Cargando…
Thallium-201 Imaging in Intact Olfactory Sensory Neurons with Reduced Pre-Synaptic Inhibition In Vivo
In this study, we determined whether the (201)Tl (thallium-201)-based olfactory imaging is affected if olfactory sensory neurons received reduced pre-synaptic inhibition signals from dopaminergic interneurons in the olfactory bulb in vivo. The thallium-201 migration rate to the olfactory bulb and th...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541386/ https://www.ncbi.nlm.nih.gov/pubmed/32820461 http://dx.doi.org/10.1007/s12035-020-02078-y |
_version_ | 1783591396387586048 |
---|---|
author | Shiga, Hideaki Wakabayashi, Hiroshi Washiyama, Kohshin Noguchi, Tomohiro Hiromasa, Tomo Miyazono, Sadaharu Kumai, Masami Ogawa, Kazuma Taki, Junichi Kinuya, Seigo Miwa, Takaki |
author_facet | Shiga, Hideaki Wakabayashi, Hiroshi Washiyama, Kohshin Noguchi, Tomohiro Hiromasa, Tomo Miyazono, Sadaharu Kumai, Masami Ogawa, Kazuma Taki, Junichi Kinuya, Seigo Miwa, Takaki |
author_sort | Shiga, Hideaki |
collection | PubMed |
description | In this study, we determined whether the (201)Tl (thallium-201)-based olfactory imaging is affected if olfactory sensory neurons received reduced pre-synaptic inhibition signals from dopaminergic interneurons in the olfactory bulb in vivo. The thallium-201 migration rate to the olfactory bulb and the number of action potentials of olfactory sensory neurons were assessed 3 h following left side nasal administration of rotenone, a mitochondrial respiratory chain complex I inhibitor that decreases the number of dopaminergic interneurons without damaging the olfactory sensory neurons in the olfactory bulb, in mice (6–7 animals per group). The migration rate of thallium-201 to the olfactory bulb was significantly increased following intranasal administration of thallium-201 and rotenone (10 μg rotenone, p = 0.0012; 20 μg rotenone, p = 0.0012), compared with that in control mice. The number of action potentials was significantly reduced in the olfactory sensory neurons in the rotenone treated side of 20 μg rotenone-treated mice, compared with that in control mice (p = 0.0029). The migration rate of thallium-201 to the olfactory bulb assessed with SPECT-CT was significantly increased in rats 24 h after the left intranasal administration of thallium-201 and 100 μg rotenone, compared with that in control rats (p = 0.008, 5 rats per group). Our results suggest that thallium-201 migration to the olfactory bulb is increased in intact olfactory sensory neurons with reduced pre-synaptic inhibition from dopaminergic interneurons in olfactory bulb glomeruli. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12035-020-02078-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7541386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-75413862020-10-19 Thallium-201 Imaging in Intact Olfactory Sensory Neurons with Reduced Pre-Synaptic Inhibition In Vivo Shiga, Hideaki Wakabayashi, Hiroshi Washiyama, Kohshin Noguchi, Tomohiro Hiromasa, Tomo Miyazono, Sadaharu Kumai, Masami Ogawa, Kazuma Taki, Junichi Kinuya, Seigo Miwa, Takaki Mol Neurobiol Article In this study, we determined whether the (201)Tl (thallium-201)-based olfactory imaging is affected if olfactory sensory neurons received reduced pre-synaptic inhibition signals from dopaminergic interneurons in the olfactory bulb in vivo. The thallium-201 migration rate to the olfactory bulb and the number of action potentials of olfactory sensory neurons were assessed 3 h following left side nasal administration of rotenone, a mitochondrial respiratory chain complex I inhibitor that decreases the number of dopaminergic interneurons without damaging the olfactory sensory neurons in the olfactory bulb, in mice (6–7 animals per group). The migration rate of thallium-201 to the olfactory bulb was significantly increased following intranasal administration of thallium-201 and rotenone (10 μg rotenone, p = 0.0012; 20 μg rotenone, p = 0.0012), compared with that in control mice. The number of action potentials was significantly reduced in the olfactory sensory neurons in the rotenone treated side of 20 μg rotenone-treated mice, compared with that in control mice (p = 0.0029). The migration rate of thallium-201 to the olfactory bulb assessed with SPECT-CT was significantly increased in rats 24 h after the left intranasal administration of thallium-201 and 100 μg rotenone, compared with that in control rats (p = 0.008, 5 rats per group). Our results suggest that thallium-201 migration to the olfactory bulb is increased in intact olfactory sensory neurons with reduced pre-synaptic inhibition from dopaminergic interneurons in olfactory bulb glomeruli. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12035-020-02078-y) contains supplementary material, which is available to authorized users. Springer US 2020-08-20 2020 /pmc/articles/PMC7541386/ /pubmed/32820461 http://dx.doi.org/10.1007/s12035-020-02078-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shiga, Hideaki Wakabayashi, Hiroshi Washiyama, Kohshin Noguchi, Tomohiro Hiromasa, Tomo Miyazono, Sadaharu Kumai, Masami Ogawa, Kazuma Taki, Junichi Kinuya, Seigo Miwa, Takaki Thallium-201 Imaging in Intact Olfactory Sensory Neurons with Reduced Pre-Synaptic Inhibition In Vivo |
title | Thallium-201 Imaging in Intact Olfactory Sensory Neurons with Reduced Pre-Synaptic Inhibition In Vivo |
title_full | Thallium-201 Imaging in Intact Olfactory Sensory Neurons with Reduced Pre-Synaptic Inhibition In Vivo |
title_fullStr | Thallium-201 Imaging in Intact Olfactory Sensory Neurons with Reduced Pre-Synaptic Inhibition In Vivo |
title_full_unstemmed | Thallium-201 Imaging in Intact Olfactory Sensory Neurons with Reduced Pre-Synaptic Inhibition In Vivo |
title_short | Thallium-201 Imaging in Intact Olfactory Sensory Neurons with Reduced Pre-Synaptic Inhibition In Vivo |
title_sort | thallium-201 imaging in intact olfactory sensory neurons with reduced pre-synaptic inhibition in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541386/ https://www.ncbi.nlm.nih.gov/pubmed/32820461 http://dx.doi.org/10.1007/s12035-020-02078-y |
work_keys_str_mv | AT shigahideaki thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo AT wakabayashihiroshi thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo AT washiyamakohshin thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo AT noguchitomohiro thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo AT hiromasatomo thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo AT miyazonosadaharu thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo AT kumaimasami thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo AT ogawakazuma thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo AT takijunichi thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo AT kinuyaseigo thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo AT miwatakaki thallium201imaginginintactolfactorysensoryneuronswithreducedpresynapticinhibitioninvivo |