M1 Macrophage and M1/M2 ratio defined by transcriptomic signatures resemble only part of their conventional clinical characteristics in breast cancer
Tumor associated macrophages (TAMs) play a critical role in biology of various cancers, including breast cancer. In the current study, we defined “M1” macrophage and “M1”/“M2” ratio by transcriptomic signatures using xCell. We investigated the association between high level of “M1” macrophage or “M1...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538579/ https://www.ncbi.nlm.nih.gov/pubmed/33024179 http://dx.doi.org/10.1038/s41598-020-73624-w |
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author | Oshi, Masanori Tokumaru, Yoshihisa Asaoka, Mariko Yan, Li Satyananda, Vikas Matsuyama, Ryusei Matsuhashi, Nobuhisa Futamura, Manabu Ishikawa, Takashi Yoshida, Kazuhiro Endo, Itaru Takabe, Kazuaki |
author_facet | Oshi, Masanori Tokumaru, Yoshihisa Asaoka, Mariko Yan, Li Satyananda, Vikas Matsuyama, Ryusei Matsuhashi, Nobuhisa Futamura, Manabu Ishikawa, Takashi Yoshida, Kazuhiro Endo, Itaru Takabe, Kazuaki |
author_sort | Oshi, Masanori |
collection | PubMed |
description | Tumor associated macrophages (TAMs) play a critical role in biology of various cancers, including breast cancer. In the current study, we defined “M1” macrophage and “M1”/“M2” ratio by transcriptomic signatures using xCell. We investigated the association between high level of “M1” macrophage or “M1”/“M2” ratio and the tumor immune microenvironment by analyzing the transcriptome of publicly available cohorts, TCGA and METABRIC. We found that “M1” high tumors were not associated with prolonged survival compared with “M1” low tumors, or with the response to neoadjuvant chemotherapy. “M1” high tumors were associated with clinically aggressive features and “M1” high tumors enriched the cell proliferation and cell cycle related gene sets in GSEA. At the same time, “M1” high tumors were associated with high immune activity and favorable tumor immune microenvironment, as well as high expression of immune check point molecules. Strikingly, all these results were mirrored in “M1”/“M2” ratio high tumors. In conclusion, transcriptomically defined “M1” or “M1”/“M2” high tumors were associated with aggressive cancer biology and favorable tumor immune microenvironment but not with survival benefit, which resembled only part of their conventional clinical characteristics. |
format | Online Article Text |
id | pubmed-7538579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75385792020-10-07 M1 Macrophage and M1/M2 ratio defined by transcriptomic signatures resemble only part of their conventional clinical characteristics in breast cancer Oshi, Masanori Tokumaru, Yoshihisa Asaoka, Mariko Yan, Li Satyananda, Vikas Matsuyama, Ryusei Matsuhashi, Nobuhisa Futamura, Manabu Ishikawa, Takashi Yoshida, Kazuhiro Endo, Itaru Takabe, Kazuaki Sci Rep Article Tumor associated macrophages (TAMs) play a critical role in biology of various cancers, including breast cancer. In the current study, we defined “M1” macrophage and “M1”/“M2” ratio by transcriptomic signatures using xCell. We investigated the association between high level of “M1” macrophage or “M1”/“M2” ratio and the tumor immune microenvironment by analyzing the transcriptome of publicly available cohorts, TCGA and METABRIC. We found that “M1” high tumors were not associated with prolonged survival compared with “M1” low tumors, or with the response to neoadjuvant chemotherapy. “M1” high tumors were associated with clinically aggressive features and “M1” high tumors enriched the cell proliferation and cell cycle related gene sets in GSEA. At the same time, “M1” high tumors were associated with high immune activity and favorable tumor immune microenvironment, as well as high expression of immune check point molecules. Strikingly, all these results were mirrored in “M1”/“M2” ratio high tumors. In conclusion, transcriptomically defined “M1” or “M1”/“M2” high tumors were associated with aggressive cancer biology and favorable tumor immune microenvironment but not with survival benefit, which resembled only part of their conventional clinical characteristics. Nature Publishing Group UK 2020-10-06 /pmc/articles/PMC7538579/ /pubmed/33024179 http://dx.doi.org/10.1038/s41598-020-73624-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Oshi, Masanori Tokumaru, Yoshihisa Asaoka, Mariko Yan, Li Satyananda, Vikas Matsuyama, Ryusei Matsuhashi, Nobuhisa Futamura, Manabu Ishikawa, Takashi Yoshida, Kazuhiro Endo, Itaru Takabe, Kazuaki M1 Macrophage and M1/M2 ratio defined by transcriptomic signatures resemble only part of their conventional clinical characteristics in breast cancer |
title | M1 Macrophage and M1/M2 ratio defined by transcriptomic signatures resemble only part of their conventional clinical characteristics in breast cancer |
title_full | M1 Macrophage and M1/M2 ratio defined by transcriptomic signatures resemble only part of their conventional clinical characteristics in breast cancer |
title_fullStr | M1 Macrophage and M1/M2 ratio defined by transcriptomic signatures resemble only part of their conventional clinical characteristics in breast cancer |
title_full_unstemmed | M1 Macrophage and M1/M2 ratio defined by transcriptomic signatures resemble only part of their conventional clinical characteristics in breast cancer |
title_short | M1 Macrophage and M1/M2 ratio defined by transcriptomic signatures resemble only part of their conventional clinical characteristics in breast cancer |
title_sort | m1 macrophage and m1/m2 ratio defined by transcriptomic signatures resemble only part of their conventional clinical characteristics in breast cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538579/ https://www.ncbi.nlm.nih.gov/pubmed/33024179 http://dx.doi.org/10.1038/s41598-020-73624-w |
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