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Evaluation of indomethacin as matrix metalloproteases inhibitor in human dentin

OBJECTIVE: The objective was to determine a new experimental material, indomethacin's inhibitory effect on the enzymatic activity of dentin collagen. MATERIALS AND METHODS: Fifteen freshly extracted teeth were collected and stored at 4°C until use. Enamel, roots, and remnant pulp tissue were re...

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Autores principales: Shailendra, Mashalkar, Bhandari, Sarita, Kulkarni, Sangeeta, Janavathi, K., Ghatole, Kiran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542069/
https://www.ncbi.nlm.nih.gov/pubmed/33088073
http://dx.doi.org/10.4103/JCD.JCD_236_19
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author Shailendra, Mashalkar
Bhandari, Sarita
Kulkarni, Sangeeta
Janavathi, K.
Ghatole, Kiran
author_facet Shailendra, Mashalkar
Bhandari, Sarita
Kulkarni, Sangeeta
Janavathi, K.
Ghatole, Kiran
author_sort Shailendra, Mashalkar
collection PubMed
description OBJECTIVE: The objective was to determine a new experimental material, indomethacin's inhibitory effect on the enzymatic activity of dentin collagen. MATERIALS AND METHODS: Fifteen freshly extracted teeth were collected and stored at 4°C until use. Enamel, roots, and remnant pulp tissue were removed, and dentin powder was obtained by pulverizing liquid nitrogen-frozen coronal dentin with a mortar pestle. The obtained protein extract from human dentin powder was treated with indomethacin and incubated. The inhibition of enzymatic activity was analyzed using plate assay method and zymographic analysis. RESULTS: Plate assay method and zymograms showed that indomethacin-treated samples inhibited dentin enzymatic activity. SIGNIFICANCE: Bond strength at the dentin adhesive interface decreases because of the hydrolytic degradation of dentin collagen. The inhibition of enzymes responsible for collagen degradation may improve the bond strength durability. This study demonstrates the efficacy of indomethacin in inhibiting enzymatic activity.
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spelling pubmed-75420692020-10-20 Evaluation of indomethacin as matrix metalloproteases inhibitor in human dentin Shailendra, Mashalkar Bhandari, Sarita Kulkarni, Sangeeta Janavathi, K. Ghatole, Kiran J Conserv Dent Original Article OBJECTIVE: The objective was to determine a new experimental material, indomethacin's inhibitory effect on the enzymatic activity of dentin collagen. MATERIALS AND METHODS: Fifteen freshly extracted teeth were collected and stored at 4°C until use. Enamel, roots, and remnant pulp tissue were removed, and dentin powder was obtained by pulverizing liquid nitrogen-frozen coronal dentin with a mortar pestle. The obtained protein extract from human dentin powder was treated with indomethacin and incubated. The inhibition of enzymatic activity was analyzed using plate assay method and zymographic analysis. RESULTS: Plate assay method and zymograms showed that indomethacin-treated samples inhibited dentin enzymatic activity. SIGNIFICANCE: Bond strength at the dentin adhesive interface decreases because of the hydrolytic degradation of dentin collagen. The inhibition of enzymes responsible for collagen degradation may improve the bond strength durability. This study demonstrates the efficacy of indomethacin in inhibiting enzymatic activity. Wolters Kluwer - Medknow 2019 2020-08-20 /pmc/articles/PMC7542069/ /pubmed/33088073 http://dx.doi.org/10.4103/JCD.JCD_236_19 Text en Copyright: © 2020 Journal of Conservative Dentistry http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Shailendra, Mashalkar
Bhandari, Sarita
Kulkarni, Sangeeta
Janavathi, K.
Ghatole, Kiran
Evaluation of indomethacin as matrix metalloproteases inhibitor in human dentin
title Evaluation of indomethacin as matrix metalloproteases inhibitor in human dentin
title_full Evaluation of indomethacin as matrix metalloproteases inhibitor in human dentin
title_fullStr Evaluation of indomethacin as matrix metalloproteases inhibitor in human dentin
title_full_unstemmed Evaluation of indomethacin as matrix metalloproteases inhibitor in human dentin
title_short Evaluation of indomethacin as matrix metalloproteases inhibitor in human dentin
title_sort evaluation of indomethacin as matrix metalloproteases inhibitor in human dentin
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542069/
https://www.ncbi.nlm.nih.gov/pubmed/33088073
http://dx.doi.org/10.4103/JCD.JCD_236_19
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