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Fast and antibiotic free genome integration into Escherichia coli chromosome

Genome-based Escherichia coli expression systems are superior to conventional plasmid-based systems as the metabolic load triggered by recombinant compounds is significantly reduced. The efficiency of T7-based transcription compensates for low gene dosage (single copy) and facilitates high product f...

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Detalles Bibliográficos
Autores principales:	Egger, Esther, Tauer, Christopher, Cserjan-Puschmann, Monika, Grabherr, Reingard, Striedner, Gerald
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536200/ https://www.ncbi.nlm.nih.gov/pubmed/33020519 http://dx.doi.org/10.1038/s41598-020-73348-x

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