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Putative biomarkers for early diagnosis and prognosis of congenital ocular toxoplasmosis

In the present study we have evaluated the performance of several immunological biomarkers for early diagnosis and prognosis of congenital toxoplasmosis. Our results showed that ex vivo serum levels of CXCL9, and the frequencies of circulating CD4(+)CD25(+) T-cells and T. gondii-specific IFN-γ(+)CD4...

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Detalles Bibliográficos
Autores principales: de Araújo, Thádia Evelyn, dos Santos, Luara Isabela, Gomes, Angelica Oliveira, Carneiro, Ana Carolina Aguiar Vasconcelos, Machado, Anderson Silva, Coelho-dos-Reis, Jordana Grazziela, Peruhype-Magalhães, Vanessa, Béla, Samantha Ribeiro, Andrade, Gláucia Manzan Queiroz, Vasconcelos-Santos, Daniel Vitor, Januário, José Nélio, Teixeira-Carvalho, Andréa, Vitor, Ricardo Wagner Almeida, do Valle Antonelli, Lis Ribeiro, Ferro, Eloisa Amália Vieira, Martins-Filho, Olindo Assis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541609/
https://www.ncbi.nlm.nih.gov/pubmed/33028847
http://dx.doi.org/10.1038/s41598-020-73265-z
Descripción
Sumario:In the present study we have evaluated the performance of several immunological biomarkers for early diagnosis and prognosis of congenital toxoplasmosis. Our results showed that ex vivo serum levels of CXCL9, and the frequencies of circulating CD4(+)CD25(+) T-cells and T. gondii-specific IFN-γ(+)CD4(+) T-cells measured 30–45 days after birth presented high accuracy to distinguish T. gondii-infected infants from healthy age-matched controls (Global Accuracy/AUC = 0.9; 0.9 and 0.8, respectively). Of note was the enhanced performance (Accuracy = 96%) achieved by using a combined stepwise analysis of CD4(+)CD25(+) T-cells and CXCL9. In addition, high global accuracy (AUC = 0.9) with elevated sensitivity (Se = 98%) was also reached by using the total frequency of in vitro IFN-γ-producing T. gondii-specific T-cells (∑ IFN-γ(+) CD4(+) & CD8(+)) as a biomarker of congenital toxoplasmosis. Furthermore, the analysis of in vitro T. gondii-specific IL5(+)CD4(+) T-cells and IFN-γ(+)NK-cells displayed a high accuracy for early prognosis of ocular lesion in infant with congenital toxoplasmosis (Global Accuracy/AUC = 0.8 and 0.9, respectively). Together, these findings support the relevance of employing the elements of the cell-mediated immune response as biomarkers with potential to endorse early diagnosis and prognosis of congenital ocular toxoplasmosis to contribute for a precise clinical management and effective therapeutic intervention.