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Genome-wide chromatin accessibility is restricted by ANP32E
Genome-wide chromatin state underlies gene expression potential and cellular function. Epigenetic features and nucleosome positioning contribute to the accessibility of DNA, but widespread regulators of chromatin state are largely unknown. Our study investigates how coordination of ANP32E and H2A.Z...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546623/ https://www.ncbi.nlm.nih.gov/pubmed/33033242 http://dx.doi.org/10.1038/s41467-020-18821-x |
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author | Murphy, Kristin E. Meng, Fanju W. Makowski, Claire E. Murphy, Patrick J. |
author_facet | Murphy, Kristin E. Meng, Fanju W. Makowski, Claire E. Murphy, Patrick J. |
author_sort | Murphy, Kristin E. |
collection | PubMed |
description | Genome-wide chromatin state underlies gene expression potential and cellular function. Epigenetic features and nucleosome positioning contribute to the accessibility of DNA, but widespread regulators of chromatin state are largely unknown. Our study investigates how coordination of ANP32E and H2A.Z contributes to genome-wide chromatin state in mouse fibroblasts. We define H2A.Z as a universal chromatin accessibility factor, and demonstrate that ANP32E antagonizes H2A.Z accumulation to restrict chromatin accessibility genome-wide. In the absence of ANP32E, H2A.Z accumulates at promoters in a hierarchical manner. H2A.Z initially localizes downstream of the transcription start site, and if H2A.Z is already present downstream, additional H2A.Z accumulates upstream. This hierarchical H2A.Z accumulation coincides with improved nucleosome positioning, heightened transcription factor binding, and increased expression of neighboring genes. Thus, ANP32E dramatically influences genome-wide chromatin accessibility through subtle refinement of H2A.Z patterns, providing a means to reprogram chromatin state and to hone gene expression levels. |
format | Online Article Text |
id | pubmed-7546623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75466232020-10-19 Genome-wide chromatin accessibility is restricted by ANP32E Murphy, Kristin E. Meng, Fanju W. Makowski, Claire E. Murphy, Patrick J. Nat Commun Article Genome-wide chromatin state underlies gene expression potential and cellular function. Epigenetic features and nucleosome positioning contribute to the accessibility of DNA, but widespread regulators of chromatin state are largely unknown. Our study investigates how coordination of ANP32E and H2A.Z contributes to genome-wide chromatin state in mouse fibroblasts. We define H2A.Z as a universal chromatin accessibility factor, and demonstrate that ANP32E antagonizes H2A.Z accumulation to restrict chromatin accessibility genome-wide. In the absence of ANP32E, H2A.Z accumulates at promoters in a hierarchical manner. H2A.Z initially localizes downstream of the transcription start site, and if H2A.Z is already present downstream, additional H2A.Z accumulates upstream. This hierarchical H2A.Z accumulation coincides with improved nucleosome positioning, heightened transcription factor binding, and increased expression of neighboring genes. Thus, ANP32E dramatically influences genome-wide chromatin accessibility through subtle refinement of H2A.Z patterns, providing a means to reprogram chromatin state and to hone gene expression levels. Nature Publishing Group UK 2020-10-08 /pmc/articles/PMC7546623/ /pubmed/33033242 http://dx.doi.org/10.1038/s41467-020-18821-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Murphy, Kristin E. Meng, Fanju W. Makowski, Claire E. Murphy, Patrick J. Genome-wide chromatin accessibility is restricted by ANP32E |
title | Genome-wide chromatin accessibility is restricted by ANP32E |
title_full | Genome-wide chromatin accessibility is restricted by ANP32E |
title_fullStr | Genome-wide chromatin accessibility is restricted by ANP32E |
title_full_unstemmed | Genome-wide chromatin accessibility is restricted by ANP32E |
title_short | Genome-wide chromatin accessibility is restricted by ANP32E |
title_sort | genome-wide chromatin accessibility is restricted by anp32e |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546623/ https://www.ncbi.nlm.nih.gov/pubmed/33033242 http://dx.doi.org/10.1038/s41467-020-18821-x |
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