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A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs

Coronavirus disease 2019, known as COVID-19, is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The early, sensitive and specific detection of SARS-CoV-2 virus is widely recognized as the critical point in responding to the ongoing outbreak. Currently, the diagnosis is ba...

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Autores principales: Rocca, María Florencia, Zintgraff, Jonathan Cristian, Dattero, María Elena, Santos, Leonardo Silva, Ledesma, Martín, Vay, Carlos, Prieto, Mónica, Benedetti, Estefanía, Avaro, Martín, Russo, Mara, Nachtigall, Fabiane Manke, Baumeister, Elsa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546642/
https://www.ncbi.nlm.nih.gov/pubmed/33045283
http://dx.doi.org/10.1016/j.jviromet.2020.113991
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author Rocca, María Florencia
Zintgraff, Jonathan Cristian
Dattero, María Elena
Santos, Leonardo Silva
Ledesma, Martín
Vay, Carlos
Prieto, Mónica
Benedetti, Estefanía
Avaro, Martín
Russo, Mara
Nachtigall, Fabiane Manke
Baumeister, Elsa
author_facet Rocca, María Florencia
Zintgraff, Jonathan Cristian
Dattero, María Elena
Santos, Leonardo Silva
Ledesma, Martín
Vay, Carlos
Prieto, Mónica
Benedetti, Estefanía
Avaro, Martín
Russo, Mara
Nachtigall, Fabiane Manke
Baumeister, Elsa
author_sort Rocca, María Florencia
collection PubMed
description Coronavirus disease 2019, known as COVID-19, is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The early, sensitive and specific detection of SARS-CoV-2 virus is widely recognized as the critical point in responding to the ongoing outbreak. Currently, the diagnosis is based on molecular real time RT-PCR techniques, although their implementation is being threatened due to the extraordinary demand for supplies worldwide. That is why the development of alternative and / or complementary tests becomes so relevant. Here, we exploit the potential of mass spectrometry technology combined with machine learning algorithms, for the detection of COVID-19 positive and negative protein profiles directly from nasopharyngeal swabs samples. According to the preliminary results obtained, accuracy = 67.66 %, sensitivity = 61.76 %, specificity = 71.72 %, and although these parameters still need to be improved to be used as a screening technique, mass spectrometry-based methods coupled with multivariate analysis showed that it is an interesting tool that deserves to be explored as a complementary diagnostic approach due to the low cost and fast performance. However, further steps, such as the analysis of a large number of samples, should be taken in consideration to determine the applicability of the method developed.
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spelling pubmed-75466422020-10-13 A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs Rocca, María Florencia Zintgraff, Jonathan Cristian Dattero, María Elena Santos, Leonardo Silva Ledesma, Martín Vay, Carlos Prieto, Mónica Benedetti, Estefanía Avaro, Martín Russo, Mara Nachtigall, Fabiane Manke Baumeister, Elsa J Virol Methods Article Coronavirus disease 2019, known as COVID-19, is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The early, sensitive and specific detection of SARS-CoV-2 virus is widely recognized as the critical point in responding to the ongoing outbreak. Currently, the diagnosis is based on molecular real time RT-PCR techniques, although their implementation is being threatened due to the extraordinary demand for supplies worldwide. That is why the development of alternative and / or complementary tests becomes so relevant. Here, we exploit the potential of mass spectrometry technology combined with machine learning algorithms, for the detection of COVID-19 positive and negative protein profiles directly from nasopharyngeal swabs samples. According to the preliminary results obtained, accuracy = 67.66 %, sensitivity = 61.76 %, specificity = 71.72 %, and although these parameters still need to be improved to be used as a screening technique, mass spectrometry-based methods coupled with multivariate analysis showed that it is an interesting tool that deserves to be explored as a complementary diagnostic approach due to the low cost and fast performance. However, further steps, such as the analysis of a large number of samples, should be taken in consideration to determine the applicability of the method developed. Elsevier B.V. 2020-12 2020-10-09 /pmc/articles/PMC7546642/ /pubmed/33045283 http://dx.doi.org/10.1016/j.jviromet.2020.113991 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Rocca, María Florencia
Zintgraff, Jonathan Cristian
Dattero, María Elena
Santos, Leonardo Silva
Ledesma, Martín
Vay, Carlos
Prieto, Mónica
Benedetti, Estefanía
Avaro, Martín
Russo, Mara
Nachtigall, Fabiane Manke
Baumeister, Elsa
A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs
title A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs
title_full A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs
title_fullStr A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs
title_full_unstemmed A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs
title_short A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs
title_sort combined approach of maldi-tof mass spectrometry and multivariate analysis as a potential tool for the detection of sars-cov-2 virus in nasopharyngeal swabs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546642/
https://www.ncbi.nlm.nih.gov/pubmed/33045283
http://dx.doi.org/10.1016/j.jviromet.2020.113991
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