Cargando…

Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice

The oral lipid tolerance test (OLTT) has been known to assess intestinal fat metabolism and whole-body lipid metabolism, but rodent models for OLTT are not yet established. Differences in OLTT methodology preclude the generation of definitive results, which may cause some confusion about the anti-hy...

Descripción completa

Detalles Bibliográficos
Autor principal: Ochiai, Masaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537863/
https://www.ncbi.nlm.nih.gov/pubmed/33022003
http://dx.doi.org/10.1371/journal.pone.0235875
_version_ 1783590749557751808
author Ochiai, Masaru
author_facet Ochiai, Masaru
author_sort Ochiai, Masaru
collection PubMed
description The oral lipid tolerance test (OLTT) has been known to assess intestinal fat metabolism and whole-body lipid metabolism, but rodent models for OLTT are not yet established. Differences in OLTT methodology preclude the generation of definitive results, which may cause some confusion about the anti-hypertriglyceridemia effects of the test materials. To standardize and generate more appropriate methodology for the OLTT, we examined the effects of mice strain, dietary lipid sources, fasting period, and gender on lipid-induced hypertriglyceridemia in mice. First, lipid-induced hypertriglyceridemia was more strongly observed in male ddY mice than in C57BL/6N or ICR mice. Second, the administration of olive and soybean oils remarkably represented lipid-induced hypertriglyceridemia. Third, fasting period before the OLTT largely affected the plasma triglyceride elevation. Fasting for 12 h, but less than 48 h, provoked lipid-induced hypertriglyceridemia. Fourth, we explored the suppressive effects of epigallocatechin gallate (EGCG), a green tea polyphenol, on lipid-induced hypertriglyceridemia. The administration of 100 mg/kg of EGCG suppressed lipid-induced hypertriglyceridemia and intestinal lipase activity. Fifth, EGCG-induced suppressive effects were observed after lipid-induced hypertriglyceridemia was observed in male mice, but not in female mice. Lastly, lipid-induced hypertriglyceridemia could be more effectively induced in mice fed a high-fat diet for 1 week before the OLTT. These findings indicate that male ddY mice after 12 h fasting displayed marked lipid-induced hypertriglyceridemia in response to soybean oil. Hence, the defined experiment condition may be a more appropriate OLTT model for evaluating lipid-induced hypertriglyceridemia.
format Online
Article
Text
id pubmed-7537863
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-75378632020-10-15 Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice Ochiai, Masaru PLoS One Research Article The oral lipid tolerance test (OLTT) has been known to assess intestinal fat metabolism and whole-body lipid metabolism, but rodent models for OLTT are not yet established. Differences in OLTT methodology preclude the generation of definitive results, which may cause some confusion about the anti-hypertriglyceridemia effects of the test materials. To standardize and generate more appropriate methodology for the OLTT, we examined the effects of mice strain, dietary lipid sources, fasting period, and gender on lipid-induced hypertriglyceridemia in mice. First, lipid-induced hypertriglyceridemia was more strongly observed in male ddY mice than in C57BL/6N or ICR mice. Second, the administration of olive and soybean oils remarkably represented lipid-induced hypertriglyceridemia. Third, fasting period before the OLTT largely affected the plasma triglyceride elevation. Fasting for 12 h, but less than 48 h, provoked lipid-induced hypertriglyceridemia. Fourth, we explored the suppressive effects of epigallocatechin gallate (EGCG), a green tea polyphenol, on lipid-induced hypertriglyceridemia. The administration of 100 mg/kg of EGCG suppressed lipid-induced hypertriglyceridemia and intestinal lipase activity. Fifth, EGCG-induced suppressive effects were observed after lipid-induced hypertriglyceridemia was observed in male mice, but not in female mice. Lastly, lipid-induced hypertriglyceridemia could be more effectively induced in mice fed a high-fat diet for 1 week before the OLTT. These findings indicate that male ddY mice after 12 h fasting displayed marked lipid-induced hypertriglyceridemia in response to soybean oil. Hence, the defined experiment condition may be a more appropriate OLTT model for evaluating lipid-induced hypertriglyceridemia. Public Library of Science 2020-10-06 /pmc/articles/PMC7537863/ /pubmed/33022003 http://dx.doi.org/10.1371/journal.pone.0235875 Text en © 2020 Masaru Ochiai http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ochiai, Masaru
Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice
title Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice
title_full Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice
title_fullStr Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice
title_full_unstemmed Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice
title_short Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice
title_sort evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537863/
https://www.ncbi.nlm.nih.gov/pubmed/33022003
http://dx.doi.org/10.1371/journal.pone.0235875
work_keys_str_mv AT ochiaimasaru evaluatingtheappropriateorallipidtolerancetestmodelforinvestigatingplasmatriglycerideelevationinmice