Cargando…

Dynamics of Staphylococcus aureus Cas9 in DNA target Association and Dissociation

Staphylococcus aureus Cas9 (SaCas9) is an RNA‐guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. Its small size facilitates in vivo delivery for genome editing in various organisms. Herein, using single‐molecule and ensemble approaches, we...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhang, Siqi, Zhang, Qian, Hou, Xi‐Miao, Guo, Lijuan, Wang, Fangzhu, Bi, Lulu, Zhang, Xia, Li, Hai‐Hong, Wen, Fengcai, Xi, Xu‐Guang, Huang, Xingxu, Shen, Bin, Sun, Bo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2020
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534634/ https://www.ncbi.nlm.nih.gov/pubmed/32790142 http://dx.doi.org/10.15252/embr.202050184

Ejemplares similares

The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
por: Zhang, Qian, et al.
Publicado: (2019)

Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer
por: Zhang, Qian, et al.
Publicado: (2021)

In vivo genome editing using Staphylococcus aureus Cas9
por: Ran, F. Ann, et al.
Publicado: (2015)

Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9
por: Zhang, Xiya, et al.
Publicado: (2016)

Staphylococcus aureus Cas9 is a multiple-turnover enzyme
por: Yourik, Paul, et al.
Publicado: (2019)

Using Staphylococcus aureus Cas9 to Expand the Scope of Potential Gene Targets for Genome Editing in Soybean
por: Zhang, Yan, et al.
Publicado: (2022)

Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9
por: Kaya, Hidetaka, et al.
Publicado: (2016)

Structural basis of Staphylococcus aureus Cas9 inhibition by AcrIIA14
por: Liu, Hongnan, et al.
Publicado: (2021)

An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease
por: Teng, Allen C.T., et al.
Publicado: (2022)

Genome modification of CXCR4 by Staphylococcus aureus Cas9 renders cells resistance to HIV-1 infection
por: Wang, Qiankun, et al.
Publicado: (2017)

CRISPR-induced indels and base editing using the Staphylococcus aureus Cas9 in potato
por: Veillet, Florian, et al.
Publicado: (2020)

Human RPA activates BLM’s bidirectional DNA unwinding from a nick
por: Qin, Zhenheng, et al.
Publicado: (2020)

Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applications
por: Friedland, Ari E., et al.
Publicado: (2015)

Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
por: Tycko, Josh, et al.
Publicado: (2018)

A Split Staphylococcus aureus Cas9 as a Compact Genome-Editing Tool in Plants
por: Kaya, Hidetaka, et al.
Publicado: (2017)

Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing
por: Zhang, Linlin, et al.
Publicado: (2016)

Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci
por: Chen, Baohui, et al.
Publicado: (2016)

CRISPR-Cas9 modified bacteriophage for treatment of Staphylococcus aureus induced osteomyelitis and soft tissue infection
por: Cobb, Leah H., et al.
Publicado: (2019)

High-fidelity KKH variant of Staphylococcus aureus Cas9 nucleases with improved base mismatch discrimination
por: Yuen, Chaya T L, et al.
Publicado: (2022)

Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9
por: Ma, Yuanwu, et al.
Publicado: (2014)

Publisher Correction: Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
por: Tycko, Josh, et al.
Publicado: (2018)

Application of CRISPR/Cas9 in Alzheimer’s Disease
por: Lu, Likui, et al.
Publicado: (2021)

Cas12a/Guide RNA-Based Platforms for Rapidly and Accurately Identifying Staphylococcus aureus and Methicillin-Resistant S. aureus
por: Cao, Xiaoying, et al.
Publicado: (2023)

Direct evidence of CRISPR-Cas9-mediated mitochondrial genome editing
por: Bi, Rui, et al.
Publicado: (2022)

A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope
por: Hu, Ziying, et al.
Publicado: (2020)

Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection
por: Penewit, Kelsi, et al.
Publicado: (2018)

Genetic engineering of a temperate phage-based delivery system for CRISPR/Cas9 antimicrobials against Staphylococcus aureus
por: Park, Joo Youn, et al.
Publicado: (2017)

Methods and applications of CRISPR/Cas system for genome editing in stem cells
por: Yang, Guang, et al.
Publicado: (2019)

Comparison of gene disruption induced by cytosine base editing‐mediated iSTOP with CRISPR/Cas9‐mediated frameshift
por: Dang, Lu, et al.
Publicado: (2020)

Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes
por: Watters, Kyle E., et al.
Publicado: (2020)

The convergence of head-on DNA unwinding forks induces helicase oligomerization and activity transition
por: Bi, Lulu, et al.
Publicado: (2022)

Identification and Characterization of the CRISPR/Cas System in Staphylococcus aureus Strains From Diverse Sources
por: Cruz-López, Erick Adrian, et al.
Publicado: (2021)

Methicillin-Resistant Staphylococcus aureus ST9 in Pigs in Thailand
por: Larsen, Jesper, et al.
Publicado: (2012)

9. The Skip Phenomenon in Staphylococcus aureus Bacteremia: Clinical Associations
por: Go, John Raymond U, et al.
Publicado: (2021)

Targeted mutagenesis in soybean using the CRISPR-Cas9 system
por: Sun, Xianjun, et al.
Publicado: (2015)

Intracellular Accumulation of Linezolid and Florfenicol in OptrA-Producing Enterococcus faecalis and Staphylococcus aureus
por: Wang, Yingyu, et al.
Publicado: (2018)

Downregulated Expression of Virulence Factors Induced by Benzyl Isothiocyanate in Staphylococcus Aureus: A Transcriptomic Analysis
por: Wang, Xiaoning, et al.
Publicado: (2019)

Characterization of the Inclusion Complexes of Isothiocyanates with γ-Cyclodextrin for Improvement of Antibacterial Activities against Staphylococcus aureus
por: Liu, Jianan, et al.
Publicado: (2021)

Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociation
por: Wang, Jinglong, et al.
Publicado: (2023)

Production of Human Albumin in Pigs Through CRISPR/Cas9-Mediated Knockin of Human cDNA into Swine Albumin Locus in the Zygotes
por: Peng, Jin, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_od147k115jqh8frlrncpslr0so