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Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina

An important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces of neuroepithelia during interkinetic nuclear migration. Tracking of nuclear subpopulations has shown evidence of diffusion - mean squared displacements growing linearly i...

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Autores principales: Azizi, Afnan, Herrmann, Anne, Wan, Yinan, Buse, Salvador JRP, Keller, Philipp J, Goldstein, Raymond E, Harris, William A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538155/
https://www.ncbi.nlm.nih.gov/pubmed/33021471
http://dx.doi.org/10.7554/eLife.58635
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author Azizi, Afnan
Herrmann, Anne
Wan, Yinan
Buse, Salvador JRP
Keller, Philipp J
Goldstein, Raymond E
Harris, William A
author_facet Azizi, Afnan
Herrmann, Anne
Wan, Yinan
Buse, Salvador JRP
Keller, Philipp J
Goldstein, Raymond E
Harris, William A
author_sort Azizi, Afnan
collection PubMed
description An important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces of neuroepithelia during interkinetic nuclear migration. Tracking of nuclear subpopulations has shown evidence of diffusion - mean squared displacements growing linearly in time - and suggested crowding from cell division at the apical surface drives basalward motion. Yet, this hypothesis has not yet been tested, and the forces involved not quantified. We employ long-term, rapid light-sheet and two-photon imaging of early zebrafish retinogenesis to track entire populations of nuclei within the tissue. The time-varying concentration profiles show clear evidence of crowding as nuclei reach close-packing and are quantitatively described by a nonlinear diffusion model. Considerations of nuclear motion constrained inside the enveloping cell membrane show that concentration-dependent stochastic forces inside cells, compatible in magnitude to those found in cytoskeletal transport, can explain the observed magnitude of the diffusion constant.
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spelling pubmed-75381552020-10-07 Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina Azizi, Afnan Herrmann, Anne Wan, Yinan Buse, Salvador JRP Keller, Philipp J Goldstein, Raymond E Harris, William A eLife Developmental Biology An important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces of neuroepithelia during interkinetic nuclear migration. Tracking of nuclear subpopulations has shown evidence of diffusion - mean squared displacements growing linearly in time - and suggested crowding from cell division at the apical surface drives basalward motion. Yet, this hypothesis has not yet been tested, and the forces involved not quantified. We employ long-term, rapid light-sheet and two-photon imaging of early zebrafish retinogenesis to track entire populations of nuclei within the tissue. The time-varying concentration profiles show clear evidence of crowding as nuclei reach close-packing and are quantitatively described by a nonlinear diffusion model. Considerations of nuclear motion constrained inside the enveloping cell membrane show that concentration-dependent stochastic forces inside cells, compatible in magnitude to those found in cytoskeletal transport, can explain the observed magnitude of the diffusion constant. eLife Sciences Publications, Ltd 2020-10-06 /pmc/articles/PMC7538155/ /pubmed/33021471 http://dx.doi.org/10.7554/eLife.58635 Text en © 2020, Azizi et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Azizi, Afnan
Herrmann, Anne
Wan, Yinan
Buse, Salvador JRP
Keller, Philipp J
Goldstein, Raymond E
Harris, William A
Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina
title Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina
title_full Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina
title_fullStr Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina
title_full_unstemmed Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina
title_short Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina
title_sort nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538155/
https://www.ncbi.nlm.nih.gov/pubmed/33021471
http://dx.doi.org/10.7554/eLife.58635
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