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High-throughput, image-based phenotyping reveals nutrient-dependent growth facilitation in a grass-legume mixture
This study used high throughput, image-based phenotyping (HTP) to distinguish growth patterns, detect facilitation and interpret variations to nutrient uptake in a model mixed-pasture system in response to factorial low and high nitrogen (N) and phosphorus (P) application. HTP has not previously bee...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540849/ https://www.ncbi.nlm.nih.gov/pubmed/33027289 http://dx.doi.org/10.1371/journal.pone.0239673 |
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author | Ball, Kirsten Rae Power, Sally Anne Brien, Chris Woodin, Sarah Jewell, Nathaniel Berger, Bettina Pendall, Elise |
author_facet | Ball, Kirsten Rae Power, Sally Anne Brien, Chris Woodin, Sarah Jewell, Nathaniel Berger, Bettina Pendall, Elise |
author_sort | Ball, Kirsten Rae |
collection | PubMed |
description | This study used high throughput, image-based phenotyping (HTP) to distinguish growth patterns, detect facilitation and interpret variations to nutrient uptake in a model mixed-pasture system in response to factorial low and high nitrogen (N) and phosphorus (P) application. HTP has not previously been used to examine pasture species in mixture. We used red-green-blue (RGB) imaging to obtain smoothed projected shoot area (sPSA) to predict absolute growth (AG) up to 70 days after planting (sPSA, DAP 70), to identify variation in relative growth rates (RGR, DAP 35–70) and detect overyielding (an increase in yield in mixture compared with monoculture, indicating facilitation) in a grass-legume model pasture. Finally, using principal components analysis we interpreted between species changes to HTP-derived temporal growth dynamics and nutrient uptake in mixtures and monocultures. Overyielding was detected in all treatments and was driven by both grass and legume. Our data supported expectations of more rapid grass growth and augmented nutrient uptake in the presence of a legume. Legumes grew more slowly in mixture and where growth became more reliant on soil P. Relative growth rate in grass was strongly associated with shoot N concentration, whereas legume RGR was not strongly associated with shoot nutrients. High throughput, image-based phenotyping was a useful tool to quantify growth trait variation between contrasting species and to this end is highly useful in understanding nutrient-yield relationships in mixed pasture cultivations. |
format | Online Article Text |
id | pubmed-7540849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-75408492020-10-19 High-throughput, image-based phenotyping reveals nutrient-dependent growth facilitation in a grass-legume mixture Ball, Kirsten Rae Power, Sally Anne Brien, Chris Woodin, Sarah Jewell, Nathaniel Berger, Bettina Pendall, Elise PLoS One Research Article This study used high throughput, image-based phenotyping (HTP) to distinguish growth patterns, detect facilitation and interpret variations to nutrient uptake in a model mixed-pasture system in response to factorial low and high nitrogen (N) and phosphorus (P) application. HTP has not previously been used to examine pasture species in mixture. We used red-green-blue (RGB) imaging to obtain smoothed projected shoot area (sPSA) to predict absolute growth (AG) up to 70 days after planting (sPSA, DAP 70), to identify variation in relative growth rates (RGR, DAP 35–70) and detect overyielding (an increase in yield in mixture compared with monoculture, indicating facilitation) in a grass-legume model pasture. Finally, using principal components analysis we interpreted between species changes to HTP-derived temporal growth dynamics and nutrient uptake in mixtures and monocultures. Overyielding was detected in all treatments and was driven by both grass and legume. Our data supported expectations of more rapid grass growth and augmented nutrient uptake in the presence of a legume. Legumes grew more slowly in mixture and where growth became more reliant on soil P. Relative growth rate in grass was strongly associated with shoot N concentration, whereas legume RGR was not strongly associated with shoot nutrients. High throughput, image-based phenotyping was a useful tool to quantify growth trait variation between contrasting species and to this end is highly useful in understanding nutrient-yield relationships in mixed pasture cultivations. Public Library of Science 2020-10-07 /pmc/articles/PMC7540849/ /pubmed/33027289 http://dx.doi.org/10.1371/journal.pone.0239673 Text en © 2020 Ball et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ball, Kirsten Rae Power, Sally Anne Brien, Chris Woodin, Sarah Jewell, Nathaniel Berger, Bettina Pendall, Elise High-throughput, image-based phenotyping reveals nutrient-dependent growth facilitation in a grass-legume mixture |
title | High-throughput, image-based phenotyping reveals nutrient-dependent growth facilitation in a grass-legume mixture |
title_full | High-throughput, image-based phenotyping reveals nutrient-dependent growth facilitation in a grass-legume mixture |
title_fullStr | High-throughput, image-based phenotyping reveals nutrient-dependent growth facilitation in a grass-legume mixture |
title_full_unstemmed | High-throughput, image-based phenotyping reveals nutrient-dependent growth facilitation in a grass-legume mixture |
title_short | High-throughput, image-based phenotyping reveals nutrient-dependent growth facilitation in a grass-legume mixture |
title_sort | high-throughput, image-based phenotyping reveals nutrient-dependent growth facilitation in a grass-legume mixture |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540849/ https://www.ncbi.nlm.nih.gov/pubmed/33027289 http://dx.doi.org/10.1371/journal.pone.0239673 |
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