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Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance

OBJECTIVE: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplification of 20 heterologous m...

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Autores principales: Gallardo, Franco F. Roldán, DeMatteo, Karen E., Rinas, Miguel A., Argüelles, Carina F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542955/
https://www.ncbi.nlm.nih.gov/pubmed/33028373
http://dx.doi.org/10.1186/s13104-020-05312-x
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author Gallardo, Franco F. Roldán
DeMatteo, Karen E.
Rinas, Miguel A.
Argüelles, Carina F.
author_facet Gallardo, Franco F. Roldán
DeMatteo, Karen E.
Rinas, Miguel A.
Argüelles, Carina F.
author_sort Gallardo, Franco F. Roldán
collection PubMed
description OBJECTIVE: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplification of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). RESULTS: Those primers that showed the best amplification profile in blood samples were subsequently applied to scats and saliva samples, to evaluate their efficiency. Of the 13 microsatellite pairs that amplified in blood, one-third (32%) were successfully amplified in saliva and scat samples. This initial work demonstrates successful cross-amplification in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade.
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spelling pubmed-75429552020-10-13 Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance Gallardo, Franco F. Roldán DeMatteo, Karen E. Rinas, Miguel A. Argüelles, Carina F. BMC Res Notes Research Note OBJECTIVE: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplification of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). RESULTS: Those primers that showed the best amplification profile in blood samples were subsequently applied to scats and saliva samples, to evaluate their efficiency. Of the 13 microsatellite pairs that amplified in blood, one-third (32%) were successfully amplified in saliva and scat samples. This initial work demonstrates successful cross-amplification in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade. BioMed Central 2020-10-07 /pmc/articles/PMC7542955/ /pubmed/33028373 http://dx.doi.org/10.1186/s13104-020-05312-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Gallardo, Franco F. Roldán
DeMatteo, Karen E.
Rinas, Miguel A.
Argüelles, Carina F.
Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title_full Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title_fullStr Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title_full_unstemmed Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title_short Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title_sort heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542955/
https://www.ncbi.nlm.nih.gov/pubmed/33028373
http://dx.doi.org/10.1186/s13104-020-05312-x
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