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MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3
microRNA-663a (miR-663a) was reported to be highly expressed in cancers. However, its roles in melanoma progression remain unclear. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted to measure miR-663a expression level in melanoma cell lines and normal cells. Cell...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533922/ https://www.ncbi.nlm.nih.gov/pubmed/33000682 http://dx.doi.org/10.1177/1533033820957000 |
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author | Liu, Saijun Hu, Yunfeng Wu, Shi He, Yong Deng, Liehua |
author_facet | Liu, Saijun Hu, Yunfeng Wu, Shi He, Yong Deng, Liehua |
author_sort | Liu, Saijun |
collection | PubMed |
description | microRNA-663a (miR-663a) was reported to be highly expressed in cancers. However, its roles in melanoma progression remain unclear. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted to measure miR-663a expression level in melanoma cell lines and normal cells. Cell counting kit-8 assay, wound-healing assay, and transwell invasion assay were conducted to analyze biological roles of miR-663a in melanoma. Luciferase activity reporter assay was conducted to validate the connection of miR-663a and Four and a half LIM domain (FHL) protein 3 (FHL3) in melanoma. Our results showed miR-663a expression level was significantly increased in melanoma cells compared with normal cells. Silencing miR-663a expression suppresses melanoma cell proliferation, migration, and invasion in vitro. Moreover, FHL3 was validated as a functional target of miR-663a. Knockdown of FHL3 partially rescued the inhibitory effects of miR-663a inhibitor on melanoma cell behaviors. Together, our work provided evidence that miR-663a functions as an oncogenic miRNA in melanoma. |
format | Online Article Text |
id | pubmed-7533922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-75339222020-10-14 MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3 Liu, Saijun Hu, Yunfeng Wu, Shi He, Yong Deng, Liehua Technol Cancer Res Treat Original Article microRNA-663a (miR-663a) was reported to be highly expressed in cancers. However, its roles in melanoma progression remain unclear. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted to measure miR-663a expression level in melanoma cell lines and normal cells. Cell counting kit-8 assay, wound-healing assay, and transwell invasion assay were conducted to analyze biological roles of miR-663a in melanoma. Luciferase activity reporter assay was conducted to validate the connection of miR-663a and Four and a half LIM domain (FHL) protein 3 (FHL3) in melanoma. Our results showed miR-663a expression level was significantly increased in melanoma cells compared with normal cells. Silencing miR-663a expression suppresses melanoma cell proliferation, migration, and invasion in vitro. Moreover, FHL3 was validated as a functional target of miR-663a. Knockdown of FHL3 partially rescued the inhibitory effects of miR-663a inhibitor on melanoma cell behaviors. Together, our work provided evidence that miR-663a functions as an oncogenic miRNA in melanoma. SAGE Publications 2020-10-01 /pmc/articles/PMC7533922/ /pubmed/33000682 http://dx.doi.org/10.1177/1533033820957000 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Liu, Saijun Hu, Yunfeng Wu, Shi He, Yong Deng, Liehua MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3 |
title | MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3 |
title_full | MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3 |
title_fullStr | MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3 |
title_full_unstemmed | MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3 |
title_short | MicroRNA-663 Regulates Melanoma Progression by Inhibiting FHL3 |
title_sort | microrna-663 regulates melanoma progression by inhibiting fhl3 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533922/ https://www.ncbi.nlm.nih.gov/pubmed/33000682 http://dx.doi.org/10.1177/1533033820957000 |
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