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Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics

The cyanobacterium Synechococcus elongatus (SE) has been shown to rescue ischaemic heart muscle after myocardial infarction by photosynthetic oxygen production. Here, we investigated SE toxicity and hypothesized that systemic SE exposure does not elicit a significant immune response in rats. Wistar...

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Autores principales: Williams, Kiah M., Wang, Hanjay, Paulsen, Michael J., Thakore, Akshara D., Rieck, Mary, Lucian, Haley J., Grady, Frederick, Hironaka, Camille E., Chien, Athena J., Farry, Justin M., Shin, Hye Sook, Jaatinen, Kevin J., Eskandari, Anahita, Stapleton, Lyndsay M., Steele, Amanda N., Cohen, Jeffrey E., Woo, Y. Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533327/
https://www.ncbi.nlm.nih.gov/pubmed/32476224
http://dx.doi.org/10.1111/1751-7915.13596
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author Williams, Kiah M.
Wang, Hanjay
Paulsen, Michael J.
Thakore, Akshara D.
Rieck, Mary
Lucian, Haley J.
Grady, Frederick
Hironaka, Camille E.
Chien, Athena J.
Farry, Justin M.
Shin, Hye Sook
Jaatinen, Kevin J.
Eskandari, Anahita
Stapleton, Lyndsay M.
Steele, Amanda N.
Cohen, Jeffrey E.
Woo, Y. Joseph
author_facet Williams, Kiah M.
Wang, Hanjay
Paulsen, Michael J.
Thakore, Akshara D.
Rieck, Mary
Lucian, Haley J.
Grady, Frederick
Hironaka, Camille E.
Chien, Athena J.
Farry, Justin M.
Shin, Hye Sook
Jaatinen, Kevin J.
Eskandari, Anahita
Stapleton, Lyndsay M.
Steele, Amanda N.
Cohen, Jeffrey E.
Woo, Y. Joseph
author_sort Williams, Kiah M.
collection PubMed
description The cyanobacterium Synechococcus elongatus (SE) has been shown to rescue ischaemic heart muscle after myocardial infarction by photosynthetic oxygen production. Here, we investigated SE toxicity and hypothesized that systemic SE exposure does not elicit a significant immune response in rats. Wistar rats intravenously received SE (n = 12), sterile saline (n = 12) or E. coli lipopolysaccharide (LPS, n = 4), and a subset (8 SE, 8 saline) received a repeat injection 4 weeks later. At baseline, 4 h, 24 h, 48 h, 8 days and 4 weeks after injection, clinical assessments, blood cultures, blood counts, lymphocyte phenotypes, liver function tests, proinflammatory cytokines and immunoglobulins were assessed. Across all metrics, SE rats responded comparably to saline controls, displaying no clinically significant immune response. As expected, LPS rats exhibited severe immunological responses. Systemic SE administration does not induce sepsis or toxicity in rats, thereby supporting the safety of cyanobacteria–mammalian symbiotic therapeutics using this organism.
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spelling pubmed-75333272020-10-07 Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics Williams, Kiah M. Wang, Hanjay Paulsen, Michael J. Thakore, Akshara D. Rieck, Mary Lucian, Haley J. Grady, Frederick Hironaka, Camille E. Chien, Athena J. Farry, Justin M. Shin, Hye Sook Jaatinen, Kevin J. Eskandari, Anahita Stapleton, Lyndsay M. Steele, Amanda N. Cohen, Jeffrey E. Woo, Y. Joseph Microb Biotechnol Research Articles The cyanobacterium Synechococcus elongatus (SE) has been shown to rescue ischaemic heart muscle after myocardial infarction by photosynthetic oxygen production. Here, we investigated SE toxicity and hypothesized that systemic SE exposure does not elicit a significant immune response in rats. Wistar rats intravenously received SE (n = 12), sterile saline (n = 12) or E. coli lipopolysaccharide (LPS, n = 4), and a subset (8 SE, 8 saline) received a repeat injection 4 weeks later. At baseline, 4 h, 24 h, 48 h, 8 days and 4 weeks after injection, clinical assessments, blood cultures, blood counts, lymphocyte phenotypes, liver function tests, proinflammatory cytokines and immunoglobulins were assessed. Across all metrics, SE rats responded comparably to saline controls, displaying no clinically significant immune response. As expected, LPS rats exhibited severe immunological responses. Systemic SE administration does not induce sepsis or toxicity in rats, thereby supporting the safety of cyanobacteria–mammalian symbiotic therapeutics using this organism. John Wiley and Sons Inc. 2020-05-31 /pmc/articles/PMC7533327/ /pubmed/32476224 http://dx.doi.org/10.1111/1751-7915.13596 Text en © 2020 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Williams, Kiah M.
Wang, Hanjay
Paulsen, Michael J.
Thakore, Akshara D.
Rieck, Mary
Lucian, Haley J.
Grady, Frederick
Hironaka, Camille E.
Chien, Athena J.
Farry, Justin M.
Shin, Hye Sook
Jaatinen, Kevin J.
Eskandari, Anahita
Stapleton, Lyndsay M.
Steele, Amanda N.
Cohen, Jeffrey E.
Woo, Y. Joseph
Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics
title Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics
title_full Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics
title_fullStr Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics
title_full_unstemmed Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics
title_short Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics
title_sort safety of photosynthetic synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533327/
https://www.ncbi.nlm.nih.gov/pubmed/32476224
http://dx.doi.org/10.1111/1751-7915.13596
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