Enhancing site-specific DNA integration by a Cas9 nuclease fused with a DNA donor-binding domain

The CRISPR/Cas system is widely used for genome editing. However, robust and targeted insertion of a DNA segment remains a challenge. Here, we present a fusion nuclease (Cas9-N57) to enhance site-specific DNA integration via a fused DNA binding domain of Sleeping Beauty transposase to tether the DNA...

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Detalles Bibliográficos
Autores principales: Ma, Shufeng, Wang, Xinlong, Hu, Yongfei, Lv, Jie, Liu, Chengfang, Liao, Kaitong, Guo, Xiaohua, Wang, Dong, Lin, Ying, Rong, Zhili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544211/
https://www.ncbi.nlm.nih.gov/pubmed/32986839
http://dx.doi.org/10.1093/nar/gkaa779

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