAMs family members in the immune infiltration of pancreatic adenocarcinoma (PAAD) awaits elucidation. METHODS: ADAMs family members with significant expression were identified among differentially expressed genes of PAAD based on The Cancer Genome Atlas (TCGA) database followed by a verification based on the Oncomine database. The correlation of ADAMs in PAAD was estimated with the Spearman’s rho value. The pathway enrichment of ADAMs was performed by STRING and GSEALite, respectively. The protein–protein interaction and Gene Ontology analyses of ADAMs and their similar genes were exanimated in STRING and visualized by Cytoscape. Subsequently, the Box-Whisker plot was used to show a correlation between ADAMs and different tumor grade 1/2/3/4 with Student’s t-test. TIMER was applied to estimate a correlation of ADAMs expressions with immune infiltrates and immune checkpoint blockade (ICB) immunotherapy-related molecules. Furthermore, the effect of copy number variation (CNV) of ADAMs genes was assessed on the immune infiltration levels. RESULT: ADAM8/9/10/12/15/19/28/TS2/TS12 were over-expressed in PAAD. Most of the nine ADAMs had a significant correlation. ADAM8/12/15/19 expression was remarkably increased in the comparison between grade 1 and grade 2/3 of PAAD. ADAM8/9/10/12/19/28/TS2/TS12 had a positive correlation with almost five immune infiltrates. ADAM12/19/TS2/TS12 dramatically related with ICB immunotherapy-related molecules. CNV of ADAMs genes potentially influenced the immune infiltration levels. CONCLUSION: Knowledge of the expression level of the ADAMs family could provide a reasonable strategy for improved immunotherapies to PAAD. PeerJ Inc. 2020-09-30 /pmc/articles/PMC7532768/ /pubmed/33062410 http://dx.doi.org/10.7717/peerj.9736 Text en ©2020 Qi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Bioinformatics Qi, Bing Liu, Han Dong, Ying Shi, Xueying Zhou, Qi Zeng, Fen Bao, Nabuqi Li, Qian Yuan, Yuan Yao, Lei Xia, Shilin The nine ADAMs family members serve as potential biomarkers for immune infiltration in pancreatic adenocarcinoma |
title | The nine ADAMs family members serve as potential biomarkers for immune infiltration in pancreatic adenocarcinoma |
title_full | The nine ADAMs family members serve as potential biomarkers for immune infiltration in pancreatic adenocarcinoma |
title_fullStr | The nine ADAMs family members serve as potential biomarkers for immune infiltration in pancreatic adenocarcinoma |
title_full_unstemmed | The nine ADAMs family members serve as potential biomarkers for immune infiltration in pancreatic adenocarcinoma |
title_short | The nine ADAMs family members serve as potential biomarkers for immune infiltration in pancreatic adenocarcinoma |
title_sort | nine adams family members serve as potential biomarkers for immune infiltration in pancreatic adenocarcinoma |
topic | Bioinformatics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532768/ https://www.ncbi.nlm.nih.gov/pubmed/33062410 http://dx.doi.org/10.7717/peerj.9736 |
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