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Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina
An important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces of neuroepithelia during interkinetic nuclear migration. Tracking of nuclear subpopulations has shown evidence of diffusion - mean squared displacements growing linearly i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538155/ https://www.ncbi.nlm.nih.gov/pubmed/33021471 http://dx.doi.org/10.7554/eLife.58635 |
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author | Azizi, Afnan Herrmann, Anne Wan, Yinan Buse, Salvador JRP Keller, Philipp J Goldstein, Raymond E Harris, William A |
author_facet | Azizi, Afnan Herrmann, Anne Wan, Yinan Buse, Salvador JRP Keller, Philipp J Goldstein, Raymond E Harris, William A |
author_sort | Azizi, Afnan |
collection | PubMed |
description | An important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces of neuroepithelia during interkinetic nuclear migration. Tracking of nuclear subpopulations has shown evidence of diffusion - mean squared displacements growing linearly in time - and suggested crowding from cell division at the apical surface drives basalward motion. Yet, this hypothesis has not yet been tested, and the forces involved not quantified. We employ long-term, rapid light-sheet and two-photon imaging of early zebrafish retinogenesis to track entire populations of nuclei within the tissue. The time-varying concentration profiles show clear evidence of crowding as nuclei reach close-packing and are quantitatively described by a nonlinear diffusion model. Considerations of nuclear motion constrained inside the enveloping cell membrane show that concentration-dependent stochastic forces inside cells, compatible in magnitude to those found in cytoskeletal transport, can explain the observed magnitude of the diffusion constant. |
format | Online Article Text |
id | pubmed-7538155 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-75381552020-10-07 Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina Azizi, Afnan Herrmann, Anne Wan, Yinan Buse, Salvador JRP Keller, Philipp J Goldstein, Raymond E Harris, William A eLife Developmental Biology An important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces of neuroepithelia during interkinetic nuclear migration. Tracking of nuclear subpopulations has shown evidence of diffusion - mean squared displacements growing linearly in time - and suggested crowding from cell division at the apical surface drives basalward motion. Yet, this hypothesis has not yet been tested, and the forces involved not quantified. We employ long-term, rapid light-sheet and two-photon imaging of early zebrafish retinogenesis to track entire populations of nuclei within the tissue. The time-varying concentration profiles show clear evidence of crowding as nuclei reach close-packing and are quantitatively described by a nonlinear diffusion model. Considerations of nuclear motion constrained inside the enveloping cell membrane show that concentration-dependent stochastic forces inside cells, compatible in magnitude to those found in cytoskeletal transport, can explain the observed magnitude of the diffusion constant. eLife Sciences Publications, Ltd 2020-10-06 /pmc/articles/PMC7538155/ /pubmed/33021471 http://dx.doi.org/10.7554/eLife.58635 Text en © 2020, Azizi et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Azizi, Afnan Herrmann, Anne Wan, Yinan Buse, Salvador JRP Keller, Philipp J Goldstein, Raymond E Harris, William A Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title | Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title_full | Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title_fullStr | Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title_full_unstemmed | Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title_short | Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title_sort | nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538155/ https://www.ncbi.nlm.nih.gov/pubmed/33021471 http://dx.doi.org/10.7554/eLife.58635 |
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