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Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo

Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum, is one of the world’s most destructive diseases of common bean. The use of resistant cultivars is the most cost-effective strategy to manage this disease; however, durable resistance is difficult to achieve due to the vast vir...

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Autores principales: Gilio, Thiago Alexandre Santana, Hurtado-Gonzales, Oscar P., Gonçalves-Vidigal, Maria Celeste, Valentini, Giseli, Ferreira Elias, Julio Cesar, Song, Qijian, Pastor-Corrales, Marcial A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540868/
https://www.ncbi.nlm.nih.gov/pubmed/33027258
http://dx.doi.org/10.1371/journal.pone.0239763
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author Gilio, Thiago Alexandre Santana
Hurtado-Gonzales, Oscar P.
Gonçalves-Vidigal, Maria Celeste
Valentini, Giseli
Ferreira Elias, Julio Cesar
Song, Qijian
Pastor-Corrales, Marcial A.
author_facet Gilio, Thiago Alexandre Santana
Hurtado-Gonzales, Oscar P.
Gonçalves-Vidigal, Maria Celeste
Valentini, Giseli
Ferreira Elias, Julio Cesar
Song, Qijian
Pastor-Corrales, Marcial A.
author_sort Gilio, Thiago Alexandre Santana
collection PubMed
description Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum, is one of the world’s most destructive diseases of common bean. The use of resistant cultivars is the most cost-effective strategy to manage this disease; however, durable resistance is difficult to achieve due to the vast virulence diversity of the anthracnose pathogen. Finding new genes with broad-spectrum resistance increases the prospect of designing an effective anthracnose-management strategy. Genetic analysis confirmed the presence of a single, dominant anthracnose-resistance locus in AC, which we provisionally named Co-AC. Bulk segregant analysis and genetic mapping of two F(2) populations from the crosses AC × PI207262 and AC × G 2333 were used to determine the position of the Co-AC locus in a 631 Kbp genomic region flanked by the SNP markers SS56 and SS92 on the lower arm of chromosome Pv01. By genotyping 77 F(3) plants from the AC × PI207262 cross using nine additional markers, we fine-mapped the Co-AC locus to a significantly smaller genomic region (9.4 Kbp) flanked by the SNP markers SS102 and SS165. This 9.4 Kbp region harbors three predicted genes based on the common bean reference genome, notably including the gene model Phvul.001G244300, which encodes Clathrin heavy chain 1, a protein that supports specific stomatal regulation functions and might play a role in plant defense signaling. Because the Co-AC resistance locus is linked in cis, it can be selected with great efficiency using molecular markers. These results will be very useful for breeding programs aimed at developing bean cultivars with anthracnose resistance using marker-assisted selection. This study revealed the broad-spectrum resistance of AC to C. lindemuthianum and the existence of the Co-AC anthracnose-resistance locus. Fine mapping positioned this locus in a small genomic region on the lower end of chromosome Pv01 that contained three candidate genes for the Co-AC locus.
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spelling pubmed-75408682020-10-19 Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo Gilio, Thiago Alexandre Santana Hurtado-Gonzales, Oscar P. Gonçalves-Vidigal, Maria Celeste Valentini, Giseli Ferreira Elias, Julio Cesar Song, Qijian Pastor-Corrales, Marcial A. PLoS One Research Article Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum, is one of the world’s most destructive diseases of common bean. The use of resistant cultivars is the most cost-effective strategy to manage this disease; however, durable resistance is difficult to achieve due to the vast virulence diversity of the anthracnose pathogen. Finding new genes with broad-spectrum resistance increases the prospect of designing an effective anthracnose-management strategy. Genetic analysis confirmed the presence of a single, dominant anthracnose-resistance locus in AC, which we provisionally named Co-AC. Bulk segregant analysis and genetic mapping of two F(2) populations from the crosses AC × PI207262 and AC × G 2333 were used to determine the position of the Co-AC locus in a 631 Kbp genomic region flanked by the SNP markers SS56 and SS92 on the lower arm of chromosome Pv01. By genotyping 77 F(3) plants from the AC × PI207262 cross using nine additional markers, we fine-mapped the Co-AC locus to a significantly smaller genomic region (9.4 Kbp) flanked by the SNP markers SS102 and SS165. This 9.4 Kbp region harbors three predicted genes based on the common bean reference genome, notably including the gene model Phvul.001G244300, which encodes Clathrin heavy chain 1, a protein that supports specific stomatal regulation functions and might play a role in plant defense signaling. Because the Co-AC resistance locus is linked in cis, it can be selected with great efficiency using molecular markers. These results will be very useful for breeding programs aimed at developing bean cultivars with anthracnose resistance using marker-assisted selection. This study revealed the broad-spectrum resistance of AC to C. lindemuthianum and the existence of the Co-AC anthracnose-resistance locus. Fine mapping positioned this locus in a small genomic region on the lower end of chromosome Pv01 that contained three candidate genes for the Co-AC locus. Public Library of Science 2020-10-07 /pmc/articles/PMC7540868/ /pubmed/33027258 http://dx.doi.org/10.1371/journal.pone.0239763 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Gilio, Thiago Alexandre Santana
Hurtado-Gonzales, Oscar P.
Gonçalves-Vidigal, Maria Celeste
Valentini, Giseli
Ferreira Elias, Julio Cesar
Song, Qijian
Pastor-Corrales, Marcial A.
Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo
title Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo
title_full Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo
title_fullStr Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo
title_full_unstemmed Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo
title_short Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo
title_sort fine mapping of an anthracnose-resistance locus in andean common bean cultivar amendoim cavalo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540868/
https://www.ncbi.nlm.nih.gov/pubmed/33027258
http://dx.doi.org/10.1371/journal.pone.0239763
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