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MicroRNA-214 promotes the EMT process in melanoma by downregulating CADM1 expression
Melanoma is a malignant skin cancer type associated with a high mortality rate, but its treatment is currently not ideal. Both microRNA (miR)-214 and cell adhesion molecule 1 (CADM1) are differentially expressed in melanoma, but their role in this cancer type remains unknown. Therefore, the aim of t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533494/ https://www.ncbi.nlm.nih.gov/pubmed/33000202 http://dx.doi.org/10.3892/mmr.2020.11446 |
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author | Wang, Shu-Jun Li, Wei-Wei Wen, Cong-Ji Diao, Yong-Li Zhao, Tian-Lan |
author_facet | Wang, Shu-Jun Li, Wei-Wei Wen, Cong-Ji Diao, Yong-Li Zhao, Tian-Lan |
author_sort | Wang, Shu-Jun |
collection | PubMed |
description | Melanoma is a malignant skin cancer type associated with a high mortality rate, but its treatment is currently not ideal. Both microRNA (miR)-214 and cell adhesion molecule 1 (CADM1) are differentially expressed in melanoma, but their role in this cancer type remains unknown. Therefore, the aim of the present study was to investigate the role of CADM1 and miR-214 in melanoma to identify novel targets for its treatment. The expression levels of CADM1 and miR-214 in cells were detected by reverse transcription-quantitative PCR (RT-qPCR). Moreover, cell viability, migration and invasion were measured by MTT, wound healing and Transwell assays, respectively. In addition, the relative expression levels of epithelial-mesenchymal transition (EMT)-related proteins in cells were detected by RT-qPCR and western blotting. It was found that the expression of CADM1 was inhibited in melanoma cells, while miR-214 expression was increased during melanoma tumorigenesis. Furthermore, miR-214 mimics promoted the viability, migration and invasion of melanoma cells. It was also demonstrated that the downregulation of CADM1 reversed the inhibitory effect of the miR-214 inhibitor in melanoma. Moreover, overexpression of CADM1 inhibited the EMT process in melanoma, while the miR-214 inhibitor suppressed the EMT process. The results also indicated that miR-214 promoted the EMT process by downregulating CADM1, which may represent a novel mechanism for the progression of melanoma. |
format | Online Article Text |
id | pubmed-7533494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-75334942020-10-07 MicroRNA-214 promotes the EMT process in melanoma by downregulating CADM1 expression Wang, Shu-Jun Li, Wei-Wei Wen, Cong-Ji Diao, Yong-Li Zhao, Tian-Lan Mol Med Rep Articles Melanoma is a malignant skin cancer type associated with a high mortality rate, but its treatment is currently not ideal. Both microRNA (miR)-214 and cell adhesion molecule 1 (CADM1) are differentially expressed in melanoma, but their role in this cancer type remains unknown. Therefore, the aim of the present study was to investigate the role of CADM1 and miR-214 in melanoma to identify novel targets for its treatment. The expression levels of CADM1 and miR-214 in cells were detected by reverse transcription-quantitative PCR (RT-qPCR). Moreover, cell viability, migration and invasion were measured by MTT, wound healing and Transwell assays, respectively. In addition, the relative expression levels of epithelial-mesenchymal transition (EMT)-related proteins in cells were detected by RT-qPCR and western blotting. It was found that the expression of CADM1 was inhibited in melanoma cells, while miR-214 expression was increased during melanoma tumorigenesis. Furthermore, miR-214 mimics promoted the viability, migration and invasion of melanoma cells. It was also demonstrated that the downregulation of CADM1 reversed the inhibitory effect of the miR-214 inhibitor in melanoma. Moreover, overexpression of CADM1 inhibited the EMT process in melanoma, while the miR-214 inhibitor suppressed the EMT process. The results also indicated that miR-214 promoted the EMT process by downregulating CADM1, which may represent a novel mechanism for the progression of melanoma. D.A. Spandidos 2020-11 2020-08-20 /pmc/articles/PMC7533494/ /pubmed/33000202 http://dx.doi.org/10.3892/mmr.2020.11446 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wang, Shu-Jun Li, Wei-Wei Wen, Cong-Ji Diao, Yong-Li Zhao, Tian-Lan MicroRNA-214 promotes the EMT process in melanoma by downregulating CADM1 expression |
title | MicroRNA-214 promotes the EMT process in melanoma by downregulating CADM1 expression |
title_full | MicroRNA-214 promotes the EMT process in melanoma by downregulating CADM1 expression |
title_fullStr | MicroRNA-214 promotes the EMT process in melanoma by downregulating CADM1 expression |
title_full_unstemmed | MicroRNA-214 promotes the EMT process in melanoma by downregulating CADM1 expression |
title_short | MicroRNA-214 promotes the EMT process in melanoma by downregulating CADM1 expression |
title_sort | microrna-214 promotes the emt process in melanoma by downregulating cadm1 expression |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533494/ https://www.ncbi.nlm.nih.gov/pubmed/33000202 http://dx.doi.org/10.3892/mmr.2020.11446 |
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