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Differential Transcription and Alternative Splicing in Cotton Underly Specialized Defense Responses Against Pests
The green mirid bug (Apolygus lucorum) and the cotton bollworm (Helicoverpa armigera) are both preferred to live on cotton but cause different symptoms, suggesting specialized responses of cotton to the two insects. In this study, we investigated differential molecular mechanisms underlying cotton p...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533563/ https://www.ncbi.nlm.nih.gov/pubmed/33072149 http://dx.doi.org/10.3389/fpls.2020.573131 |
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author | Chen, Dian-Yang Chen, Qiu-Yi Wang, Dan-Dan Mu, Yu-Pei Wang, Mu-Yang Huang, Ji-Rong Mao, Ying-Bo |
author_facet | Chen, Dian-Yang Chen, Qiu-Yi Wang, Dan-Dan Mu, Yu-Pei Wang, Mu-Yang Huang, Ji-Rong Mao, Ying-Bo |
author_sort | Chen, Dian-Yang |
collection | PubMed |
description | The green mirid bug (Apolygus lucorum) and the cotton bollworm (Helicoverpa armigera) are both preferred to live on cotton but cause different symptoms, suggesting specialized responses of cotton to the two insects. In this study, we investigated differential molecular mechanisms underlying cotton plant defenses against A. lucorum and H. armigera via transcriptomic analyses. At the transcription level, jasmonate (JA) signaling was dominated in defense against H. armigera whereas salicylic acid (SA) signaling was more significant in defense against A. lucorum. A set of pathogenesis-related (PR) genes and protease inhibitor genes were differentially induced by the two insects. Insect infestations also had an impact on alternative splicing (AS), which was altered more significantly by the H. armigera than A. lucorum. Interestingly, most differential AS (DAS) genes had no obvious change at the transcription level. GO analysis revealed that biological process termed “RNA splicing” and “cellular response to abiotic stimulus” were enriched only in DAS genes from the H. armigera infested samples. Furthermore, insect infestations induced the retained intron of GhJAZs transcripts, which produced a truncated protein lacking the intact Jas motif. Taken together, our data demonstrate that the specialized cotton response to different insects is regulated by gene transcription and AS as well. |
format | Online Article Text |
id | pubmed-7533563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75335632020-10-15 Differential Transcription and Alternative Splicing in Cotton Underly Specialized Defense Responses Against Pests Chen, Dian-Yang Chen, Qiu-Yi Wang, Dan-Dan Mu, Yu-Pei Wang, Mu-Yang Huang, Ji-Rong Mao, Ying-Bo Front Plant Sci Plant Science The green mirid bug (Apolygus lucorum) and the cotton bollworm (Helicoverpa armigera) are both preferred to live on cotton but cause different symptoms, suggesting specialized responses of cotton to the two insects. In this study, we investigated differential molecular mechanisms underlying cotton plant defenses against A. lucorum and H. armigera via transcriptomic analyses. At the transcription level, jasmonate (JA) signaling was dominated in defense against H. armigera whereas salicylic acid (SA) signaling was more significant in defense against A. lucorum. A set of pathogenesis-related (PR) genes and protease inhibitor genes were differentially induced by the two insects. Insect infestations also had an impact on alternative splicing (AS), which was altered more significantly by the H. armigera than A. lucorum. Interestingly, most differential AS (DAS) genes had no obvious change at the transcription level. GO analysis revealed that biological process termed “RNA splicing” and “cellular response to abiotic stimulus” were enriched only in DAS genes from the H. armigera infested samples. Furthermore, insect infestations induced the retained intron of GhJAZs transcripts, which produced a truncated protein lacking the intact Jas motif. Taken together, our data demonstrate that the specialized cotton response to different insects is regulated by gene transcription and AS as well. Frontiers Media S.A. 2020-09-15 /pmc/articles/PMC7533563/ /pubmed/33072149 http://dx.doi.org/10.3389/fpls.2020.573131 Text en Copyright © 2020 Chen, Chen, Wang, Mu, Wang, Huang and Mao http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Chen, Dian-Yang Chen, Qiu-Yi Wang, Dan-Dan Mu, Yu-Pei Wang, Mu-Yang Huang, Ji-Rong Mao, Ying-Bo Differential Transcription and Alternative Splicing in Cotton Underly Specialized Defense Responses Against Pests |
title | Differential Transcription and Alternative Splicing in Cotton Underly Specialized Defense Responses Against Pests |
title_full | Differential Transcription and Alternative Splicing in Cotton Underly Specialized Defense Responses Against Pests |
title_fullStr | Differential Transcription and Alternative Splicing in Cotton Underly Specialized Defense Responses Against Pests |
title_full_unstemmed | Differential Transcription and Alternative Splicing in Cotton Underly Specialized Defense Responses Against Pests |
title_short | Differential Transcription and Alternative Splicing in Cotton Underly Specialized Defense Responses Against Pests |
title_sort | differential transcription and alternative splicing in cotton underly specialized defense responses against pests |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533563/ https://www.ncbi.nlm.nih.gov/pubmed/33072149 http://dx.doi.org/10.3389/fpls.2020.573131 |
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