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Novel phospho-switch function of delta-catenin in dendrite development

In neurons, dendrites form the major sites of information receipt and integration. It is thus vital that, during development, the dendritic arbor is adequately formed to enable proper neural circuit formation and function. While several known processes shape the arbor, little is known of those that...

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Autores principales: Baumert, Ryan, Ji, Hong, Paulucci-Holthauzen, Adriana, Wolfe, Aaron, Sagum, Cari, Hodgson, Louis, Arikkath, Jyothi, Chen, Xiaojiang, Bedford, Mark T., Waxham, M. Neal, McCrea, Pierre D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534926/
https://www.ncbi.nlm.nih.gov/pubmed/33007084
http://dx.doi.org/10.1083/jcb.201909166
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author Baumert, Ryan
Ji, Hong
Paulucci-Holthauzen, Adriana
Wolfe, Aaron
Sagum, Cari
Hodgson, Louis
Arikkath, Jyothi
Chen, Xiaojiang
Bedford, Mark T.
Waxham, M. Neal
McCrea, Pierre D.
author_facet Baumert, Ryan
Ji, Hong
Paulucci-Holthauzen, Adriana
Wolfe, Aaron
Sagum, Cari
Hodgson, Louis
Arikkath, Jyothi
Chen, Xiaojiang
Bedford, Mark T.
Waxham, M. Neal
McCrea, Pierre D.
author_sort Baumert, Ryan
collection PubMed
description In neurons, dendrites form the major sites of information receipt and integration. It is thus vital that, during development, the dendritic arbor is adequately formed to enable proper neural circuit formation and function. While several known processes shape the arbor, little is known of those that govern dendrite branching versus extension. Here, we report a new mechanism instructing dendrites to branch versus extend. In it, glutamate signaling activates mGluR5 receptors to promote Ckd5-mediated phosphorylation of the C-terminal PDZ-binding motif of delta-catenin. The phosphorylation state of this motif determines delta-catenin’s ability to bind either Pdlim5 or Magi1. Whereas the delta:Pdlim5 complex enhances dendrite branching at the expense of elongation, the delta:Magi1 complex instead promotes lengthening. Our data suggest that these complexes affect dendrite development by differentially regulating the small-GTPase RhoA and actin-associated protein Cortactin. We thus reveal a “phospho-switch” within delta-catenin, subject to a glutamate-mediated signaling pathway, that assists in balancing the branching versus extension of dendrites during neural development.
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spelling pubmed-75349262021-05-02 Novel phospho-switch function of delta-catenin in dendrite development Baumert, Ryan Ji, Hong Paulucci-Holthauzen, Adriana Wolfe, Aaron Sagum, Cari Hodgson, Louis Arikkath, Jyothi Chen, Xiaojiang Bedford, Mark T. Waxham, M. Neal McCrea, Pierre D. J Cell Biol Article In neurons, dendrites form the major sites of information receipt and integration. It is thus vital that, during development, the dendritic arbor is adequately formed to enable proper neural circuit formation and function. While several known processes shape the arbor, little is known of those that govern dendrite branching versus extension. Here, we report a new mechanism instructing dendrites to branch versus extend. In it, glutamate signaling activates mGluR5 receptors to promote Ckd5-mediated phosphorylation of the C-terminal PDZ-binding motif of delta-catenin. The phosphorylation state of this motif determines delta-catenin’s ability to bind either Pdlim5 or Magi1. Whereas the delta:Pdlim5 complex enhances dendrite branching at the expense of elongation, the delta:Magi1 complex instead promotes lengthening. Our data suggest that these complexes affect dendrite development by differentially regulating the small-GTPase RhoA and actin-associated protein Cortactin. We thus reveal a “phospho-switch” within delta-catenin, subject to a glutamate-mediated signaling pathway, that assists in balancing the branching versus extension of dendrites during neural development. Rockefeller University Press 2020-10-01 /pmc/articles/PMC7534926/ /pubmed/33007084 http://dx.doi.org/10.1083/jcb.201909166 Text en © 2020 Baumert et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Baumert, Ryan
Ji, Hong
Paulucci-Holthauzen, Adriana
Wolfe, Aaron
Sagum, Cari
Hodgson, Louis
Arikkath, Jyothi
Chen, Xiaojiang
Bedford, Mark T.
Waxham, M. Neal
McCrea, Pierre D.
Novel phospho-switch function of delta-catenin in dendrite development
title Novel phospho-switch function of delta-catenin in dendrite development
title_full Novel phospho-switch function of delta-catenin in dendrite development
title_fullStr Novel phospho-switch function of delta-catenin in dendrite development
title_full_unstemmed Novel phospho-switch function of delta-catenin in dendrite development
title_short Novel phospho-switch function of delta-catenin in dendrite development
title_sort novel phospho-switch function of delta-catenin in dendrite development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534926/
https://www.ncbi.nlm.nih.gov/pubmed/33007084
http://dx.doi.org/10.1083/jcb.201909166
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