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Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector
Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was classified as a pandemic by the World Health Organization and has caused over 550,000 deaths worldwide as of July 2020. Accurate and scalable point-of-care devices would increase screening, diag...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7539830/ https://www.ncbi.nlm.nih.gov/pubmed/33075724 http://dx.doi.org/10.1016/j.bios.2020.112709 |
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author | Rashed, Mohamed Z. Kopechek, Jonathan A. Priddy, Mariah C. Hamorsky, Krystal T. Palmer, Kenneth E. Mittal, Nikhil Valdez, Joseph Flynn, Joseph Williams, Stuart J. |
author_facet | Rashed, Mohamed Z. Kopechek, Jonathan A. Priddy, Mariah C. Hamorsky, Krystal T. Palmer, Kenneth E. Mittal, Nikhil Valdez, Joseph Flynn, Joseph Williams, Stuart J. |
author_sort | Rashed, Mohamed Z. |
collection | PubMed |
description | Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was classified as a pandemic by the World Health Organization and has caused over 550,000 deaths worldwide as of July 2020. Accurate and scalable point-of-care devices would increase screening, diagnosis, and monitoring of COVID-19 patients. Here, we demonstrate rapid label-free electrochemical detection of SARS-CoV-2 antibodies using a commercially available impedance sensing platform. A 16-well plate containing sensing electrodes was pre-coated with receptor binding domain (RBD) of SARS-CoV-2 spike protein, and subsequently tested with samples of anti-SARS-CoV-2 monoclonal antibody CR3022 (0.1 [Formula: see text] g/ml, 1.0 [Formula: see text] g/ml, 10 [Formula: see text] g/ml). Subsequent blinded testing was performed on six serum specimens taken from COVID-19 and non-COVID-19 patients (1:100 dilution factor). The platform was able to differentiate spikes in impedance measurements from a negative control (1% milk solution) for all CR3022 samples. Further, successful differentiation and detection of all positive clinical samples from negative control was achieved. Measured impedance values were consistent when compared to standard ELISA test results showing a strong correlation between them (R [Formula: see text]). Detection occurs in less than five minutes and the well-based platform provides a simplified and familiar testing interface that can be readily adaptable for use in clinical settings. |
format | Online Article Text |
id | pubmed-7539830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75398302020-10-08 Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector Rashed, Mohamed Z. Kopechek, Jonathan A. Priddy, Mariah C. Hamorsky, Krystal T. Palmer, Kenneth E. Mittal, Nikhil Valdez, Joseph Flynn, Joseph Williams, Stuart J. Biosens Bioelectron Article Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was classified as a pandemic by the World Health Organization and has caused over 550,000 deaths worldwide as of July 2020. Accurate and scalable point-of-care devices would increase screening, diagnosis, and monitoring of COVID-19 patients. Here, we demonstrate rapid label-free electrochemical detection of SARS-CoV-2 antibodies using a commercially available impedance sensing platform. A 16-well plate containing sensing electrodes was pre-coated with receptor binding domain (RBD) of SARS-CoV-2 spike protein, and subsequently tested with samples of anti-SARS-CoV-2 monoclonal antibody CR3022 (0.1 [Formula: see text] g/ml, 1.0 [Formula: see text] g/ml, 10 [Formula: see text] g/ml). Subsequent blinded testing was performed on six serum specimens taken from COVID-19 and non-COVID-19 patients (1:100 dilution factor). The platform was able to differentiate spikes in impedance measurements from a negative control (1% milk solution) for all CR3022 samples. Further, successful differentiation and detection of all positive clinical samples from negative control was achieved. Measured impedance values were consistent when compared to standard ELISA test results showing a strong correlation between them (R [Formula: see text]). Detection occurs in less than five minutes and the well-based platform provides a simplified and familiar testing interface that can be readily adaptable for use in clinical settings. Elsevier B.V. 2021-01-01 2020-10-07 /pmc/articles/PMC7539830/ /pubmed/33075724 http://dx.doi.org/10.1016/j.bios.2020.112709 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Rashed, Mohamed Z. Kopechek, Jonathan A. Priddy, Mariah C. Hamorsky, Krystal T. Palmer, Kenneth E. Mittal, Nikhil Valdez, Joseph Flynn, Joseph Williams, Stuart J. Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector |
title | Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector |
title_full | Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector |
title_fullStr | Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector |
title_full_unstemmed | Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector |
title_short | Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector |
title_sort | rapid detection of sars-cov-2 antibodies using electrochemical impedance-based detector |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7539830/ https://www.ncbi.nlm.nih.gov/pubmed/33075724 http://dx.doi.org/10.1016/j.bios.2020.112709 |
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