The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence

INTRODUCTION: Gene-Xpert MTB RIF (Xpert) is based on nucleic acid amplification by real-time polymerase chain reaction, which allows for the identification of Mycobacterium tuberculosis and rifampin resistance. We describe the use of Xpert for extrapulmonary tuberculosis (EPTB) in children and adole...

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Autores principales: Aurilio, Rafaela Baroni, Marsili, Vivian Vidal, Malaquias, Thiago da Silva Santos, Kritski, Afrânio Lineu, Sant’Anna, Clemax Couto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534970/
https://www.ncbi.nlm.nih.gov/pubmed/33027414
http://dx.doi.org/10.1590/0037-8682-0104-2020
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author Aurilio, Rafaela Baroni
Marsili, Vivian Vidal
Malaquias, Thiago da Silva Santos
Kritski, Afrânio Lineu
Sant’Anna, Clemax Couto
author_facet Aurilio, Rafaela Baroni
Marsili, Vivian Vidal
Malaquias, Thiago da Silva Santos
Kritski, Afrânio Lineu
Sant’Anna, Clemax Couto
author_sort Aurilio, Rafaela Baroni
collection PubMed
description INTRODUCTION: Gene-Xpert MTB RIF (Xpert) is based on nucleic acid amplification by real-time polymerase chain reaction, which allows for the identification of Mycobacterium tuberculosis and rifampin resistance. We describe the use of Xpert for extrapulmonary tuberculosis (EPTB) in children and adolescents. METHODS: A case series of two reference centers in Rio de Janeiro from 2014-2019. RESULTS: The final diagnosis of EPTB was established in 11/36 (31%) patients, with five cases detectable by Xpert. For lymph node evaluation (9/11), diagnosis by Xpert occurred in 5/9 patients, all with caseous aspects. CONCLUSIONS: Xpert can facilitate the rapid diagnosis of lymph node tuberculosis.
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spelling pubmed-75349702020-10-06 The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence Aurilio, Rafaela Baroni Marsili, Vivian Vidal Malaquias, Thiago da Silva Santos Kritski, Afrânio Lineu Sant’Anna, Clemax Couto Rev Soc Bras Med Trop Short Communication INTRODUCTION: Gene-Xpert MTB RIF (Xpert) is based on nucleic acid amplification by real-time polymerase chain reaction, which allows for the identification of Mycobacterium tuberculosis and rifampin resistance. We describe the use of Xpert for extrapulmonary tuberculosis (EPTB) in children and adolescents. METHODS: A case series of two reference centers in Rio de Janeiro from 2014-2019. RESULTS: The final diagnosis of EPTB was established in 11/36 (31%) patients, with five cases detectable by Xpert. For lymph node evaluation (9/11), diagnosis by Xpert occurred in 5/9 patients, all with caseous aspects. CONCLUSIONS: Xpert can facilitate the rapid diagnosis of lymph node tuberculosis. Sociedade Brasileira de Medicina Tropical - SBMT 2020-10-05 /pmc/articles/PMC7534970/ /pubmed/33027414 http://dx.doi.org/10.1590/0037-8682-0104-2020 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Aurilio, Rafaela Baroni
Marsili, Vivian Vidal
Malaquias, Thiago da Silva Santos
Kritski, Afrânio Lineu
Sant’Anna, Clemax Couto
The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence
title The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence
title_full The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence
title_fullStr The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence
title_full_unstemmed The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence
title_short The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence
title_sort use of gene-xpert mtb rif in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534970/
https://www.ncbi.nlm.nih.gov/pubmed/33027414
http://dx.doi.org/10.1590/0037-8682-0104-2020
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