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In Vivo Flow Cytometric Evaluation of Circulating Metastatic Pancreatic Tumor Cells after High‐Intensity Focused Ultrasound Therapy

We examined our hypothesis that high‐intensity focused ultrasound (HIFU) treatment of pancreatic ductal adenocarcinoma (PDAC) in nude mice models may lead to an increased occurrence of hematogenous metastasis. The human PDAC cell line BxPC‐3 transfected with mCherry was implanted into nude mice to e...

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Autores principales: Yu, Qian, Yao, Yijing, Zhu, Xi, Gao, Yihui, Chen, Yini, Wang, Rui, Xu, Pingping, Wei, Xunbin, Jiang, Lixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540359/
https://www.ncbi.nlm.nih.gov/pubmed/32307867
http://dx.doi.org/10.1002/cyto.a.24014
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author Yu, Qian
Yao, Yijing
Zhu, Xi
Gao, Yihui
Chen, Yini
Wang, Rui
Xu, Pingping
Wei, Xunbin
Jiang, Lixin
author_facet Yu, Qian
Yao, Yijing
Zhu, Xi
Gao, Yihui
Chen, Yini
Wang, Rui
Xu, Pingping
Wei, Xunbin
Jiang, Lixin
author_sort Yu, Qian
collection PubMed
description We examined our hypothesis that high‐intensity focused ultrasound (HIFU) treatment of pancreatic ductal adenocarcinoma (PDAC) in nude mice models may lead to an increased occurrence of hematogenous metastasis. The human PDAC cell line BxPC‐3 transfected with mCherry was implanted into nude mice to establish orthotopic and subcutaneous xenograft (OX and SX) tumor models. Mice were exposed to HIFU when tumor sizes reached approximately 200–300 mm(3). The OX and SX tumor models were monitored continuously for tumor growth characteristics and hematogenous metastasis using in vivo flow cytometric (IVFC) detection of circulating tumor cells (CTCs) from the pancreas. We chose an appropriate mouse model to further examine whether or not HIFU increases the potential risk of hematogenous metastasis, using IVFC detection. Our results showed that the CTC number was greater in the OX model than in the SX model. The CTC number in the OX model increased gradually over time, whereas the CTC number in the SX model remained low. Therefore, the OX model was better for studying tumor metastasis by IVFC detection. We found significantly decreased CTC numbers and tumor volume after HIFU ablation. Our results showed the applicability of the PDAC OX tumor model for studying the occurrence of tumor metastasis due to the generation of CTCs. HIFU ablation substantially restricted PDAC hematogenous metastasis and provided effective tumor control locally. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals Inc., on behalf of International Society for Advancement of Cytometry.
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spelling pubmed-75403592020-10-09 In Vivo Flow Cytometric Evaluation of Circulating Metastatic Pancreatic Tumor Cells after High‐Intensity Focused Ultrasound Therapy Yu, Qian Yao, Yijing Zhu, Xi Gao, Yihui Chen, Yini Wang, Rui Xu, Pingping Wei, Xunbin Jiang, Lixin Cytometry A Original Articles We examined our hypothesis that high‐intensity focused ultrasound (HIFU) treatment of pancreatic ductal adenocarcinoma (PDAC) in nude mice models may lead to an increased occurrence of hematogenous metastasis. The human PDAC cell line BxPC‐3 transfected with mCherry was implanted into nude mice to establish orthotopic and subcutaneous xenograft (OX and SX) tumor models. Mice were exposed to HIFU when tumor sizes reached approximately 200–300 mm(3). The OX and SX tumor models were monitored continuously for tumor growth characteristics and hematogenous metastasis using in vivo flow cytometric (IVFC) detection of circulating tumor cells (CTCs) from the pancreas. We chose an appropriate mouse model to further examine whether or not HIFU increases the potential risk of hematogenous metastasis, using IVFC detection. Our results showed that the CTC number was greater in the OX model than in the SX model. The CTC number in the OX model increased gradually over time, whereas the CTC number in the SX model remained low. Therefore, the OX model was better for studying tumor metastasis by IVFC detection. We found significantly decreased CTC numbers and tumor volume after HIFU ablation. Our results showed the applicability of the PDAC OX tumor model for studying the occurrence of tumor metastasis due to the generation of CTCs. HIFU ablation substantially restricted PDAC hematogenous metastasis and provided effective tumor control locally. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals Inc., on behalf of International Society for Advancement of Cytometry. John Wiley & Sons, Inc. 2020-04-19 2020-09 /pmc/articles/PMC7540359/ /pubmed/32307867 http://dx.doi.org/10.1002/cyto.a.24014 Text en © 2020 The Authors. Cytometry Part A published by Wiley Periodicals Inc., on behalf of International Society for Advancement of Cytometry. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Yu, Qian
Yao, Yijing
Zhu, Xi
Gao, Yihui
Chen, Yini
Wang, Rui
Xu, Pingping
Wei, Xunbin
Jiang, Lixin
In Vivo Flow Cytometric Evaluation of Circulating Metastatic Pancreatic Tumor Cells after High‐Intensity Focused Ultrasound Therapy
title In Vivo Flow Cytometric Evaluation of Circulating Metastatic Pancreatic Tumor Cells after High‐Intensity Focused Ultrasound Therapy
title_full In Vivo Flow Cytometric Evaluation of Circulating Metastatic Pancreatic Tumor Cells after High‐Intensity Focused Ultrasound Therapy
title_fullStr In Vivo Flow Cytometric Evaluation of Circulating Metastatic Pancreatic Tumor Cells after High‐Intensity Focused Ultrasound Therapy
title_full_unstemmed In Vivo Flow Cytometric Evaluation of Circulating Metastatic Pancreatic Tumor Cells after High‐Intensity Focused Ultrasound Therapy
title_short In Vivo Flow Cytometric Evaluation of Circulating Metastatic Pancreatic Tumor Cells after High‐Intensity Focused Ultrasound Therapy
title_sort in vivo flow cytometric evaluation of circulating metastatic pancreatic tumor cells after high‐intensity focused ultrasound therapy
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540359/
https://www.ncbi.nlm.nih.gov/pubmed/32307867
http://dx.doi.org/10.1002/cyto.a.24014
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