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Neuroprotective effect of apigenin against cerebral ischemia/reperfusion injury
OBJECTIVE: The therapeutic efficacy of apigenin in PC12 cells and rats remains uncertain. The aim of this study was to investigate the neuroprotective effects of apigenin against cerebral ischemia/reperfusion injury, both in vitro and in vivo. METHODS: We first treated PC12 cells with cobalt chlorid...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536502/ https://www.ncbi.nlm.nih.gov/pubmed/32993408 http://dx.doi.org/10.1177/0300060520945859 |
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author | Ling, Chengli Lei, Chang Zou, Manshu Cai, Xiong Xiang, Yun Xie, Yu Li, Xuran Huang, Dan Wang, Yuhong |
author_facet | Ling, Chengli Lei, Chang Zou, Manshu Cai, Xiong Xiang, Yun Xie, Yu Li, Xuran Huang, Dan Wang, Yuhong |
author_sort | Ling, Chengli |
collection | PubMed |
description | OBJECTIVE: The therapeutic efficacy of apigenin in PC12 cells and rats remains uncertain. The aim of this study was to investigate the neuroprotective effects of apigenin against cerebral ischemia/reperfusion injury, both in vitro and in vivo. METHODS: We first treated PC12 cells with cobalt chloride (CoCl(2)) to create a model of oxidative stress injury. Cell viability was then determined using a multifunctional microplate reader. In addition, reactive oxygen species (ROS) levels, apoptosis, and mitochondrial membrane potentials (MMPs) were examined using high-content cytometer analysis. The efficacy of apigenin treatment was also analyzed in a rat middle cerebral artery occlusion (MCAO) model using TTC staining and neurological deficit scores. RESULTS: The half-inhibitory concentration of CoCl(2) was 1.2 mM. Pretreatment with 10 µg ⋅ mL(−1) apigenin significantly enhanced cell viability, reduced ROS levels, alleviated apoptosis, and improved MMP in PC12 cells with CoCl(2)-induced injury in vitro. In addition, apigenin treatment in vivo significantly improved neurological deficit scores and reduced infarct areas in MCAO rats. These results suggest that the neuroprotective mechanisms of apigenin may be related to mitochondrial activation. CONCLUSIONS: Apigenin had excellent neuroprotective effects for the treatment of cerebral ischemia/reperfusion injury in vitro and in vivo. |
format | Online Article Text |
id | pubmed-7536502 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-75365022020-10-15 Neuroprotective effect of apigenin against cerebral ischemia/reperfusion injury Ling, Chengli Lei, Chang Zou, Manshu Cai, Xiong Xiang, Yun Xie, Yu Li, Xuran Huang, Dan Wang, Yuhong J Int Med Res Pre-Clinical Research Report OBJECTIVE: The therapeutic efficacy of apigenin in PC12 cells and rats remains uncertain. The aim of this study was to investigate the neuroprotective effects of apigenin against cerebral ischemia/reperfusion injury, both in vitro and in vivo. METHODS: We first treated PC12 cells with cobalt chloride (CoCl(2)) to create a model of oxidative stress injury. Cell viability was then determined using a multifunctional microplate reader. In addition, reactive oxygen species (ROS) levels, apoptosis, and mitochondrial membrane potentials (MMPs) were examined using high-content cytometer analysis. The efficacy of apigenin treatment was also analyzed in a rat middle cerebral artery occlusion (MCAO) model using TTC staining and neurological deficit scores. RESULTS: The half-inhibitory concentration of CoCl(2) was 1.2 mM. Pretreatment with 10 µg ⋅ mL(−1) apigenin significantly enhanced cell viability, reduced ROS levels, alleviated apoptosis, and improved MMP in PC12 cells with CoCl(2)-induced injury in vitro. In addition, apigenin treatment in vivo significantly improved neurological deficit scores and reduced infarct areas in MCAO rats. These results suggest that the neuroprotective mechanisms of apigenin may be related to mitochondrial activation. CONCLUSIONS: Apigenin had excellent neuroprotective effects for the treatment of cerebral ischemia/reperfusion injury in vitro and in vivo. SAGE Publications 2020-09-29 /pmc/articles/PMC7536502/ /pubmed/32993408 http://dx.doi.org/10.1177/0300060520945859 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Pre-Clinical Research Report Ling, Chengli Lei, Chang Zou, Manshu Cai, Xiong Xiang, Yun Xie, Yu Li, Xuran Huang, Dan Wang, Yuhong Neuroprotective effect of apigenin against cerebral ischemia/reperfusion injury |
title | Neuroprotective effect of apigenin against cerebral
ischemia/reperfusion injury |
title_full | Neuroprotective effect of apigenin against cerebral
ischemia/reperfusion injury |
title_fullStr | Neuroprotective effect of apigenin against cerebral
ischemia/reperfusion injury |
title_full_unstemmed | Neuroprotective effect of apigenin against cerebral
ischemia/reperfusion injury |
title_short | Neuroprotective effect of apigenin against cerebral
ischemia/reperfusion injury |
title_sort | neuroprotective effect of apigenin against cerebral
ischemia/reperfusion injury |
topic | Pre-Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536502/ https://www.ncbi.nlm.nih.gov/pubmed/32993408 http://dx.doi.org/10.1177/0300060520945859 |
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