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Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In...

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Autores principales: Jiang, Yuan-Yuan, Chai, Yi-Ping, Lu, Min-Hui, Han, Xiu-Li, Lin, Qiupeng, Zhang, Yu, Zhang, Qiang, Zhou, Yun, Wang, Xue-Chen, Gao, Caixia, Chen, Qi-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541250/
https://www.ncbi.nlm.nih.gov/pubmed/33023639
http://dx.doi.org/10.1186/s13059-020-02170-5
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author Jiang, Yuan-Yuan
Chai, Yi-Ping
Lu, Min-Hui
Han, Xiu-Li
Lin, Qiupeng
Zhang, Yu
Zhang, Qiang
Zhou, Yun
Wang, Xue-Chen
Gao, Caixia
Chen, Qi-Jun
author_facet Jiang, Yuan-Yuan
Chai, Yi-Ping
Lu, Min-Hui
Han, Xiu-Li
Lin, Qiupeng
Zhang, Yu
Zhang, Qiang
Zhou, Yun
Wang, Xue-Chen
Gao, Caixia
Chen, Qi-Jun
author_sort Jiang, Yuan-Yuan
collection PubMed
description Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.
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spelling pubmed-75412502020-10-08 Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize Jiang, Yuan-Yuan Chai, Yi-Ping Lu, Min-Hui Han, Xiu-Li Lin, Qiupeng Zhang, Yu Zhang, Qiang Zhou, Yun Wang, Xue-Chen Gao, Caixia Chen, Qi-Jun Genome Biol Short Report Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors. BioMed Central 2020-10-06 /pmc/articles/PMC7541250/ /pubmed/33023639 http://dx.doi.org/10.1186/s13059-020-02170-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Jiang, Yuan-Yuan
Chai, Yi-Ping
Lu, Min-Hui
Han, Xiu-Li
Lin, Qiupeng
Zhang, Yu
Zhang, Qiang
Zhou, Yun
Wang, Xue-Chen
Gao, Caixia
Chen, Qi-Jun
Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
title Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
title_full Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
title_fullStr Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
title_full_unstemmed Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
title_short Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
title_sort prime editing efficiently generates w542l and s621i double mutations in two als genes in maize
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541250/
https://www.ncbi.nlm.nih.gov/pubmed/33023639
http://dx.doi.org/10.1186/s13059-020-02170-5
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