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Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing
Malva vein clearing virus (MVCV) is a member of the Potyvirus species, and has a negative impact on the aesthetic development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society of Plant Pathology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542035/ https://www.ncbi.nlm.nih.gov/pubmed/33082731 http://dx.doi.org/10.5423/PPJ.OA.07.2020.0126 |
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author | Wang, Defu Cui, Liyan Pei, Yanni Ma, Zhennan Shen, Shaofei Long, Dandan Li, Lingyu Niu, Yanbing |
author_facet | Wang, Defu Cui, Liyan Pei, Yanni Ma, Zhennan Shen, Shaofei Long, Dandan Li, Lingyu Niu, Yanbing |
author_sort | Wang, Defu |
collection | PubMed |
description | Malva vein clearing virus (MVCV) is a member of the Potyvirus species, and has a negative impact on the aesthetic development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCV-SX) by specific primers targeting identified contig gaps, the full-length genome sequences (9,645 nucleotides) of MVCV-SX were characterized, constituting of an open reading frame that is long enough to encode 3,096 amino acids. Phylogenetic analysis showed that MVCV-SX was clustered with euphorbia ringspot virus and yam mosaic virus. Further analyses of the vsiR profiles revealed that the most abundant MVCV-vsiRs were between 21 and 22 nucleotides in length and a strong bias was found for “A” and “U” at the 5′-terminal residue. The results of polarity assessment indicated that the amount of sense strand was almost equal to that of the antisense strand in MVCV-vsiRs, and the main hot-spot region in MVCV-SX genome was found at cylindrical inclusion. In conclusion, our findings could provide new insights into the RNA silencing-mediated host defence mechanism in A. rosea infected with MVCV-SX, and offer a basis for the prevention and treatment of this virus disease. |
format | Online Article Text |
id | pubmed-7542035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Korean Society of Plant Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-75420352020-10-19 Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing Wang, Defu Cui, Liyan Pei, Yanni Ma, Zhennan Shen, Shaofei Long, Dandan Li, Lingyu Niu, Yanbing Plant Pathol J Research Article Malva vein clearing virus (MVCV) is a member of the Potyvirus species, and has a negative impact on the aesthetic development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCV-SX) by specific primers targeting identified contig gaps, the full-length genome sequences (9,645 nucleotides) of MVCV-SX were characterized, constituting of an open reading frame that is long enough to encode 3,096 amino acids. Phylogenetic analysis showed that MVCV-SX was clustered with euphorbia ringspot virus and yam mosaic virus. Further analyses of the vsiR profiles revealed that the most abundant MVCV-vsiRs were between 21 and 22 nucleotides in length and a strong bias was found for “A” and “U” at the 5′-terminal residue. The results of polarity assessment indicated that the amount of sense strand was almost equal to that of the antisense strand in MVCV-vsiRs, and the main hot-spot region in MVCV-SX genome was found at cylindrical inclusion. In conclusion, our findings could provide new insights into the RNA silencing-mediated host defence mechanism in A. rosea infected with MVCV-SX, and offer a basis for the prevention and treatment of this virus disease. Korean Society of Plant Pathology 2020-10-01 2020-10-01 /pmc/articles/PMC7542035/ /pubmed/33082731 http://dx.doi.org/10.5423/PPJ.OA.07.2020.0126 Text en © The Korean Society of Plant Pathology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Defu Cui, Liyan Pei, Yanni Ma, Zhennan Shen, Shaofei Long, Dandan Li, Lingyu Niu, Yanbing Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing |
title | Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing |
title_full | Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing |
title_fullStr | Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing |
title_full_unstemmed | Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing |
title_short | Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing |
title_sort | characterization of a strain of malva vein clearing virus in alcea rosea via deep sequencing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542035/ https://www.ncbi.nlm.nih.gov/pubmed/33082731 http://dx.doi.org/10.5423/PPJ.OA.07.2020.0126 |
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