The murine Microenvironment Cell Population counter method to estimate abundance of tissue-infiltrating immune and stromal cell populations in murine samples using gene expression

Quantifying tissue-infiltrating immune and stromal cells provides clinically relevant information for various diseases. While numerous methods can quantify immune or stromal cells in human tissue samples from transcriptomic data, few are available for mouse studies. We introduce murine Microenvironm...

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Detalles Bibliográficos
Autores principales: Petitprez, Florent, Levy, Sacha, Sun, Cheng-Ming, Meylan, Maxime, Linhard, Christophe, Becht, Etienne, Elarouci, Nabila, Tavel, David, Roumenina, Lubka T., Ayadi, Mira, Sautès-Fridman, Catherine, Fridman, Wolf H., de Reyniès, Aurélien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541325/
https://www.ncbi.nlm.nih.gov/pubmed/33023656
http://dx.doi.org/10.1186/s13073-020-00783-w
Descripción
Sumario:Quantifying tissue-infiltrating immune and stromal cells provides clinically relevant information for various diseases. While numerous methods can quantify immune or stromal cells in human tissue samples from transcriptomic data, few are available for mouse studies. We introduce murine Microenvironment Cell Population counter (mMCP-counter), a method based on highly specific transcriptomic markers that accurately quantify 16 immune and stromal murine cell populations. We validated mMCP-counter with flow cytometry data and showed that mMCP-counter outperforms existing methods. We showed that mMCP-counter scores are predictive of response to immune checkpoint blockade in cancer mouse models and identify early immune impacts of Alzheimer’s disease.

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