Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization

Biofilm formation is important for establishing plants-microbe associations. The role of calcium on biofilm formation has been studied in many bacteria except rhizobia. In this study, we investigated the role of calcium for biofilm formation in Azorhizobium caulindans, which forms nodules in the ste...

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Autores principales: Liu, Xiaolin, Zhang, Kaiye, Liu, Yanan, Zou, Desheng, Wang, Dandan, Xie, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533552/
https://www.ncbi.nlm.nih.gov/pubmed/33072026
http://dx.doi.org/10.3389/fmicb.2020.563367
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author Liu, Xiaolin
Zhang, Kaiye
Liu, Yanan
Zou, Desheng
Wang, Dandan
Xie, Zhihong
author_facet Liu, Xiaolin
Zhang, Kaiye
Liu, Yanan
Zou, Desheng
Wang, Dandan
Xie, Zhihong
author_sort Liu, Xiaolin
collection PubMed
description Biofilm formation is important for establishing plants-microbe associations. The role of calcium on biofilm formation has been studied in many bacteria except rhizobia. In this study, we investigated the role of calcium for biofilm formation in Azorhizobium caulindans, which forms nodules in the stem and root of its host plant Sesbania rostrata. We found that calcium is essential for A. caulindans biofilm formation, in addition to the presence of extracellular matrix components, eDNA and proteins. Also, calcium-mediated biofilm formation was tested with chemotaxis, motility, cyclic di-GMP synthesis, and quorum sensing mutants. Finally, calcium was found to promote S. rostrata root colonization of A. caulinodans. In total, these results show that calcium is essential for A. caulindans biofilm formation, and it affects the interaction between A. caulinodans and host plant.
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spelling pubmed-75335522020-10-16 Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization Liu, Xiaolin Zhang, Kaiye Liu, Yanan Zou, Desheng Wang, Dandan Xie, Zhihong Front Microbiol Microbiology Biofilm formation is important for establishing plants-microbe associations. The role of calcium on biofilm formation has been studied in many bacteria except rhizobia. In this study, we investigated the role of calcium for biofilm formation in Azorhizobium caulindans, which forms nodules in the stem and root of its host plant Sesbania rostrata. We found that calcium is essential for A. caulindans biofilm formation, in addition to the presence of extracellular matrix components, eDNA and proteins. Also, calcium-mediated biofilm formation was tested with chemotaxis, motility, cyclic di-GMP synthesis, and quorum sensing mutants. Finally, calcium was found to promote S. rostrata root colonization of A. caulinodans. In total, these results show that calcium is essential for A. caulindans biofilm formation, and it affects the interaction between A. caulinodans and host plant. Frontiers Media S.A. 2020-09-16 /pmc/articles/PMC7533552/ /pubmed/33072026 http://dx.doi.org/10.3389/fmicb.2020.563367 Text en Copyright © 2020 Liu, Zhang, Liu, Zou, Wang and Xie. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Xiaolin
Zhang, Kaiye
Liu, Yanan
Zou, Desheng
Wang, Dandan
Xie, Zhihong
Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization
title Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization
title_full Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization
title_fullStr Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization
title_full_unstemmed Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization
title_short Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization
title_sort effects of calcium and signal sensing systems on azorhizobium caulinodans biofilm formation and host colonization
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533552/
https://www.ncbi.nlm.nih.gov/pubmed/33072026
http://dx.doi.org/10.3389/fmicb.2020.563367
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