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Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed

BACKGROUND: Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrop...

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Autores principales: Sun, Heng, Li, Juanjuan, Song, Heyun, Yang, Dong, Deng, Xianbao, Liu, Juan, Wang, Yunmeng, Ma, Junyu, Xiong, Yaqian, Liu, Yanling, Yang, Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541243/
https://www.ncbi.nlm.nih.gov/pubmed/33023477
http://dx.doi.org/10.1186/s12870-020-02666-z
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author Sun, Heng
Li, Juanjuan
Song, Heyun
Yang, Dong
Deng, Xianbao
Liu, Juan
Wang, Yunmeng
Ma, Junyu
Xiong, Yaqian
Liu, Yanling
Yang, Mei
author_facet Sun, Heng
Li, Juanjuan
Song, Heyun
Yang, Dong
Deng, Xianbao
Liu, Juan
Wang, Yunmeng
Ma, Junyu
Xiong, Yaqian
Liu, Yanling
Yang, Mei
author_sort Sun, Heng
collection PubMed
description BACKGROUND: Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. RESULTS: We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. CONCLUSIONS: Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.
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spelling pubmed-75412432020-10-08 Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed Sun, Heng Li, Juanjuan Song, Heyun Yang, Dong Deng, Xianbao Liu, Juan Wang, Yunmeng Ma, Junyu Xiong, Yaqian Liu, Yanling Yang, Mei BMC Plant Biol Research Article BACKGROUND: Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. RESULTS: We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. CONCLUSIONS: Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. BioMed Central 2020-10-06 /pmc/articles/PMC7541243/ /pubmed/33023477 http://dx.doi.org/10.1186/s12870-020-02666-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Sun, Heng
Li, Juanjuan
Song, Heyun
Yang, Dong
Deng, Xianbao
Liu, Juan
Wang, Yunmeng
Ma, Junyu
Xiong, Yaqian
Liu, Yanling
Yang, Mei
Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed
title Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed
title_full Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed
title_fullStr Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed
title_full_unstemmed Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed
title_short Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed
title_sort comprehensive analysis of agpase genes uncovers their potential roles in starch biosynthesis in lotus seed
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541243/
https://www.ncbi.nlm.nih.gov/pubmed/33023477
http://dx.doi.org/10.1186/s12870-020-02666-z
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