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A Purkinje image-based system for an assessment of the density and transmittance spectra of the human crystalline lens in vivo
A method for rapid and objective assessment of ocular lens density and transmittance is needed for research and clinical practice. The aim of this study was to determine whether the Purkinje image-based technique can be used for objective and accurate quantification of spectral density and transmitt...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536217/ https://www.ncbi.nlm.nih.gov/pubmed/33020575 http://dx.doi.org/10.1038/s41598-020-73541-y |
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author | Eto, Taisuke Teikari, Petteri Najjar, Raymond P. Nishimura, Yuki Motomura, Yuki Kuze, Manami Higuchi, Shigekazu |
author_facet | Eto, Taisuke Teikari, Petteri Najjar, Raymond P. Nishimura, Yuki Motomura, Yuki Kuze, Manami Higuchi, Shigekazu |
author_sort | Eto, Taisuke |
collection | PubMed |
description | A method for rapid and objective assessment of ocular lens density and transmittance is needed for research and clinical practice. The aim of this study was to determine whether the Purkinje image-based technique can be used for objective and accurate quantification of spectral density and transmittance of ocular media (the mainly crystalline lens) in visible light. Twenty-six individuals (10 young, 9 middle-aged and 7 older individuals) participated in this study. Spectral lens density was evaluated by detecting the intensity of the IVth Purkinje image for different wavelengths. Subsequently, optical density index (ODI), the area under the curve in the lens density spectrum, was calculated and ODIs were compared with clinical lens opacification scales assessed subjectively using a slit lamp. Spectral lens transmittance was estimated from the lens density spectrum. Lens densities were higher in the short wavelength region of the visible spectrum across all age groups. ODI was highly correlated with the clinical opacification scale, while lens transmittance decreased with aging. Our results showed that spectral transmittance of the human crystalline lens can be easily estimated from optical density spectra evaluated objectively and rapidly using the Purkinje image-based technique. Our results provide clinicians and scientists with an accurate, rapid and objective technique for quantification of lens transmittance. |
format | Online Article Text |
id | pubmed-7536217 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75362172020-10-07 A Purkinje image-based system for an assessment of the density and transmittance spectra of the human crystalline lens in vivo Eto, Taisuke Teikari, Petteri Najjar, Raymond P. Nishimura, Yuki Motomura, Yuki Kuze, Manami Higuchi, Shigekazu Sci Rep Article A method for rapid and objective assessment of ocular lens density and transmittance is needed for research and clinical practice. The aim of this study was to determine whether the Purkinje image-based technique can be used for objective and accurate quantification of spectral density and transmittance of ocular media (the mainly crystalline lens) in visible light. Twenty-six individuals (10 young, 9 middle-aged and 7 older individuals) participated in this study. Spectral lens density was evaluated by detecting the intensity of the IVth Purkinje image for different wavelengths. Subsequently, optical density index (ODI), the area under the curve in the lens density spectrum, was calculated and ODIs were compared with clinical lens opacification scales assessed subjectively using a slit lamp. Spectral lens transmittance was estimated from the lens density spectrum. Lens densities were higher in the short wavelength region of the visible spectrum across all age groups. ODI was highly correlated with the clinical opacification scale, while lens transmittance decreased with aging. Our results showed that spectral transmittance of the human crystalline lens can be easily estimated from optical density spectra evaluated objectively and rapidly using the Purkinje image-based technique. Our results provide clinicians and scientists with an accurate, rapid and objective technique for quantification of lens transmittance. Nature Publishing Group UK 2020-10-05 /pmc/articles/PMC7536217/ /pubmed/33020575 http://dx.doi.org/10.1038/s41598-020-73541-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Eto, Taisuke Teikari, Petteri Najjar, Raymond P. Nishimura, Yuki Motomura, Yuki Kuze, Manami Higuchi, Shigekazu A Purkinje image-based system for an assessment of the density and transmittance spectra of the human crystalline lens in vivo |
title | A Purkinje image-based system for an assessment of the density and transmittance spectra of the human crystalline lens in vivo |
title_full | A Purkinje image-based system for an assessment of the density and transmittance spectra of the human crystalline lens in vivo |
title_fullStr | A Purkinje image-based system for an assessment of the density and transmittance spectra of the human crystalline lens in vivo |
title_full_unstemmed | A Purkinje image-based system for an assessment of the density and transmittance spectra of the human crystalline lens in vivo |
title_short | A Purkinje image-based system for an assessment of the density and transmittance spectra of the human crystalline lens in vivo |
title_sort | purkinje image-based system for an assessment of the density and transmittance spectra of the human crystalline lens in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536217/ https://www.ncbi.nlm.nih.gov/pubmed/33020575 http://dx.doi.org/10.1038/s41598-020-73541-y |
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