Cargando…

Chemical-neuroanatomical organization of peripheral sensory-efferent systems in the pond snail (Lymnaea stagnalis)

Perception and processing of chemical cues are crucial for aquatic gastropods, for proper elaboration of adaptive behavior. The pond snail, Lymnaea stagnalis, is a model species of invertebrate neurobiology, in which peripheral sensory neurons with different morphology and transmitter content have p...

Descripción completa

Detalles Bibliográficos
Autores principales: Horváth, Réka, Battonyai, Izabella, Maász, Gábor, Schmidt, János, Fekete, Zsuzsanna N., Elekes, Károly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544616/
https://www.ncbi.nlm.nih.gov/pubmed/32951073
http://dx.doi.org/10.1007/s00429-020-02145-z
_version_ 1783591885756956672
author Horváth, Réka
Battonyai, Izabella
Maász, Gábor
Schmidt, János
Fekete, Zsuzsanna N.
Elekes, Károly
author_facet Horváth, Réka
Battonyai, Izabella
Maász, Gábor
Schmidt, János
Fekete, Zsuzsanna N.
Elekes, Károly
author_sort Horváth, Réka
collection PubMed
description Perception and processing of chemical cues are crucial for aquatic gastropods, for proper elaboration of adaptive behavior. The pond snail, Lymnaea stagnalis, is a model species of invertebrate neurobiology, in which peripheral sensory neurons with different morphology and transmitter content have partly been described, but we have little knowledge regarding their functional morphological organization, including their possible peripheral intercellular connections and networks. Therefore the aim of our study was to characterize the sensory system of the tentacles and the lip, as primary sensory regions, and the anterior foot of Lymnaea with special attention to the transmitter content of the sensory neurons, and their relationship to extrinsic elements of the central nervous system. Numerous bipolar sensory cells were demonstrated in the epithelial layer of the peripheral organs, displaying immunoreactivity to antibodies raised against tyrosine hydroxylase, histamine, glutamate and two molluscan type oligopeptides, FMRFamide and Mytilus inhibitory peptide. A subepithelial plexus was formed by extrinsic serotonin and FMRFamide immunoreactive fibers, whereas in deeper regions axon processess of different origin with various immunoreactivities formed networks, too. HPLC–MS assay confirmed the presence of the low molecular weight signal molecules in the three examined areas. Following double-labeling immunohistochemistry, close arrangements were observed, formed by sensory neurons and extrinsic serotonergic (and FMRFamidergic) fibers at axo-dendritic, axo-somatic and axo-axonic levels. Our results suggest the involvement of a much wider repertoire of signal molecules in peripheral sensory processes of Lymnaea, which can locally be modified by central input, hence influencing directly the responses to environmental cues.
format Online
Article
Text
id pubmed-7544616
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-75446162020-10-19 Chemical-neuroanatomical organization of peripheral sensory-efferent systems in the pond snail (Lymnaea stagnalis) Horváth, Réka Battonyai, Izabella Maász, Gábor Schmidt, János Fekete, Zsuzsanna N. Elekes, Károly Brain Struct Funct Original Article Perception and processing of chemical cues are crucial for aquatic gastropods, for proper elaboration of adaptive behavior. The pond snail, Lymnaea stagnalis, is a model species of invertebrate neurobiology, in which peripheral sensory neurons with different morphology and transmitter content have partly been described, but we have little knowledge regarding their functional morphological organization, including their possible peripheral intercellular connections and networks. Therefore the aim of our study was to characterize the sensory system of the tentacles and the lip, as primary sensory regions, and the anterior foot of Lymnaea with special attention to the transmitter content of the sensory neurons, and their relationship to extrinsic elements of the central nervous system. Numerous bipolar sensory cells were demonstrated in the epithelial layer of the peripheral organs, displaying immunoreactivity to antibodies raised against tyrosine hydroxylase, histamine, glutamate and two molluscan type oligopeptides, FMRFamide and Mytilus inhibitory peptide. A subepithelial plexus was formed by extrinsic serotonin and FMRFamide immunoreactive fibers, whereas in deeper regions axon processess of different origin with various immunoreactivities formed networks, too. HPLC–MS assay confirmed the presence of the low molecular weight signal molecules in the three examined areas. Following double-labeling immunohistochemistry, close arrangements were observed, formed by sensory neurons and extrinsic serotonergic (and FMRFamidergic) fibers at axo-dendritic, axo-somatic and axo-axonic levels. Our results suggest the involvement of a much wider repertoire of signal molecules in peripheral sensory processes of Lymnaea, which can locally be modified by central input, hence influencing directly the responses to environmental cues. Springer Berlin Heidelberg 2020-09-20 2020 /pmc/articles/PMC7544616/ /pubmed/32951073 http://dx.doi.org/10.1007/s00429-020-02145-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Horváth, Réka
Battonyai, Izabella
Maász, Gábor
Schmidt, János
Fekete, Zsuzsanna N.
Elekes, Károly
Chemical-neuroanatomical organization of peripheral sensory-efferent systems in the pond snail (Lymnaea stagnalis)
title Chemical-neuroanatomical organization of peripheral sensory-efferent systems in the pond snail (Lymnaea stagnalis)
title_full Chemical-neuroanatomical organization of peripheral sensory-efferent systems in the pond snail (Lymnaea stagnalis)
title_fullStr Chemical-neuroanatomical organization of peripheral sensory-efferent systems in the pond snail (Lymnaea stagnalis)
title_full_unstemmed Chemical-neuroanatomical organization of peripheral sensory-efferent systems in the pond snail (Lymnaea stagnalis)
title_short Chemical-neuroanatomical organization of peripheral sensory-efferent systems in the pond snail (Lymnaea stagnalis)
title_sort chemical-neuroanatomical organization of peripheral sensory-efferent systems in the pond snail (lymnaea stagnalis)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544616/
https://www.ncbi.nlm.nih.gov/pubmed/32951073
http://dx.doi.org/10.1007/s00429-020-02145-z
work_keys_str_mv AT horvathreka chemicalneuroanatomicalorganizationofperipheralsensoryefferentsystemsinthepondsnaillymnaeastagnalis
AT battonyaiizabella chemicalneuroanatomicalorganizationofperipheralsensoryefferentsystemsinthepondsnaillymnaeastagnalis
AT maaszgabor chemicalneuroanatomicalorganizationofperipheralsensoryefferentsystemsinthepondsnaillymnaeastagnalis
AT schmidtjanos chemicalneuroanatomicalorganizationofperipheralsensoryefferentsystemsinthepondsnaillymnaeastagnalis
AT feketezsuzsannan chemicalneuroanatomicalorganizationofperipheralsensoryefferentsystemsinthepondsnaillymnaeastagnalis
AT elekeskaroly chemicalneuroanatomicalorganizationofperipheralsensoryefferentsystemsinthepondsnaillymnaeastagnalis