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MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3

microRNA-663a (miR-663a) was reported to be highly expressed in cancers. However, its roles in melanoma progression remain unclear. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted to measure miR-663a expression level in melanoma cell lines and normal cells. Cell...

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Detalles Bibliográficos
Autores principales: Liu, Saijun, Hu, Yunfeng, Wu, Shi, He, Yong, Deng, Liehua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533922/
https://www.ncbi.nlm.nih.gov/pubmed/33000682
http://dx.doi.org/10.1177/1533033820957000
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author Liu, Saijun
Hu, Yunfeng
Wu, Shi
He, Yong
Deng, Liehua
author_facet Liu, Saijun
Hu, Yunfeng
Wu, Shi
He, Yong
Deng, Liehua
author_sort Liu, Saijun
collection PubMed
description microRNA-663a (miR-663a) was reported to be highly expressed in cancers. However, its roles in melanoma progression remain unclear. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted to measure miR-663a expression level in melanoma cell lines and normal cells. Cell counting kit-8 assay, wound-healing assay, and transwell invasion assay were conducted to analyze biological roles of miR-663a in melanoma. Luciferase activity reporter assay was conducted to validate the connection of miR-663a and Four and a half LIM domain (FHL) protein 3 (FHL3) in melanoma. Our results showed miR-663a expression level was significantly increased in melanoma cells compared with normal cells. Silencing miR-663a expression suppresses melanoma cell proliferation, migration, and invasion in vitro. Moreover, FHL3 was validated as a functional target of miR-663a. Knockdown of FHL3 partially rescued the inhibitory effects of miR-663a inhibitor on melanoma cell behaviors. Together, our work provided evidence that miR-663a functions as an oncogenic miRNA in melanoma.
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spelling pubmed-75339222020-10-14 MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3 Liu, Saijun Hu, Yunfeng Wu, Shi He, Yong Deng, Liehua Technol Cancer Res Treat Original Article microRNA-663a (miR-663a) was reported to be highly expressed in cancers. However, its roles in melanoma progression remain unclear. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted to measure miR-663a expression level in melanoma cell lines and normal cells. Cell counting kit-8 assay, wound-healing assay, and transwell invasion assay were conducted to analyze biological roles of miR-663a in melanoma. Luciferase activity reporter assay was conducted to validate the connection of miR-663a and Four and a half LIM domain (FHL) protein 3 (FHL3) in melanoma. Our results showed miR-663a expression level was significantly increased in melanoma cells compared with normal cells. Silencing miR-663a expression suppresses melanoma cell proliferation, migration, and invasion in vitro. Moreover, FHL3 was validated as a functional target of miR-663a. Knockdown of FHL3 partially rescued the inhibitory effects of miR-663a inhibitor on melanoma cell behaviors. Together, our work provided evidence that miR-663a functions as an oncogenic miRNA in melanoma. SAGE Publications 2020-10-01 /pmc/articles/PMC7533922/ /pubmed/33000682 http://dx.doi.org/10.1177/1533033820957000 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Liu, Saijun
Hu, Yunfeng
Wu, Shi
He, Yong
Deng, Liehua
MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3
title MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3
title_full MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3
title_fullStr MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3
title_full_unstemmed MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3
title_short MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3
title_sort microrna-663 regulates melanoma progression by inhibiting fhl3
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533922/
https://www.ncbi.nlm.nih.gov/pubmed/33000682
http://dx.doi.org/10.1177/1533033820957000
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