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Fruit host-dependent fungal communities in the microbiome of wild Queensland fruit fly larvae

Bactrocera tryoni (Froggatt), the Queensland fruit fly (Qfly), is a highly polyphagous tephritid fly that is widespread in Eastern Australia. Qfly physiology is closely linked with its fungal associates, with particular relationship between Qfly nutrition and yeast or yeast-like fungi. Despite anima...

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Autores principales: Majumder, Rajib, Sutcliffe, Brodie, Taylor, Phillip W., Chapman, Toni A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538879/
https://www.ncbi.nlm.nih.gov/pubmed/33024226
http://dx.doi.org/10.1038/s41598-020-73649-1
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author Majumder, Rajib
Sutcliffe, Brodie
Taylor, Phillip W.
Chapman, Toni A.
author_facet Majumder, Rajib
Sutcliffe, Brodie
Taylor, Phillip W.
Chapman, Toni A.
author_sort Majumder, Rajib
collection PubMed
description Bactrocera tryoni (Froggatt), the Queensland fruit fly (Qfly), is a highly polyphagous tephritid fly that is widespread in Eastern Australia. Qfly physiology is closely linked with its fungal associates, with particular relationship between Qfly nutrition and yeast or yeast-like fungi. Despite animal-associated fungi typically occurring in multi-species communities, Qfly studies have predominately involved the culture and characterisation of single fungal isolates. Further, only two studies have investigated the fungal communities associated with Qfly, and both have used culture-dependant techniques that overlook non-culturable fungi and hence under-represent, and provide a biased interpretation of, the overall fungal community. In order to explore a potentially hidden fungal diversity and complexity within the Qfly mycobiome, we used culture-independent, high-throughput Illumina sequencing techniques to comprehensively, and holistically characterized the fungal community of Qfly larvae and overcome the culture bias. We collected larvae from a range of fruit hosts along the east coast of Australia, and all had a mycobiome dominated by ascomycetes. The most abundant fungal taxa belonged to the genera Pichia (43%), Candida (20%), Hanseniaspora (10%), Zygosaccharomyces (11%) and Penicillium (7%). We also characterized the fungal communities of fruit hosts, and found a strong degree of overlap between larvae and fruit host communities, suggesting that these communities are intimately inter-connected. Our data suggests that larval fungal communities are acquired from surrounding fruit flesh. It is likely that the physiological benefits of Qfly exposure to fungal communities is primarily due to consumption of these fungi, not through syntrophy/symbiosis between fungi and insect ‘host’.
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spelling pubmed-75388792020-10-07 Fruit host-dependent fungal communities in the microbiome of wild Queensland fruit fly larvae Majumder, Rajib Sutcliffe, Brodie Taylor, Phillip W. Chapman, Toni A. Sci Rep Article Bactrocera tryoni (Froggatt), the Queensland fruit fly (Qfly), is a highly polyphagous tephritid fly that is widespread in Eastern Australia. Qfly physiology is closely linked with its fungal associates, with particular relationship between Qfly nutrition and yeast or yeast-like fungi. Despite animal-associated fungi typically occurring in multi-species communities, Qfly studies have predominately involved the culture and characterisation of single fungal isolates. Further, only two studies have investigated the fungal communities associated with Qfly, and both have used culture-dependant techniques that overlook non-culturable fungi and hence under-represent, and provide a biased interpretation of, the overall fungal community. In order to explore a potentially hidden fungal diversity and complexity within the Qfly mycobiome, we used culture-independent, high-throughput Illumina sequencing techniques to comprehensively, and holistically characterized the fungal community of Qfly larvae and overcome the culture bias. We collected larvae from a range of fruit hosts along the east coast of Australia, and all had a mycobiome dominated by ascomycetes. The most abundant fungal taxa belonged to the genera Pichia (43%), Candida (20%), Hanseniaspora (10%), Zygosaccharomyces (11%) and Penicillium (7%). We also characterized the fungal communities of fruit hosts, and found a strong degree of overlap between larvae and fruit host communities, suggesting that these communities are intimately inter-connected. Our data suggests that larval fungal communities are acquired from surrounding fruit flesh. It is likely that the physiological benefits of Qfly exposure to fungal communities is primarily due to consumption of these fungi, not through syntrophy/symbiosis between fungi and insect ‘host’. Nature Publishing Group UK 2020-10-06 /pmc/articles/PMC7538879/ /pubmed/33024226 http://dx.doi.org/10.1038/s41598-020-73649-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Majumder, Rajib
Sutcliffe, Brodie
Taylor, Phillip W.
Chapman, Toni A.
Fruit host-dependent fungal communities in the microbiome of wild Queensland fruit fly larvae
title Fruit host-dependent fungal communities in the microbiome of wild Queensland fruit fly larvae
title_full Fruit host-dependent fungal communities in the microbiome of wild Queensland fruit fly larvae
title_fullStr Fruit host-dependent fungal communities in the microbiome of wild Queensland fruit fly larvae
title_full_unstemmed Fruit host-dependent fungal communities in the microbiome of wild Queensland fruit fly larvae
title_short Fruit host-dependent fungal communities in the microbiome of wild Queensland fruit fly larvae
title_sort fruit host-dependent fungal communities in the microbiome of wild queensland fruit fly larvae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538879/
https://www.ncbi.nlm.nih.gov/pubmed/33024226
http://dx.doi.org/10.1038/s41598-020-73649-1
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