Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes

Isolation of the new pandemic virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for diagnostic and research purposes including assessment of novel therapeutics. Several primary and continuous cell lines are currently used, and new organoid and engineered cell lines are...

Descripción completa

Detalles Bibliográficos
Autores principales: Stelzer-Braid, Sacha, Walker, Gregory J., Aggarwal, Anupriya, Isaacs, Sonia R., Yeang, Malinna, Naing, Zin, Ospina Stella, Alberto, Turville, Stuart G., Rawlinson, William D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. on behalf of Royal College of Pathologists of Australasia. 2020
Materias:
FOCUS ON SARS-CoV-2
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7543926/
https://www.ncbi.nlm.nih.gov/pubmed/33131800
http://dx.doi.org/10.1016/j.pathol.2020.09.012
_version_ 1783591754551787520
author Stelzer-Braid, Sacha
Walker, Gregory J.
Aggarwal, Anupriya
Isaacs, Sonia R.
Yeang, Malinna
Naing, Zin
Ospina Stella, Alberto
Turville, Stuart G.
Rawlinson, William D.
author_facet Stelzer-Braid, Sacha
Walker, Gregory J.
Aggarwal, Anupriya
Isaacs, Sonia R.
Yeang, Malinna
Naing, Zin
Ospina Stella, Alberto
Turville, Stuart G.
Rawlinson, William D.
author_sort Stelzer-Braid, Sacha
collection PubMed
description Isolation of the new pandemic virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for diagnostic and research purposes including assessment of novel therapeutics. Several primary and continuous cell lines are currently used, and new organoid and engineered cell lines are being developed for improved investigation and understanding of the human immune response to this virus. Here we review the growth of SARS-CoV-2 in reference standard cell lines, engineered cell lines and new developments in this field.
format Online
Article
Text
id pubmed-7543926
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Published by Elsevier B.V. on behalf of Royal College of Pathologists of Australasia.
record_format MEDLINE/PubMed
spelling pubmed-75439262020-10-09 Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes Stelzer-Braid, Sacha Walker, Gregory J. Aggarwal, Anupriya Isaacs, Sonia R. Yeang, Malinna Naing, Zin Ospina Stella, Alberto Turville, Stuart G. Rawlinson, William D. Pathology FOCUS ON SARS-CoV-2 Isolation of the new pandemic virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for diagnostic and research purposes including assessment of novel therapeutics. Several primary and continuous cell lines are currently used, and new organoid and engineered cell lines are being developed for improved investigation and understanding of the human immune response to this virus. Here we review the growth of SARS-CoV-2 in reference standard cell lines, engineered cell lines and new developments in this field. Published by Elsevier B.V. on behalf of Royal College of Pathologists of Australasia. 2020-12 2020-10-08 /pmc/articles/PMC7543926/ /pubmed/33131800 http://dx.doi.org/10.1016/j.pathol.2020.09.012 Text en © 2020 Published by Elsevier B.V. on behalf of Royal College of Pathologists of Australasia. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle FOCUS ON SARS-CoV-2
Stelzer-Braid, Sacha
Walker, Gregory J.
Aggarwal, Anupriya
Isaacs, Sonia R.
Yeang, Malinna
Naing, Zin
Ospina Stella, Alberto
Turville, Stuart G.
Rawlinson, William D.
Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes
title Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes
title_full Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes
title_fullStr Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes
title_full_unstemmed Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes
title_short Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes
title_sort virus isolation of severe acute respiratory syndrome coronavirus 2 (sars-cov-2) for diagnostic and research purposes
topic FOCUS ON SARS-CoV-2
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7543926/
https://www.ncbi.nlm.nih.gov/pubmed/33131800
http://dx.doi.org/10.1016/j.pathol.2020.09.012
work_keys_str_mv AT stelzerbraidsacha virusisolationofsevereacuterespiratorysyndromecoronavirus2sarscov2fordiagnosticandresearchpurposes
AT walkergregoryj virusisolationofsevereacuterespiratorysyndromecoronavirus2sarscov2fordiagnosticandresearchpurposes
AT aggarwalanupriya virusisolationofsevereacuterespiratorysyndromecoronavirus2sarscov2fordiagnosticandresearchpurposes
AT isaacssoniar virusisolationofsevereacuterespiratorysyndromecoronavirus2sarscov2fordiagnosticandresearchpurposes
AT yeangmalinna virusisolationofsevereacuterespiratorysyndromecoronavirus2sarscov2fordiagnosticandresearchpurposes
AT naingzin virusisolationofsevereacuterespiratorysyndromecoronavirus2sarscov2fordiagnosticandresearchpurposes
AT ospinastellaalberto virusisolationofsevereacuterespiratorysyndromecoronavirus2sarscov2fordiagnosticandresearchpurposes
AT turvillestuartg virusisolationofsevereacuterespiratorysyndromecoronavirus2sarscov2fordiagnosticandresearchpurposes
AT rawlinsonwilliamd virusisolationofsevereacuterespiratorysyndromecoronavirus2sarscov2fordiagnosticandresearchpurposes

Ejemplares similares