Cargando…

Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides

The ascomycete fungus Colletotrichum gloeosporioides infects a wide range of plant hosts and causes enormous economic losses in the world. The transcription factors (TFs) play an important role in development and pathogenicity of many organisms. In this study, we found that the C(2)H(2) TF CgCrzA is...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Ping, Li, Bing, Pan, Yu-Ting, Zhang, Yun-Zhao, Li, De-Wei, Huang, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542025/
https://www.ncbi.nlm.nih.gov/pubmed/33082723
http://dx.doi.org/10.5423/PPJ.OA.04.2020.0071
_version_ 1783591475262521344
author Wang, Ping
Li, Bing
Pan, Yu-Ting
Zhang, Yun-Zhao
Li, De-Wei
Huang, Lin
author_facet Wang, Ping
Li, Bing
Pan, Yu-Ting
Zhang, Yun-Zhao
Li, De-Wei
Huang, Lin
author_sort Wang, Ping
collection PubMed
description The ascomycete fungus Colletotrichum gloeosporioides infects a wide range of plant hosts and causes enormous economic losses in the world. The transcription factors (TFs) play an important role in development and pathogenicity of many organisms. In this study, we found that the C(2)H(2) TF CgCrzA is localized in both cytoplasm and nucleus under standard condition, and it translocated from cytoplasm to nucleus in a calcineurin-dependent manner. Moreover, the ΔCgCrzA was hypersensitive to cell wall perturbing agents and showed severe cell wall integrity defects. Deletion of the CgCRZA inhibited the development of invasive structures and lost pathogenicity to plant hosts. Our results suggested that calcineurin-responsive TF CgCrzA was not only involved in regulating cell wall integrity, but also in morphogenesis and virulence in C. gloeosporioides.
format Online
Article
Text
id pubmed-7542025
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Korean Society of Plant Pathology
record_format MEDLINE/PubMed
spelling pubmed-75420252020-10-19 Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides Wang, Ping Li, Bing Pan, Yu-Ting Zhang, Yun-Zhao Li, De-Wei Huang, Lin Plant Pathol J Research Article The ascomycete fungus Colletotrichum gloeosporioides infects a wide range of plant hosts and causes enormous economic losses in the world. The transcription factors (TFs) play an important role in development and pathogenicity of many organisms. In this study, we found that the C(2)H(2) TF CgCrzA is localized in both cytoplasm and nucleus under standard condition, and it translocated from cytoplasm to nucleus in a calcineurin-dependent manner. Moreover, the ΔCgCrzA was hypersensitive to cell wall perturbing agents and showed severe cell wall integrity defects. Deletion of the CgCRZA inhibited the development of invasive structures and lost pathogenicity to plant hosts. Our results suggested that calcineurin-responsive TF CgCrzA was not only involved in regulating cell wall integrity, but also in morphogenesis and virulence in C. gloeosporioides. Korean Society of Plant Pathology 2020-10-01 2020-10-01 /pmc/articles/PMC7542025/ /pubmed/33082723 http://dx.doi.org/10.5423/PPJ.OA.04.2020.0071 Text en © The Korean Society of Plant Pathology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Ping
Li, Bing
Pan, Yu-Ting
Zhang, Yun-Zhao
Li, De-Wei
Huang, Lin
Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides
title Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides
title_full Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides
title_fullStr Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides
title_full_unstemmed Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides
title_short Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides
title_sort calcineurin-responsive transcription factor cgcrza is required for cell wall integrity and infection-related morphogenesis in colletotrichum gloeosporioides
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542025/
https://www.ncbi.nlm.nih.gov/pubmed/33082723
http://dx.doi.org/10.5423/PPJ.OA.04.2020.0071
work_keys_str_mv AT wangping calcineurinresponsivetranscriptionfactorcgcrzaisrequiredforcellwallintegrityandinfectionrelatedmorphogenesisincolletotrichumgloeosporioides
AT libing calcineurinresponsivetranscriptionfactorcgcrzaisrequiredforcellwallintegrityandinfectionrelatedmorphogenesisincolletotrichumgloeosporioides
AT panyuting calcineurinresponsivetranscriptionfactorcgcrzaisrequiredforcellwallintegrityandinfectionrelatedmorphogenesisincolletotrichumgloeosporioides
AT zhangyunzhao calcineurinresponsivetranscriptionfactorcgcrzaisrequiredforcellwallintegrityandinfectionrelatedmorphogenesisincolletotrichumgloeosporioides
AT lidewei calcineurinresponsivetranscriptionfactorcgcrzaisrequiredforcellwallintegrityandinfectionrelatedmorphogenesisincolletotrichumgloeosporioides
AT huanglin calcineurinresponsivetranscriptionfactorcgcrzaisrequiredforcellwallintegrityandinfectionrelatedmorphogenesisincolletotrichumgloeosporioides