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Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry

Unstable angina (UA) is a coronary disease with a high mortality and morbidity worldwide. The present study aimed to use non-invasive techniques to identify urine biomarkers in patients with UA, so as to provide more information for the early diagnosis and treatment of the disease. Based on metabolo...

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Autores principales: Liu, Yuechen, Li, Yue, Zhang, Tianpu, Zhao, Huan, Fan, Simiao, Cai, Xuemeng, Liu, Yijia, Li, Zhu, Gao, Shan, Li, Yubo, Yu, Chunquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533448/
https://www.ncbi.nlm.nih.gov/pubmed/32901869
http://dx.doi.org/10.3892/mmr.2020.11476
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author Liu, Yuechen
Li, Yue
Zhang, Tianpu
Zhao, Huan
Fan, Simiao
Cai, Xuemeng
Liu, Yijia
Li, Zhu
Gao, Shan
Li, Yubo
Yu, Chunquan
author_facet Liu, Yuechen
Li, Yue
Zhang, Tianpu
Zhao, Huan
Fan, Simiao
Cai, Xuemeng
Liu, Yijia
Li, Zhu
Gao, Shan
Li, Yubo
Yu, Chunquan
author_sort Liu, Yuechen
collection PubMed
description Unstable angina (UA) is a coronary disease with a high mortality and morbidity worldwide. The present study aimed to use non-invasive techniques to identify urine biomarkers in patients with UA, so as to provide more information for the early diagnosis and treatment of the disease. Based on metabolomics, urine samples from 28 patients with UA and 28 healthy controls (HCs) were analyzed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). A total of 16 significant biomarkers that could distinguish between patients with UA and HCs, including D-glucuronic acid, creatinine, succinic acid and N-acetylneuraminic acid, were identified. The major metabolic pathways associated with UA were subsequently analyzed by non-targeted metabolomics. The results demonstrated that amino acid and energy metabolism, fatty acid metabolism, purine metabolism and steroid hormone biosynthetic metabolism may serve important roles in UA. The results of the current study may provide a theoretical basis for the early diagnosis of UA and novel treatment strategies for clinicians. The trial was registered with the Chinese Clinical Trial Registration Center (registration no. ChiCTR-ROC-17013957) at Tianjin University of Traditional Chinese Medicine.
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spelling pubmed-75334482020-10-07 Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry Liu, Yuechen Li, Yue Zhang, Tianpu Zhao, Huan Fan, Simiao Cai, Xuemeng Liu, Yijia Li, Zhu Gao, Shan Li, Yubo Yu, Chunquan Mol Med Rep Articles Unstable angina (UA) is a coronary disease with a high mortality and morbidity worldwide. The present study aimed to use non-invasive techniques to identify urine biomarkers in patients with UA, so as to provide more information for the early diagnosis and treatment of the disease. Based on metabolomics, urine samples from 28 patients with UA and 28 healthy controls (HCs) were analyzed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). A total of 16 significant biomarkers that could distinguish between patients with UA and HCs, including D-glucuronic acid, creatinine, succinic acid and N-acetylneuraminic acid, were identified. The major metabolic pathways associated with UA were subsequently analyzed by non-targeted metabolomics. The results demonstrated that amino acid and energy metabolism, fatty acid metabolism, purine metabolism and steroid hormone biosynthetic metabolism may serve important roles in UA. The results of the current study may provide a theoretical basis for the early diagnosis of UA and novel treatment strategies for clinicians. The trial was registered with the Chinese Clinical Trial Registration Center (registration no. ChiCTR-ROC-17013957) at Tianjin University of Traditional Chinese Medicine. D.A. Spandidos 2020-11 2020-09-02 /pmc/articles/PMC7533448/ /pubmed/32901869 http://dx.doi.org/10.3892/mmr.2020.11476 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Yuechen
Li, Yue
Zhang, Tianpu
Zhao, Huan
Fan, Simiao
Cai, Xuemeng
Liu, Yijia
Li, Zhu
Gao, Shan
Li, Yubo
Yu, Chunquan
Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
title Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
title_full Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
title_fullStr Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
title_full_unstemmed Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
title_short Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
title_sort analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533448/
https://www.ncbi.nlm.nih.gov/pubmed/32901869
http://dx.doi.org/10.3892/mmr.2020.11476
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