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Molecular Epidemiology and Risk Factors of Blastocystis sp. Infections Among General Populations in Yunnan Province, Southwestern China

BACKGROUND: Blastocystis is a common enteric parasite of controversial pathogenic roles in human diseases. Although the prevalence of Blastocystis infections has been investigated in a diverse range of populations, there is little knowledge on the molecular epidemiology and risk factors of Blastocys...

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Autores principales: Deng, Yao, Zhang, Shunxian, Ning, Chaoqun, Zhou, Yongkang, Teng, Xuejiao, Wu, Xiuping, Chu, Yanhong, Yu, Yingfang, Chen, Jiaxu, Tian, Liguang, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532910/
https://www.ncbi.nlm.nih.gov/pubmed/33061712
http://dx.doi.org/10.2147/RMHP.S269664
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author Deng, Yao
Zhang, Shunxian
Ning, Chaoqun
Zhou, Yongkang
Teng, Xuejiao
Wu, Xiuping
Chu, Yanhong
Yu, Yingfang
Chen, Jiaxu
Tian, Liguang
Wang, Wei
author_facet Deng, Yao
Zhang, Shunxian
Ning, Chaoqun
Zhou, Yongkang
Teng, Xuejiao
Wu, Xiuping
Chu, Yanhong
Yu, Yingfang
Chen, Jiaxu
Tian, Liguang
Wang, Wei
author_sort Deng, Yao
collection PubMed
description BACKGROUND: Blastocystis is a common enteric parasite of controversial pathogenic roles in human diseases. Although the prevalence of Blastocystis infections has been investigated in a diverse range of populations, there is little knowledge on the molecular epidemiology and risk factors of Blastocystis infections among general populations in southeastern China. MATERIALS AND METHODS: A total of 507 individuals were randomly selected in Yunnan province, China from July 2016 to March 2017. Stool specimens were sampled for detection of Blastocystis sp. using PCR assay, and the risk factors of Blastocystis infections were identified. Blastocystis isolates were subtyped, and the associations of Blastocystis infections and subtypes with clinical manifestations were examined. RESULTS: The overall detection rate of Blastocystis sp. was 9.47% (95% CI: 7.13–12.44%). Toilet type (OR = 3.248, 95% CI: 1.245–8.473), anemia (OR = 2.601, 95% CI: 1.245–8.473) and type of daily drinking water (OR = 3.11, 95% CI: 1.557–6.213) were identified as risk factors of Blastocystis infections; however, Blastocystis infections showed no associations with clinical symptoms. Four subtypes (ST1 to ST4) were characterized in Blastocystis isolates, in which ST3 was predominant (4.73%, 95% CI: 3.2–6.94%), followed by ST1 (3.16%, 95% CI: 1.95–5.07%), ST4 (1.38%, 95% CI: 0.07–2.82%) and ST2 (0.2%, 95% CI: 0–1.11%). In addition, ST1 subtype infection was found to correlate with anemia (OR = 4.66, 95% CI: 1.631–14.314). CONCLUSIONS: There is a high prevalence of Blastocystis infections among general populations in Yunnan province, southwestern China, and toilet type, anemia and type of daily drinking water are risk factors of Blastocystis infections. ST3 is the dominant subtype of Blastocystis sp. characterized, and ST1 correlates with anemia. Improving hygiene conditions, developing healthy lifestyles and intensifying health education programs are strongly recommended to reduce the prevalence and transmission potential of Blastocystis infections.
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spelling pubmed-75329102020-10-14 Molecular Epidemiology and Risk Factors of Blastocystis sp. Infections Among General Populations in Yunnan Province, Southwestern China Deng, Yao Zhang, Shunxian Ning, Chaoqun Zhou, Yongkang Teng, Xuejiao Wu, Xiuping Chu, Yanhong Yu, Yingfang Chen, Jiaxu Tian, Liguang Wang, Wei Risk Manag Healthc Policy Original Research BACKGROUND: Blastocystis is a common enteric parasite of controversial pathogenic roles in human diseases. Although the prevalence of Blastocystis infections has been investigated in a diverse range of populations, there is little knowledge on the molecular epidemiology and risk factors of Blastocystis infections among general populations in southeastern China. MATERIALS AND METHODS: A total of 507 individuals were randomly selected in Yunnan province, China from July 2016 to March 2017. Stool specimens were sampled for detection of Blastocystis sp. using PCR assay, and the risk factors of Blastocystis infections were identified. Blastocystis isolates were subtyped, and the associations of Blastocystis infections and subtypes with clinical manifestations were examined. RESULTS: The overall detection rate of Blastocystis sp. was 9.47% (95% CI: 7.13–12.44%). Toilet type (OR = 3.248, 95% CI: 1.245–8.473), anemia (OR = 2.601, 95% CI: 1.245–8.473) and type of daily drinking water (OR = 3.11, 95% CI: 1.557–6.213) were identified as risk factors of Blastocystis infections; however, Blastocystis infections showed no associations with clinical symptoms. Four subtypes (ST1 to ST4) were characterized in Blastocystis isolates, in which ST3 was predominant (4.73%, 95% CI: 3.2–6.94%), followed by ST1 (3.16%, 95% CI: 1.95–5.07%), ST4 (1.38%, 95% CI: 0.07–2.82%) and ST2 (0.2%, 95% CI: 0–1.11%). In addition, ST1 subtype infection was found to correlate with anemia (OR = 4.66, 95% CI: 1.631–14.314). CONCLUSIONS: There is a high prevalence of Blastocystis infections among general populations in Yunnan province, southwestern China, and toilet type, anemia and type of daily drinking water are risk factors of Blastocystis infections. ST3 is the dominant subtype of Blastocystis sp. characterized, and ST1 correlates with anemia. Improving hygiene conditions, developing healthy lifestyles and intensifying health education programs are strongly recommended to reduce the prevalence and transmission potential of Blastocystis infections. Dove 2020-09-29 /pmc/articles/PMC7532910/ /pubmed/33061712 http://dx.doi.org/10.2147/RMHP.S269664 Text en © 2020 Deng et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Deng, Yao
Zhang, Shunxian
Ning, Chaoqun
Zhou, Yongkang
Teng, Xuejiao
Wu, Xiuping
Chu, Yanhong
Yu, Yingfang
Chen, Jiaxu
Tian, Liguang
Wang, Wei
Molecular Epidemiology and Risk Factors of Blastocystis sp. Infections Among General Populations in Yunnan Province, Southwestern China
title Molecular Epidemiology and Risk Factors of Blastocystis sp. Infections Among General Populations in Yunnan Province, Southwestern China
title_full Molecular Epidemiology and Risk Factors of Blastocystis sp. Infections Among General Populations in Yunnan Province, Southwestern China
title_fullStr Molecular Epidemiology and Risk Factors of Blastocystis sp. Infections Among General Populations in Yunnan Province, Southwestern China
title_full_unstemmed Molecular Epidemiology and Risk Factors of Blastocystis sp. Infections Among General Populations in Yunnan Province, Southwestern China
title_short Molecular Epidemiology and Risk Factors of Blastocystis sp. Infections Among General Populations in Yunnan Province, Southwestern China
title_sort molecular epidemiology and risk factors of blastocystis sp. infections among general populations in yunnan province, southwestern china
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532910/
https://www.ncbi.nlm.nih.gov/pubmed/33061712
http://dx.doi.org/10.2147/RMHP.S269664
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