Reprogramming Urine‐Derived Cells using Commercially Available Self‐Replicative RNA and a Single Electroporation

We describe a protocol for efficient generation of human‐induced pluripotent stem cells (hiPSCs) from urine‐derived cells (UDCs) obtained from adult donors using self‐replicative RNA containing the reprogramming factors OCT3/4, SOX2, KLF4, GLIS1, and c‐MYC (ReproRNA‐OKSGM). After electroporation, tr...

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Detalles Bibliográficos
Autores principales: Bouma, Marga J., Arendzen, Christiaan H., Mummery, Christine L., Mikkers, Harald, Freund, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540473/
https://www.ncbi.nlm.nih.gov/pubmed/32956580
http://dx.doi.org/10.1002/cpsc.124

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540473/
https://www.ncbi.nlm.nih.gov/pubmed/32956580
http://dx.doi.org/10.1002/cpsc.124

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