Cargando…

Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton's Jelly (WJ-MSCs)

Tuning stem cells microenvironment in vitro may influence their regenerative properties. In this study Wharton's Jelly-derived mesenchymal stem cells (WJ-MSCs) were encapsulated in 3D hydrogels derived from human fibrin (FB) or platelet lysate (PL) and the oxygen level was adjusted to physiolog...

Descripción completa

Detalles Bibliográficos
Autores principales: Lech, Wioletta, Sarnowska, Anna, Kuczynska, Zuzanna, Dabrowski, Filip, Figiel-Dabrowska, Anna, Domanska-Janik, Krystyna, Buzanska, Leonora, Zychowicz, Marzena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547118/
https://www.ncbi.nlm.nih.gov/pubmed/33037314
http://dx.doi.org/10.1038/s41598-020-74066-0
_version_ 1783592366019444736
author Lech, Wioletta
Sarnowska, Anna
Kuczynska, Zuzanna
Dabrowski, Filip
Figiel-Dabrowska, Anna
Domanska-Janik, Krystyna
Buzanska, Leonora
Zychowicz, Marzena
author_facet Lech, Wioletta
Sarnowska, Anna
Kuczynska, Zuzanna
Dabrowski, Filip
Figiel-Dabrowska, Anna
Domanska-Janik, Krystyna
Buzanska, Leonora
Zychowicz, Marzena
author_sort Lech, Wioletta
collection PubMed
description Tuning stem cells microenvironment in vitro may influence their regenerative properties. In this study Wharton's Jelly-derived mesenchymal stem cells (WJ-MSCs) were encapsulated in 3D hydrogels derived from human fibrin (FB) or platelet lysate (PL) and the oxygen level was adjusted to physiological normoxia (5% O(2)). The influence of the type of the scaffold and physiological normoxia conditions was tested on the WJ-MSCs' survivability, proliferation, migratory potential, the level of expression of selected trophic factors, cytokines, and neural markers. Encapsulated WJ-MSCs revealed high survivability, stable proliferation rate, and ability to migrate out of the hydrogel and the up-regulated expression of all tested factors, as well as the increased expression of neural differentiation markers. Physiological normoxia stimulated proliferation of encapsulated WJ-MSCs and significantly enhanced their neuronal, but not glial, differentiation. Ex vivo studies with indirect co-culture of organotypic hippocampal slices and cell-hydrogel bio-constructs revealed strong neuroprotective effect of WJ-MSCs against neuronal death in the CA1 region of the rat hippocampus. This effect was potentiated further by FB scaffolds under 5% O(2) conditions. Our results indicating significant effect of oxygen and 3D cytoarchitecture suggest the urgent need for further optimization of the microenvironmental conditions to improve therapeutical competence of the WJ-MSCs population.
format Online
Article
Text
id pubmed-7547118
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-75471182020-10-14 Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton's Jelly (WJ-MSCs) Lech, Wioletta Sarnowska, Anna Kuczynska, Zuzanna Dabrowski, Filip Figiel-Dabrowska, Anna Domanska-Janik, Krystyna Buzanska, Leonora Zychowicz, Marzena Sci Rep Article Tuning stem cells microenvironment in vitro may influence their regenerative properties. In this study Wharton's Jelly-derived mesenchymal stem cells (WJ-MSCs) were encapsulated in 3D hydrogels derived from human fibrin (FB) or platelet lysate (PL) and the oxygen level was adjusted to physiological normoxia (5% O(2)). The influence of the type of the scaffold and physiological normoxia conditions was tested on the WJ-MSCs' survivability, proliferation, migratory potential, the level of expression of selected trophic factors, cytokines, and neural markers. Encapsulated WJ-MSCs revealed high survivability, stable proliferation rate, and ability to migrate out of the hydrogel and the up-regulated expression of all tested factors, as well as the increased expression of neural differentiation markers. Physiological normoxia stimulated proliferation of encapsulated WJ-MSCs and significantly enhanced their neuronal, but not glial, differentiation. Ex vivo studies with indirect co-culture of organotypic hippocampal slices and cell-hydrogel bio-constructs revealed strong neuroprotective effect of WJ-MSCs against neuronal death in the CA1 region of the rat hippocampus. This effect was potentiated further by FB scaffolds under 5% O(2) conditions. Our results indicating significant effect of oxygen and 3D cytoarchitecture suggest the urgent need for further optimization of the microenvironmental conditions to improve therapeutical competence of the WJ-MSCs population. Nature Publishing Group UK 2020-10-09 /pmc/articles/PMC7547118/ /pubmed/33037314 http://dx.doi.org/10.1038/s41598-020-74066-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lech, Wioletta
Sarnowska, Anna
Kuczynska, Zuzanna
Dabrowski, Filip
Figiel-Dabrowska, Anna
Domanska-Janik, Krystyna
Buzanska, Leonora
Zychowicz, Marzena
Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton's Jelly (WJ-MSCs)
title Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton's Jelly (WJ-MSCs)
title_full Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton's Jelly (WJ-MSCs)
title_fullStr Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton's Jelly (WJ-MSCs)
title_full_unstemmed Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton's Jelly (WJ-MSCs)
title_short Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton's Jelly (WJ-MSCs)
title_sort biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from wharton's jelly (wj-mscs)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547118/
https://www.ncbi.nlm.nih.gov/pubmed/33037314
http://dx.doi.org/10.1038/s41598-020-74066-0
work_keys_str_mv AT lechwioletta biomimeticmicroenvironmentalpreconditioningenhanceneuroprotectivepropertiesofhumanmesenchymalstemcellsderivedfromwhartonsjellywjmscs
AT sarnowskaanna biomimeticmicroenvironmentalpreconditioningenhanceneuroprotectivepropertiesofhumanmesenchymalstemcellsderivedfromwhartonsjellywjmscs
AT kuczynskazuzanna biomimeticmicroenvironmentalpreconditioningenhanceneuroprotectivepropertiesofhumanmesenchymalstemcellsderivedfromwhartonsjellywjmscs
AT dabrowskifilip biomimeticmicroenvironmentalpreconditioningenhanceneuroprotectivepropertiesofhumanmesenchymalstemcellsderivedfromwhartonsjellywjmscs
AT figieldabrowskaanna biomimeticmicroenvironmentalpreconditioningenhanceneuroprotectivepropertiesofhumanmesenchymalstemcellsderivedfromwhartonsjellywjmscs
AT domanskajanikkrystyna biomimeticmicroenvironmentalpreconditioningenhanceneuroprotectivepropertiesofhumanmesenchymalstemcellsderivedfromwhartonsjellywjmscs
AT buzanskaleonora biomimeticmicroenvironmentalpreconditioningenhanceneuroprotectivepropertiesofhumanmesenchymalstemcellsderivedfromwhartonsjellywjmscs
AT zychowiczmarzena biomimeticmicroenvironmentalpreconditioningenhanceneuroprotectivepropertiesofhumanmesenchymalstemcellsderivedfromwhartonsjellywjmscs