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Comparison of the cytotoxic effects of different fractions of Artemisia ciniformis and Artemisia biennis on B16/F10, PC3 and MCF7 Cells

BACKGROUND AND PURPOSE: Artemisia is one of the well-known herbal medicinal plants for antimicrobial, insecticidal, antioxidant, and antimalarial activities. The antiproliferative effects of dichloromethane extracts of Artemisia biennis (A. biennis) and A. ciniformis and the petroleum ether extract...

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Autores principales: Ramazani, Elham, Tayarani-Najaran, Zahra, Shokoohinia, Yalda, Mojarrab, Mahdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540818/
https://www.ncbi.nlm.nih.gov/pubmed/33088327
http://dx.doi.org/10.4103/1735-5362.288434
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author Ramazani, Elham
Tayarani-Najaran, Zahra
Shokoohinia, Yalda
Mojarrab, Mahdi
author_facet Ramazani, Elham
Tayarani-Najaran, Zahra
Shokoohinia, Yalda
Mojarrab, Mahdi
author_sort Ramazani, Elham
collection PubMed
description BACKGROUND AND PURPOSE: Artemisia is one of the well-known herbal medicinal plants for antimicrobial, insecticidal, antioxidant, and antimalarial activities. The antiproliferative effects of dichloromethane extracts of Artemisia biennis (A. biennis) and A. ciniformis and the petroleum ether extract of A. ciniformis have been demonstrated previously on human cancerous cell lines. In the current study, further fractionation was carried out on the aforementioned extracts and their cytotoxic effects were evaluated on three human cancer cell lines; B16/F10, PC3, and MCF7. F1 to F16, F1’ to F11’, and F1” to F10” were resulted from the fractionation of dichloromethane extracts of A. biennis, A. ciniformis, and petroleum ether extract of A. ciniformis, respectively. EXPERIMENTAL APPROACH: The cytotoxic effects of 16 (F1-F16), 11 (F1’-F11’) and 10 (F1”-F10”) fractions, on B16/F10, PC3, and MCF7 cell lines were assessed using resazurin to measure viability and propidium iodide staining (sub G1) and flow cytometry to detect apoptosis. FINDINGS / RESULTS: The results showed that, some fractions at 100 μg/mL decreased cell viability. F2” in B16/F10 cells, F2, F4-F6, F10’, F11’, and F2” in PC3 cells, and F10’, F11’, and F2” in MCF7 significantly decreased cell viability in a concentration-dependent manner (12.5-50 μg/mL). Among different fractions, F2” demonstrated the most potent cytotoxic effects on cancer cell lines (P < 0.001). All of the mentioned fractions (except F11’ on PC3 cells) increased the number of apoptotic cells and showed the cytotoxic effects on cancer cells compared with the control group. CONCLUSION AND IMPLICATIONS: A. biennis and A. ciniformis are suggested as the potential sources of cytotoxic phytochemicals. The probable presence of terpenoids, steroids, and alkaloids in the selected fractions is proposed based on the preliminary phytochemical study.
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spelling pubmed-75408182020-10-20 Comparison of the cytotoxic effects of different fractions of Artemisia ciniformis and Artemisia biennis on B16/F10, PC3 and MCF7 Cells Ramazani, Elham Tayarani-Najaran, Zahra Shokoohinia, Yalda Mojarrab, Mahdi Res Pharm Sci Original Article BACKGROUND AND PURPOSE: Artemisia is one of the well-known herbal medicinal plants for antimicrobial, insecticidal, antioxidant, and antimalarial activities. The antiproliferative effects of dichloromethane extracts of Artemisia biennis (A. biennis) and A. ciniformis and the petroleum ether extract of A. ciniformis have been demonstrated previously on human cancerous cell lines. In the current study, further fractionation was carried out on the aforementioned extracts and their cytotoxic effects were evaluated on three human cancer cell lines; B16/F10, PC3, and MCF7. F1 to F16, F1’ to F11’, and F1” to F10” were resulted from the fractionation of dichloromethane extracts of A. biennis, A. ciniformis, and petroleum ether extract of A. ciniformis, respectively. EXPERIMENTAL APPROACH: The cytotoxic effects of 16 (F1-F16), 11 (F1’-F11’) and 10 (F1”-F10”) fractions, on B16/F10, PC3, and MCF7 cell lines were assessed using resazurin to measure viability and propidium iodide staining (sub G1) and flow cytometry to detect apoptosis. FINDINGS / RESULTS: The results showed that, some fractions at 100 μg/mL decreased cell viability. F2” in B16/F10 cells, F2, F4-F6, F10’, F11’, and F2” in PC3 cells, and F10’, F11’, and F2” in MCF7 significantly decreased cell viability in a concentration-dependent manner (12.5-50 μg/mL). Among different fractions, F2” demonstrated the most potent cytotoxic effects on cancer cell lines (P < 0.001). All of the mentioned fractions (except F11’ on PC3 cells) increased the number of apoptotic cells and showed the cytotoxic effects on cancer cells compared with the control group. CONCLUSION AND IMPLICATIONS: A. biennis and A. ciniformis are suggested as the potential sources of cytotoxic phytochemicals. The probable presence of terpenoids, steroids, and alkaloids in the selected fractions is proposed based on the preliminary phytochemical study. Wolters Kluwer - Medknow 2020-07-03 /pmc/articles/PMC7540818/ /pubmed/33088327 http://dx.doi.org/10.4103/1735-5362.288434 Text en Copyright: © 2020 Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Ramazani, Elham
Tayarani-Najaran, Zahra
Shokoohinia, Yalda
Mojarrab, Mahdi
Comparison of the cytotoxic effects of different fractions of Artemisia ciniformis and Artemisia biennis on B16/F10, PC3 and MCF7 Cells
title Comparison of the cytotoxic effects of different fractions of Artemisia ciniformis and Artemisia biennis on B16/F10, PC3 and MCF7 Cells
title_full Comparison of the cytotoxic effects of different fractions of Artemisia ciniformis and Artemisia biennis on B16/F10, PC3 and MCF7 Cells
title_fullStr Comparison of the cytotoxic effects of different fractions of Artemisia ciniformis and Artemisia biennis on B16/F10, PC3 and MCF7 Cells
title_full_unstemmed Comparison of the cytotoxic effects of different fractions of Artemisia ciniformis and Artemisia biennis on B16/F10, PC3 and MCF7 Cells
title_short Comparison of the cytotoxic effects of different fractions of Artemisia ciniformis and Artemisia biennis on B16/F10, PC3 and MCF7 Cells
title_sort comparison of the cytotoxic effects of different fractions of artemisia ciniformis and artemisia biennis on b16/f10, pc3 and mcf7 cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540818/
https://www.ncbi.nlm.nih.gov/pubmed/33088327
http://dx.doi.org/10.4103/1735-5362.288434
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