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Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR
With the increasing immunological studies on camels due to the advantage of their single-chain antibodies for humanizations, it is demanding to develop an easy-to-handle evaluation method of their humoral immune response before proceeding with immunization of foreign antigens that may be toxic to ca...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Editorial Department of Journal of Biomedical Research
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540241/ https://www.ncbi.nlm.nih.gov/pubmed/32611846 http://dx.doi.org/10.7555/JBR.34.20190035 |
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author | Yu, Xinyu Wu, Yuan Zhang, Jiarong Jirimutu, Zulipikaer, Azhati Chen, Jin |
author_facet | Yu, Xinyu Wu, Yuan Zhang, Jiarong Jirimutu, Zulipikaer, Azhati Chen, Jin |
author_sort | Yu, Xinyu |
collection | PubMed |
description | With the increasing immunological studies on camels due to the advantage of their single-chain antibodies for humanizations, it is demanding to develop an easy-to-handle evaluation method of their humoral immune response before proceeding with immunization of foreign antigens that may be toxic to camels. In this study, we quantitatively determined the expression levels of T-helper 2 (Th2) cytokines in peripheral blood lymphocytes obtained from Bactrian camels by real-time PCR. The recorded kinetic profiles resulting from the immunization of ovalbumin (OVA) indicated that after immunization, Th2 cytokines including interleukin (IL) families such as IL-4, IL-10, and IL-13 in the camels were up-regulated by a factor of 1.78, 3.15, and 1.22, respectively, which was validated by traditional enzyme-linked immunosorbent assay (ELISA) methods. Unlike ELISA which requires specific enzyme-labeled antibodies, this established method based on the minimal amount of blood samples holds an advantage in the preliminary evaluation of camel humoral immune response with desirable precision, which is meaningful for biomedical explorations of camel-derived antibodies. |
format | Online Article Text |
id | pubmed-7540241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Editorial Department of Journal of Biomedical Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-75402412020-10-30 Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR Yu, Xinyu Wu, Yuan Zhang, Jiarong Jirimutu, Zulipikaer, Azhati Chen, Jin J Biomed Res Brief Report With the increasing immunological studies on camels due to the advantage of their single-chain antibodies for humanizations, it is demanding to develop an easy-to-handle evaluation method of their humoral immune response before proceeding with immunization of foreign antigens that may be toxic to camels. In this study, we quantitatively determined the expression levels of T-helper 2 (Th2) cytokines in peripheral blood lymphocytes obtained from Bactrian camels by real-time PCR. The recorded kinetic profiles resulting from the immunization of ovalbumin (OVA) indicated that after immunization, Th2 cytokines including interleukin (IL) families such as IL-4, IL-10, and IL-13 in the camels were up-regulated by a factor of 1.78, 3.15, and 1.22, respectively, which was validated by traditional enzyme-linked immunosorbent assay (ELISA) methods. Unlike ELISA which requires specific enzyme-labeled antibodies, this established method based on the minimal amount of blood samples holds an advantage in the preliminary evaluation of camel humoral immune response with desirable precision, which is meaningful for biomedical explorations of camel-derived antibodies. Editorial Department of Journal of Biomedical Research 2020-09 /pmc/articles/PMC7540241/ /pubmed/32611846 http://dx.doi.org/10.7555/JBR.34.20190035 Text en Copyright and License information: Journal of Biomedical Research, CAS Springer-Verlag Berlin Heidelberg 2020 http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Brief Report Yu, Xinyu Wu, Yuan Zhang, Jiarong Jirimutu, Zulipikaer, Azhati Chen, Jin Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR |
title | Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR |
title_full | Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR |
title_fullStr | Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR |
title_full_unstemmed | Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR |
title_short | Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR |
title_sort | pre-evaluation of humoral immune response of bactrian camels by the quantification of th2 cytokines using real-time pcr |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540241/ https://www.ncbi.nlm.nih.gov/pubmed/32611846 http://dx.doi.org/10.7555/JBR.34.20190035 |
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