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Potent human broadly neutralizing antibodies to hepatitis B virus from natural controllers
Rare individuals can naturally clear chronic hepatitis B virus (HBV) infection and acquire protection from reinfection as conferred by vaccination. To examine the protective humoral response against HBV, we cloned and characterized human antibodies specific to the viral surface glycoproteins (HBsAg)...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537403/ https://www.ncbi.nlm.nih.gov/pubmed/32579155 http://dx.doi.org/10.1084/jem.20200840 |
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author | Hehle, Verena Beretta, Maxime Bourgine, Maryline Ait-Goughoulte, Malika Planchais, Cyril Morisse, Solen Vesin, Benjamin Lorin, Valérie Hieu, Thierry Stauffer, Andrea Fiquet, Oriane Dimitrov, Jordan D. Michel, Marie-Louise Ungeheuer, Marie-Noëlle Sureau, Camille Pol, Stanislas Di Santo, James P. Strick-Marchand, Hélène Pelletier, Nadège Mouquet, Hugo |
author_facet | Hehle, Verena Beretta, Maxime Bourgine, Maryline Ait-Goughoulte, Malika Planchais, Cyril Morisse, Solen Vesin, Benjamin Lorin, Valérie Hieu, Thierry Stauffer, Andrea Fiquet, Oriane Dimitrov, Jordan D. Michel, Marie-Louise Ungeheuer, Marie-Noëlle Sureau, Camille Pol, Stanislas Di Santo, James P. Strick-Marchand, Hélène Pelletier, Nadège Mouquet, Hugo |
author_sort | Hehle, Verena |
collection | PubMed |
description | Rare individuals can naturally clear chronic hepatitis B virus (HBV) infection and acquire protection from reinfection as conferred by vaccination. To examine the protective humoral response against HBV, we cloned and characterized human antibodies specific to the viral surface glycoproteins (HBsAg) from memory B cells of HBV vaccinees and controllers. We found that human HBV antibodies are encoded by a diverse set of immunoglobulin genes and recognize various conformational HBsAg epitopes. Strikingly, HBsAg-specific memory B cells from natural controllers mainly produced neutralizing antibodies able to cross-react with several viral genotypes. Furthermore, monotherapy with the potent broadly neutralizing antibody Bc1.187 suppressed viremia in vivo in HBV mouse models and led to post-therapy control of the infection in a fraction of animals. Thus, human neutralizing HBsAg antibodies appear to play a key role in the spontaneous control of HBV and represent promising immunotherapeutic tools for achieving HBV functional cure in chronically infected humans. |
format | Online Article Text |
id | pubmed-7537403 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-75374032021-04-05 Potent human broadly neutralizing antibodies to hepatitis B virus from natural controllers Hehle, Verena Beretta, Maxime Bourgine, Maryline Ait-Goughoulte, Malika Planchais, Cyril Morisse, Solen Vesin, Benjamin Lorin, Valérie Hieu, Thierry Stauffer, Andrea Fiquet, Oriane Dimitrov, Jordan D. Michel, Marie-Louise Ungeheuer, Marie-Noëlle Sureau, Camille Pol, Stanislas Di Santo, James P. Strick-Marchand, Hélène Pelletier, Nadège Mouquet, Hugo J Exp Med Article Rare individuals can naturally clear chronic hepatitis B virus (HBV) infection and acquire protection from reinfection as conferred by vaccination. To examine the protective humoral response against HBV, we cloned and characterized human antibodies specific to the viral surface glycoproteins (HBsAg) from memory B cells of HBV vaccinees and controllers. We found that human HBV antibodies are encoded by a diverse set of immunoglobulin genes and recognize various conformational HBsAg epitopes. Strikingly, HBsAg-specific memory B cells from natural controllers mainly produced neutralizing antibodies able to cross-react with several viral genotypes. Furthermore, monotherapy with the potent broadly neutralizing antibody Bc1.187 suppressed viremia in vivo in HBV mouse models and led to post-therapy control of the infection in a fraction of animals. Thus, human neutralizing HBsAg antibodies appear to play a key role in the spontaneous control of HBV and represent promising immunotherapeutic tools for achieving HBV functional cure in chronically infected humans. Rockefeller University Press 2020-06-24 /pmc/articles/PMC7537403/ /pubmed/32579155 http://dx.doi.org/10.1084/jem.20200840 Text en © 2020 Hehle et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Hehle, Verena Beretta, Maxime Bourgine, Maryline Ait-Goughoulte, Malika Planchais, Cyril Morisse, Solen Vesin, Benjamin Lorin, Valérie Hieu, Thierry Stauffer, Andrea Fiquet, Oriane Dimitrov, Jordan D. Michel, Marie-Louise Ungeheuer, Marie-Noëlle Sureau, Camille Pol, Stanislas Di Santo, James P. Strick-Marchand, Hélène Pelletier, Nadège Mouquet, Hugo Potent human broadly neutralizing antibodies to hepatitis B virus from natural controllers |
title | Potent human broadly neutralizing antibodies to hepatitis B virus from natural controllers |
title_full | Potent human broadly neutralizing antibodies to hepatitis B virus from natural controllers |
title_fullStr | Potent human broadly neutralizing antibodies to hepatitis B virus from natural controllers |
title_full_unstemmed | Potent human broadly neutralizing antibodies to hepatitis B virus from natural controllers |
title_short | Potent human broadly neutralizing antibodies to hepatitis B virus from natural controllers |
title_sort | potent human broadly neutralizing antibodies to hepatitis b virus from natural controllers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537403/ https://www.ncbi.nlm.nih.gov/pubmed/32579155 http://dx.doi.org/10.1084/jem.20200840 |
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