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Cascading effects in freshwater microbial food webs by predatory Cercozoa, Katablepharidacea and ciliates feeding on aplastidic bacterivorous cryptophytes

Heterotrophic nanoflagellates (HNF) are considered as major planktonic bacterivores, however, larger HNF taxa can also be important predators of eukaryotes. To examine this trophic cascading, natural protistan communities from a freshwater reservoir were released from grazing pressure by zooplankton...

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Autores principales: Šimek, Karel, Grujčić, Vesna, Mukherjee, Indranil, Kasalický, Vojtěch, Nedoma, Jiří, Posch, Thomas, Mehrshad, Maliheh, Salcher, Michaela M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538307/
https://www.ncbi.nlm.nih.gov/pubmed/32556274
http://dx.doi.org/10.1093/femsec/fiaa121
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author Šimek, Karel
Grujčić, Vesna
Mukherjee, Indranil
Kasalický, Vojtěch
Nedoma, Jiří
Posch, Thomas
Mehrshad, Maliheh
Salcher, Michaela M
author_facet Šimek, Karel
Grujčić, Vesna
Mukherjee, Indranil
Kasalický, Vojtěch
Nedoma, Jiří
Posch, Thomas
Mehrshad, Maliheh
Salcher, Michaela M
author_sort Šimek, Karel
collection PubMed
description Heterotrophic nanoflagellates (HNF) are considered as major planktonic bacterivores, however, larger HNF taxa can also be important predators of eukaryotes. To examine this trophic cascading, natural protistan communities from a freshwater reservoir were released from grazing pressure by zooplankton via filtration through 10- and 5-µm filters, yielding microbial food webs of different complexity. Protistan growth was stimulated by amendments of five Limnohabitans strains, thus yielding five prey-specific treatments distinctly modulating protistan communities in 10- versus 5-µm fractions. HNF dynamics was tracked by applying five eukaryotic fluorescence in situ hybridization probes covering 55–90% of total flagellates. During the first experimental part, mainly small bacterivorous Cryptophyceae prevailed, with significantly higher abundances in 5-µm treatments. Larger predatory flagellates affiliating with Katablepharidacea and one Cercozoan lineage (increasing to up to 28% of total HNF) proliferated towards the experimental endpoint, having obviously small phagocytized HNF in their food vacuoles. These predatory flagellates reached higher abundances in 10-µm treatments, where small ciliate predators and flagellate hunters also (Urotricha spp., Balanion planctonicum) dominated the ciliate assemblage. Overall, our study reports pronounced cascading effects from bacteria to bacterivorous HNF, predatory HNF and ciliates in highly treatment-specific fashions, defined by both prey-food characteristics and feeding modes of predominating protists.
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spelling pubmed-75383072020-10-13 Cascading effects in freshwater microbial food webs by predatory Cercozoa, Katablepharidacea and ciliates feeding on aplastidic bacterivorous cryptophytes Šimek, Karel Grujčić, Vesna Mukherjee, Indranil Kasalický, Vojtěch Nedoma, Jiří Posch, Thomas Mehrshad, Maliheh Salcher, Michaela M FEMS Microbiol Ecol Research Article Heterotrophic nanoflagellates (HNF) are considered as major planktonic bacterivores, however, larger HNF taxa can also be important predators of eukaryotes. To examine this trophic cascading, natural protistan communities from a freshwater reservoir were released from grazing pressure by zooplankton via filtration through 10- and 5-µm filters, yielding microbial food webs of different complexity. Protistan growth was stimulated by amendments of five Limnohabitans strains, thus yielding five prey-specific treatments distinctly modulating protistan communities in 10- versus 5-µm fractions. HNF dynamics was tracked by applying five eukaryotic fluorescence in situ hybridization probes covering 55–90% of total flagellates. During the first experimental part, mainly small bacterivorous Cryptophyceae prevailed, with significantly higher abundances in 5-µm treatments. Larger predatory flagellates affiliating with Katablepharidacea and one Cercozoan lineage (increasing to up to 28% of total HNF) proliferated towards the experimental endpoint, having obviously small phagocytized HNF in their food vacuoles. These predatory flagellates reached higher abundances in 10-µm treatments, where small ciliate predators and flagellate hunters also (Urotricha spp., Balanion planctonicum) dominated the ciliate assemblage. Overall, our study reports pronounced cascading effects from bacteria to bacterivorous HNF, predatory HNF and ciliates in highly treatment-specific fashions, defined by both prey-food characteristics and feeding modes of predominating protists. Oxford University Press 2020-06-18 /pmc/articles/PMC7538307/ /pubmed/32556274 http://dx.doi.org/10.1093/femsec/fiaa121 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of FEMS. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License(http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Šimek, Karel
Grujčić, Vesna
Mukherjee, Indranil
Kasalický, Vojtěch
Nedoma, Jiří
Posch, Thomas
Mehrshad, Maliheh
Salcher, Michaela M
Cascading effects in freshwater microbial food webs by predatory Cercozoa, Katablepharidacea and ciliates feeding on aplastidic bacterivorous cryptophytes
title Cascading effects in freshwater microbial food webs by predatory Cercozoa, Katablepharidacea and ciliates feeding on aplastidic bacterivorous cryptophytes
title_full Cascading effects in freshwater microbial food webs by predatory Cercozoa, Katablepharidacea and ciliates feeding on aplastidic bacterivorous cryptophytes
title_fullStr Cascading effects in freshwater microbial food webs by predatory Cercozoa, Katablepharidacea and ciliates feeding on aplastidic bacterivorous cryptophytes
title_full_unstemmed Cascading effects in freshwater microbial food webs by predatory Cercozoa, Katablepharidacea and ciliates feeding on aplastidic bacterivorous cryptophytes
title_short Cascading effects in freshwater microbial food webs by predatory Cercozoa, Katablepharidacea and ciliates feeding on aplastidic bacterivorous cryptophytes
title_sort cascading effects in freshwater microbial food webs by predatory cercozoa, katablepharidacea and ciliates feeding on aplastidic bacterivorous cryptophytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538307/
https://www.ncbi.nlm.nih.gov/pubmed/32556274
http://dx.doi.org/10.1093/femsec/fiaa121
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