The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease

A detailed description of pathophysiological effects that viruses exert on their host is still challenging. For the first time, we report a highly controllable viral expression model based on an iPS-cell line from a healthy human donor. The established viral model system enables a dose-dependent and...

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Autores principales: Peischard, Stefan, Ho, Huyen Tran, Piccini, Ilaria, Strutz-Seebohm, Nathalie, Röpke, Albrecht, Liashkovich, Ivan, Gosain, Hiteshika, Rieger, Bettina, Klingel, Karin, Eggers, Britta, Marcus, Katrin, Linke, Wolfgang A., Müller, Frank Ulrich, Ludwig, Stephan, Greber, Boris, Busch, Karin, Seebohm, Guiscard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546621/
https://www.ncbi.nlm.nih.gov/pubmed/33033381
http://dx.doi.org/10.1038/s41598-020-72966-9
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author Peischard, Stefan
Ho, Huyen Tran
Piccini, Ilaria
Strutz-Seebohm, Nathalie
Röpke, Albrecht
Liashkovich, Ivan
Gosain, Hiteshika
Rieger, Bettina
Klingel, Karin
Eggers, Britta
Marcus, Katrin
Linke, Wolfgang A.
Müller, Frank Ulrich
Ludwig, Stephan
Greber, Boris
Busch, Karin
Seebohm, Guiscard
author_facet Peischard, Stefan
Ho, Huyen Tran
Piccini, Ilaria
Strutz-Seebohm, Nathalie
Röpke, Albrecht
Liashkovich, Ivan
Gosain, Hiteshika
Rieger, Bettina
Klingel, Karin
Eggers, Britta
Marcus, Katrin
Linke, Wolfgang A.
Müller, Frank Ulrich
Ludwig, Stephan
Greber, Boris
Busch, Karin
Seebohm, Guiscard
author_sort Peischard, Stefan
collection PubMed
description A detailed description of pathophysiological effects that viruses exert on their host is still challenging. For the first time, we report a highly controllable viral expression model based on an iPS-cell line from a healthy human donor. The established viral model system enables a dose-dependent and highly localized RNA-virus expression in a fully controllable environment, giving rise for new applications for the scientific community.
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spelling pubmed-75466212020-10-14 The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease Peischard, Stefan Ho, Huyen Tran Piccini, Ilaria Strutz-Seebohm, Nathalie Röpke, Albrecht Liashkovich, Ivan Gosain, Hiteshika Rieger, Bettina Klingel, Karin Eggers, Britta Marcus, Katrin Linke, Wolfgang A. Müller, Frank Ulrich Ludwig, Stephan Greber, Boris Busch, Karin Seebohm, Guiscard Sci Rep Article A detailed description of pathophysiological effects that viruses exert on their host is still challenging. For the first time, we report a highly controllable viral expression model based on an iPS-cell line from a healthy human donor. The established viral model system enables a dose-dependent and highly localized RNA-virus expression in a fully controllable environment, giving rise for new applications for the scientific community. Nature Publishing Group UK 2020-10-08 /pmc/articles/PMC7546621/ /pubmed/33033381 http://dx.doi.org/10.1038/s41598-020-72966-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Peischard, Stefan
Ho, Huyen Tran
Piccini, Ilaria
Strutz-Seebohm, Nathalie
Röpke, Albrecht
Liashkovich, Ivan
Gosain, Hiteshika
Rieger, Bettina
Klingel, Karin
Eggers, Britta
Marcus, Katrin
Linke, Wolfgang A.
Müller, Frank Ulrich
Ludwig, Stephan
Greber, Boris
Busch, Karin
Seebohm, Guiscard
The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease
title The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease
title_full The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease
title_fullStr The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease
title_full_unstemmed The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease
title_short The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease
title_sort first versatile human ipsc-based model of ectopic virus induction allows new insights in rna-virus disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546621/
https://www.ncbi.nlm.nih.gov/pubmed/33033381
http://dx.doi.org/10.1038/s41598-020-72966-9
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