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Long Non-coding RNA HOTAIR Function as a Competing Endogenous RNA for miR-149-5p to Promote the Cell Growth, Migration, and Invasion in Non-small Cell Lung Cancer

Lung cancer is a leading cause of cancer death all around the world. Long non-coding RNAs (lncRNAs) have been confirmed to be involved in carcinogenesis of malignancies. However, the molecular mechanism of most lncRNAs in various kinds of cancers remains unclear. LncRNA HOTAIR and HNRNPA1 are report...

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Autores principales: Li, Hang, Cui, Zhigang, Lv, Xiaoting, Li, Juan, Gao, Min, Yang, Zitai, Bi, Yanhong, Zhang, Ziwei, Wang, Shengli, Li, Sixuan, Zhou, Baosen, Yin, Zhihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545358/
https://www.ncbi.nlm.nih.gov/pubmed/33102210
http://dx.doi.org/10.3389/fonc.2020.528520
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author Li, Hang
Cui, Zhigang
Lv, Xiaoting
Li, Juan
Gao, Min
Yang, Zitai
Bi, Yanhong
Zhang, Ziwei
Wang, Shengli
Li, Sixuan
Zhou, Baosen
Yin, Zhihua
author_facet Li, Hang
Cui, Zhigang
Lv, Xiaoting
Li, Juan
Gao, Min
Yang, Zitai
Bi, Yanhong
Zhang, Ziwei
Wang, Shengli
Li, Sixuan
Zhou, Baosen
Yin, Zhihua
author_sort Li, Hang
collection PubMed
description Lung cancer is a leading cause of cancer death all around the world. Long non-coding RNAs (lncRNAs) have been confirmed to be involved in carcinogenesis of malignancies. However, the molecular mechanism of most lncRNAs in various kinds of cancers remains unclear. LncRNA HOTAIR and HNRNPA1 are reported to play an oncogenic role in non-small cell lung cancer, and the overexpression of HNRNPA1 is shown to promote the proliferation of lung adenocarcinoma cells. In our study, we find that the overexpression of HOTAIR could promote the proliferation and overexpression of miR-149-5p could inhibit the proliferation of lung cancer cells. Flow cytometric analysis determines that overexpression of miR-149-5p induces cell cycle arrest in the G0/G1 phases, whereas overexpression of HOTAIR decreases the proportion of G0/G1phase cells. Also, overexpression of HOTAIR promotes the migration and invasion ability of lung cancer cells, confirmed by the wound-healing and transwell assays, which are suppressed by overexpression of miR-149-5p. Furthermore, the dual-luciferase reporter assay indicates that miR-149-5p could bind both HOTAIR and the 3′UTR of HNRNPA1. In summary, we find that HOTAIR can regulate HNRNPA1 expression through a ceRNA mechanism by sequester miR-149-5p, which post-transcriptionally targets HNRNPA1, thus promoting lung cancer progression.
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spelling pubmed-75453582020-10-22 Long Non-coding RNA HOTAIR Function as a Competing Endogenous RNA for miR-149-5p to Promote the Cell Growth, Migration, and Invasion in Non-small Cell Lung Cancer Li, Hang Cui, Zhigang Lv, Xiaoting Li, Juan Gao, Min Yang, Zitai Bi, Yanhong Zhang, Ziwei Wang, Shengli Li, Sixuan Zhou, Baosen Yin, Zhihua Front Oncol Oncology Lung cancer is a leading cause of cancer death all around the world. Long non-coding RNAs (lncRNAs) have been confirmed to be involved in carcinogenesis of malignancies. However, the molecular mechanism of most lncRNAs in various kinds of cancers remains unclear. LncRNA HOTAIR and HNRNPA1 are reported to play an oncogenic role in non-small cell lung cancer, and the overexpression of HNRNPA1 is shown to promote the proliferation of lung adenocarcinoma cells. In our study, we find that the overexpression of HOTAIR could promote the proliferation and overexpression of miR-149-5p could inhibit the proliferation of lung cancer cells. Flow cytometric analysis determines that overexpression of miR-149-5p induces cell cycle arrest in the G0/G1 phases, whereas overexpression of HOTAIR decreases the proportion of G0/G1phase cells. Also, overexpression of HOTAIR promotes the migration and invasion ability of lung cancer cells, confirmed by the wound-healing and transwell assays, which are suppressed by overexpression of miR-149-5p. Furthermore, the dual-luciferase reporter assay indicates that miR-149-5p could bind both HOTAIR and the 3′UTR of HNRNPA1. In summary, we find that HOTAIR can regulate HNRNPA1 expression through a ceRNA mechanism by sequester miR-149-5p, which post-transcriptionally targets HNRNPA1, thus promoting lung cancer progression. Frontiers Media S.A. 2020-09-25 /pmc/articles/PMC7545358/ /pubmed/33102210 http://dx.doi.org/10.3389/fonc.2020.528520 Text en Copyright © 2020 Li, Cui, Lv, Li, Gao, Yang, Bi, Zhang, Wang, Li, Zhou and Yin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Li, Hang
Cui, Zhigang
Lv, Xiaoting
Li, Juan
Gao, Min
Yang, Zitai
Bi, Yanhong
Zhang, Ziwei
Wang, Shengli
Li, Sixuan
Zhou, Baosen
Yin, Zhihua
Long Non-coding RNA HOTAIR Function as a Competing Endogenous RNA for miR-149-5p to Promote the Cell Growth, Migration, and Invasion in Non-small Cell Lung Cancer
title Long Non-coding RNA HOTAIR Function as a Competing Endogenous RNA for miR-149-5p to Promote the Cell Growth, Migration, and Invasion in Non-small Cell Lung Cancer
title_full Long Non-coding RNA HOTAIR Function as a Competing Endogenous RNA for miR-149-5p to Promote the Cell Growth, Migration, and Invasion in Non-small Cell Lung Cancer
title_fullStr Long Non-coding RNA HOTAIR Function as a Competing Endogenous RNA for miR-149-5p to Promote the Cell Growth, Migration, and Invasion in Non-small Cell Lung Cancer
title_full_unstemmed Long Non-coding RNA HOTAIR Function as a Competing Endogenous RNA for miR-149-5p to Promote the Cell Growth, Migration, and Invasion in Non-small Cell Lung Cancer
title_short Long Non-coding RNA HOTAIR Function as a Competing Endogenous RNA for miR-149-5p to Promote the Cell Growth, Migration, and Invasion in Non-small Cell Lung Cancer
title_sort long non-coding rna hotair function as a competing endogenous rna for mir-149-5p to promote the cell growth, migration, and invasion in non-small cell lung cancer
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545358/
https://www.ncbi.nlm.nih.gov/pubmed/33102210
http://dx.doi.org/10.3389/fonc.2020.528520
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