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The CD133(+) Stem/Progenitor-Like Cell Subset Is Increased in Human Milk and Peripheral Blood of HIV-Positive Women

Human milk is a significant source of different CD133(+) and/or CD34(+) stem/progenitor-like cell subsets in healthy women but their cell distribution and percentages in this compartment of HIV-positive women have not been explored. To date, a decrease of CD34(+) hematopoietic stem and progenitor ce...

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Autores principales: Valverde-Villegas, Jacqueline María, Naranjo-Gomez, Mar, Durand, Mélusine, Rutagwera, David, Bedin, Anne-Sophie, Kankasa, Chipepo, Debiesse, Ségolène, Nagot, Nicolas, Tuaillon, Edouard, Van de Perre, Philippe, Molès, Jean-Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546783/
https://www.ncbi.nlm.nih.gov/pubmed/33102251
http://dx.doi.org/10.3389/fcimb.2020.546189
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author Valverde-Villegas, Jacqueline María
Naranjo-Gomez, Mar
Durand, Mélusine
Rutagwera, David
Bedin, Anne-Sophie
Kankasa, Chipepo
Debiesse, Ségolène
Nagot, Nicolas
Tuaillon, Edouard
Van de Perre, Philippe
Molès, Jean-Pierre
author_facet Valverde-Villegas, Jacqueline María
Naranjo-Gomez, Mar
Durand, Mélusine
Rutagwera, David
Bedin, Anne-Sophie
Kankasa, Chipepo
Debiesse, Ségolène
Nagot, Nicolas
Tuaillon, Edouard
Van de Perre, Philippe
Molès, Jean-Pierre
author_sort Valverde-Villegas, Jacqueline María
collection PubMed
description Human milk is a significant source of different CD133(+) and/or CD34(+) stem/progenitor-like cell subsets in healthy women but their cell distribution and percentages in this compartment of HIV-positive women have not been explored. To date, a decrease of CD34(+) hematopoietic stem and progenitor cell frequencies in peripheral blood and bone marrow of HIV-positive patients has been reported. Herein, human milk and peripheral blood samples were collected between day 2–15 post-partum from HIV-positive and HIV-negative women, and cells were stained with stem cell markers and analyzed by flow cytometry. We report that the median percentage of CD45(+/high)CD34(−)CD133(+) cell subset from milk and blood was significantly higher in HIV-positive than in HIV-negative women. The percentage of CD45(dim)CD34(−)CD133(+) cell subset from blood was significantly higher in HIV-positive than HIV-negative women. Moreover, percentages of CD45(dim)CD34(+), CD45(dim)CD34(+)CD133(−), and CD45(+high)CD34(+)CD133(−) cell subsets from blood were significantly lower in HIV-positive than HIV-negative women. The CD133(+) stem/progenitor-like cell subsets are increased in early human milk and blood of HIV-positive women and are differentially distributed to CD34(+) cell subset frequencies which are decreased in blood.
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spelling pubmed-75467832020-10-22 The CD133(+) Stem/Progenitor-Like Cell Subset Is Increased in Human Milk and Peripheral Blood of HIV-Positive Women Valverde-Villegas, Jacqueline María Naranjo-Gomez, Mar Durand, Mélusine Rutagwera, David Bedin, Anne-Sophie Kankasa, Chipepo Debiesse, Ségolène Nagot, Nicolas Tuaillon, Edouard Van de Perre, Philippe Molès, Jean-Pierre Front Cell Infect Microbiol Cellular and Infection Microbiology Human milk is a significant source of different CD133(+) and/or CD34(+) stem/progenitor-like cell subsets in healthy women but their cell distribution and percentages in this compartment of HIV-positive women have not been explored. To date, a decrease of CD34(+) hematopoietic stem and progenitor cell frequencies in peripheral blood and bone marrow of HIV-positive patients has been reported. Herein, human milk and peripheral blood samples were collected between day 2–15 post-partum from HIV-positive and HIV-negative women, and cells were stained with stem cell markers and analyzed by flow cytometry. We report that the median percentage of CD45(+/high)CD34(−)CD133(+) cell subset from milk and blood was significantly higher in HIV-positive than in HIV-negative women. The percentage of CD45(dim)CD34(−)CD133(+) cell subset from blood was significantly higher in HIV-positive than HIV-negative women. Moreover, percentages of CD45(dim)CD34(+), CD45(dim)CD34(+)CD133(−), and CD45(+high)CD34(+)CD133(−) cell subsets from blood were significantly lower in HIV-positive than HIV-negative women. The CD133(+) stem/progenitor-like cell subsets are increased in early human milk and blood of HIV-positive women and are differentially distributed to CD34(+) cell subset frequencies which are decreased in blood. Frontiers Media S.A. 2020-09-24 /pmc/articles/PMC7546783/ /pubmed/33102251 http://dx.doi.org/10.3389/fcimb.2020.546189 Text en Copyright © 2020 Valverde-Villegas, Naranjo-Gomez, Durand, Rutagwera, Bedin, Kankasa, Debiesse, Nagot, Tuaillon, Van de Perre and Molès. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Valverde-Villegas, Jacqueline María
Naranjo-Gomez, Mar
Durand, Mélusine
Rutagwera, David
Bedin, Anne-Sophie
Kankasa, Chipepo
Debiesse, Ségolène
Nagot, Nicolas
Tuaillon, Edouard
Van de Perre, Philippe
Molès, Jean-Pierre
The CD133(+) Stem/Progenitor-Like Cell Subset Is Increased in Human Milk and Peripheral Blood of HIV-Positive Women
title The CD133(+) Stem/Progenitor-Like Cell Subset Is Increased in Human Milk and Peripheral Blood of HIV-Positive Women
title_full The CD133(+) Stem/Progenitor-Like Cell Subset Is Increased in Human Milk and Peripheral Blood of HIV-Positive Women
title_fullStr The CD133(+) Stem/Progenitor-Like Cell Subset Is Increased in Human Milk and Peripheral Blood of HIV-Positive Women
title_full_unstemmed The CD133(+) Stem/Progenitor-Like Cell Subset Is Increased in Human Milk and Peripheral Blood of HIV-Positive Women
title_short The CD133(+) Stem/Progenitor-Like Cell Subset Is Increased in Human Milk and Peripheral Blood of HIV-Positive Women
title_sort cd133(+) stem/progenitor-like cell subset is increased in human milk and peripheral blood of hiv-positive women
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546783/
https://www.ncbi.nlm.nih.gov/pubmed/33102251
http://dx.doi.org/10.3389/fcimb.2020.546189
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