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Do human embryos have the ability of self-correction?

Human embryogenesis frequently coinciding with cell division mistakes contributing to pervasive embryonic aneuploidy/mosaicism. While embryo self-correction was elegantly demonstrated in mouse models, human studies are lacking. Here we are witness to human embryos ability to eliminate/expel abnormal...

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Autores principales: Orvieto, Raoul, Shimon, Chen, Rienstein, Shlomit, Jonish-Grossman, Anat, Shani, Hagit, Aizer, Adva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7539487/
https://www.ncbi.nlm.nih.gov/pubmed/33023576
http://dx.doi.org/10.1186/s12958-020-00650-8
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author Orvieto, Raoul
Shimon, Chen
Rienstein, Shlomit
Jonish-Grossman, Anat
Shani, Hagit
Aizer, Adva
author_facet Orvieto, Raoul
Shimon, Chen
Rienstein, Shlomit
Jonish-Grossman, Anat
Shani, Hagit
Aizer, Adva
author_sort Orvieto, Raoul
collection PubMed
description Human embryogenesis frequently coinciding with cell division mistakes contributing to pervasive embryonic aneuploidy/mosaicism. While embryo self-correction was elegantly demonstrated in mouse models, human studies are lacking. Here we are witness to human embryos ability to eliminate/expel abnormal blastomeres as cell debris/fragments. Each blastocyst and its corresponding debris were separated and underwent whole genome amplification. Seven of the 11 pairs of blastocysts and their corresponding cell debris/fragments revealed discordant results. Of the 9 euploid blastocysts, four showed euploid debris, while in the others, the debris were aneuploid. In the remaining pairs, the debris showed additional aneuploidy to those presented by their corresponding blastocyst. The observed ability of human embryos to self-correction doubts many invasive and non-invasive preimplantation testing for aneuploidy at the blastocyst stage, rendering high rate of false positive (discarding “good” embryos) by identifying the cell-free DNA originated from the expelled cell debris, as aneuploidy/mosaic blastocyst.
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spelling pubmed-75394872020-10-08 Do human embryos have the ability of self-correction? Orvieto, Raoul Shimon, Chen Rienstein, Shlomit Jonish-Grossman, Anat Shani, Hagit Aizer, Adva Reprod Biol Endocrinol Research Human embryogenesis frequently coinciding with cell division mistakes contributing to pervasive embryonic aneuploidy/mosaicism. While embryo self-correction was elegantly demonstrated in mouse models, human studies are lacking. Here we are witness to human embryos ability to eliminate/expel abnormal blastomeres as cell debris/fragments. Each blastocyst and its corresponding debris were separated and underwent whole genome amplification. Seven of the 11 pairs of blastocysts and their corresponding cell debris/fragments revealed discordant results. Of the 9 euploid blastocysts, four showed euploid debris, while in the others, the debris were aneuploid. In the remaining pairs, the debris showed additional aneuploidy to those presented by their corresponding blastocyst. The observed ability of human embryos to self-correction doubts many invasive and non-invasive preimplantation testing for aneuploidy at the blastocyst stage, rendering high rate of false positive (discarding “good” embryos) by identifying the cell-free DNA originated from the expelled cell debris, as aneuploidy/mosaic blastocyst. BioMed Central 2020-10-06 /pmc/articles/PMC7539487/ /pubmed/33023576 http://dx.doi.org/10.1186/s12958-020-00650-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Orvieto, Raoul
Shimon, Chen
Rienstein, Shlomit
Jonish-Grossman, Anat
Shani, Hagit
Aizer, Adva
Do human embryos have the ability of self-correction?
title Do human embryos have the ability of self-correction?
title_full Do human embryos have the ability of self-correction?
title_fullStr Do human embryos have the ability of self-correction?
title_full_unstemmed Do human embryos have the ability of self-correction?
title_short Do human embryos have the ability of self-correction?
title_sort do human embryos have the ability of self-correction?
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7539487/
https://www.ncbi.nlm.nih.gov/pubmed/33023576
http://dx.doi.org/10.1186/s12958-020-00650-8
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