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A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients

OBJECTIVES: To examine whether specific T-cell-responses to SARS-CoV-2 peptides can be detected in COVID-19 using a whole-blood experimental setting, which may be further explored as a potential diagnostic tool. METHODS: We evaluated interferon (IFN)-γ levels after stimulating whole-blood with spike...

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Autores principales: Petrone, Linda, Petruccioli, Elisa, Vanini, Valentina, Cuzzi, Gilda, Najafi Fard, Saeid, Alonzi, Tonino, Castilletti, Concetta, Palmieri, Fabrizio, Gualano, Gina, Vittozzi, Pietro, Nicastri, Emanuele, Lepore, Luciana, Antinori, Andrea, Vergori, Alessandra, Caccamo, Nadia, Cantini, Fabrizio, Girardi, Enrico, Ippolito, Giuseppe, Grifoni, Alba, Goletti, Delia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547312/
https://www.ncbi.nlm.nih.gov/pubmed/33045370
http://dx.doi.org/10.1016/j.cmi.2020.09.051
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author Petrone, Linda
Petruccioli, Elisa
Vanini, Valentina
Cuzzi, Gilda
Najafi Fard, Saeid
Alonzi, Tonino
Castilletti, Concetta
Palmieri, Fabrizio
Gualano, Gina
Vittozzi, Pietro
Nicastri, Emanuele
Lepore, Luciana
Antinori, Andrea
Vergori, Alessandra
Caccamo, Nadia
Cantini, Fabrizio
Girardi, Enrico
Ippolito, Giuseppe
Grifoni, Alba
Goletti, Delia
author_facet Petrone, Linda
Petruccioli, Elisa
Vanini, Valentina
Cuzzi, Gilda
Najafi Fard, Saeid
Alonzi, Tonino
Castilletti, Concetta
Palmieri, Fabrizio
Gualano, Gina
Vittozzi, Pietro
Nicastri, Emanuele
Lepore, Luciana
Antinori, Andrea
Vergori, Alessandra
Caccamo, Nadia
Cantini, Fabrizio
Girardi, Enrico
Ippolito, Giuseppe
Grifoni, Alba
Goletti, Delia
author_sort Petrone, Linda
collection PubMed
description OBJECTIVES: To examine whether specific T-cell-responses to SARS-CoV-2 peptides can be detected in COVID-19 using a whole-blood experimental setting, which may be further explored as a potential diagnostic tool. METHODS: We evaluated interferon (IFN)-γ levels after stimulating whole-blood with spike and remainder-antigens peptides megapools (MP) derived from SARS-CoV-2 sequences; interleukin (IL)-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, eotaxin, basic fibroblast growth factor (FGF), granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-γ, Interferon gamma-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α, MIP-1β, Platelet-derived growth factor (PDGF), RANTES (regulated on activation, normal T cell expressed and secreted), tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF) were also evaluated. RESULTS: IFN-γ-response to spike and remainder-antigens MPs was significantly increased in 35 COVID-19 patients compared with 29 ‘no COVID-19’ individuals (medians spike-MP: 0.26 vs 0, p = 0.0002; medians remainder-antigens-MP: 0.07 vs 0.02; p = 0.02). This response was detected independently of patients' clinical parameters. IFN-γ-response to SARS-CoV-2-unrelated antigens cytomegalovirus (CMV) and Staphylococcal Enterotoxin B (SEB) was similar in COVID-19 compared with ‘no COVID-19’ individuals (median CMV: 3.46 vs 5.28, p = 0.16; median SEB: 12.68 vs 15.05; p = 0.1). In response to spike-MPs in COVID-19- compared with ‘no COVID-19’ -individuals, we found significant higher median of IL-2 (50.08 vs 0, p = 0.0018), IFN-γ (90.16 vs 0, p = 0.01), IL-4 (0.52 vs 0, p = 0.03), IL-13 (0.84 vs 0, p = 0.007) and MCP-1 (4602 vs 359.2, p = 0.05). CONCLUSIONS: Immune response to SARS-CoV-2 peptides in a whole-blood assay is associated with COVID-19 and it is characterized by both Th1 and Th2 profile. This experimental approach may be useful for developing new T-cell based diagnostic tests for disease and vaccine settings.
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spelling pubmed-75473122020-10-13 A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients Petrone, Linda Petruccioli, Elisa Vanini, Valentina Cuzzi, Gilda Najafi Fard, Saeid Alonzi, Tonino Castilletti, Concetta Palmieri, Fabrizio Gualano, Gina Vittozzi, Pietro Nicastri, Emanuele Lepore, Luciana Antinori, Andrea Vergori, Alessandra Caccamo, Nadia Cantini, Fabrizio Girardi, Enrico Ippolito, Giuseppe Grifoni, Alba Goletti, Delia Clin Microbiol Infect Original Article OBJECTIVES: To examine whether specific T-cell-responses to SARS-CoV-2 peptides can be detected in COVID-19 using a whole-blood experimental setting, which may be further explored as a potential diagnostic tool. METHODS: We evaluated interferon (IFN)-γ levels after stimulating whole-blood with spike and remainder-antigens peptides megapools (MP) derived from SARS-CoV-2 sequences; interleukin (IL)-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, eotaxin, basic fibroblast growth factor (FGF), granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-γ, Interferon gamma-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α, MIP-1β, Platelet-derived growth factor (PDGF), RANTES (regulated on activation, normal T cell expressed and secreted), tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF) were also evaluated. RESULTS: IFN-γ-response to spike and remainder-antigens MPs was significantly increased in 35 COVID-19 patients compared with 29 ‘no COVID-19’ individuals (medians spike-MP: 0.26 vs 0, p = 0.0002; medians remainder-antigens-MP: 0.07 vs 0.02; p = 0.02). This response was detected independently of patients' clinical parameters. IFN-γ-response to SARS-CoV-2-unrelated antigens cytomegalovirus (CMV) and Staphylococcal Enterotoxin B (SEB) was similar in COVID-19 compared with ‘no COVID-19’ individuals (median CMV: 3.46 vs 5.28, p = 0.16; median SEB: 12.68 vs 15.05; p = 0.1). In response to spike-MPs in COVID-19- compared with ‘no COVID-19’ -individuals, we found significant higher median of IL-2 (50.08 vs 0, p = 0.0018), IFN-γ (90.16 vs 0, p = 0.01), IL-4 (0.52 vs 0, p = 0.03), IL-13 (0.84 vs 0, p = 0.007) and MCP-1 (4602 vs 359.2, p = 0.05). CONCLUSIONS: Immune response to SARS-CoV-2 peptides in a whole-blood assay is associated with COVID-19 and it is characterized by both Th1 and Th2 profile. This experimental approach may be useful for developing new T-cell based diagnostic tests for disease and vaccine settings. Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases. 2021-02 2020-10-10 /pmc/articles/PMC7547312/ /pubmed/33045370 http://dx.doi.org/10.1016/j.cmi.2020.09.051 Text en © 2020 Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Original Article
Petrone, Linda
Petruccioli, Elisa
Vanini, Valentina
Cuzzi, Gilda
Najafi Fard, Saeid
Alonzi, Tonino
Castilletti, Concetta
Palmieri, Fabrizio
Gualano, Gina
Vittozzi, Pietro
Nicastri, Emanuele
Lepore, Luciana
Antinori, Andrea
Vergori, Alessandra
Caccamo, Nadia
Cantini, Fabrizio
Girardi, Enrico
Ippolito, Giuseppe
Grifoni, Alba
Goletti, Delia
A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients
title A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients
title_full A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients
title_fullStr A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients
title_full_unstemmed A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients
title_short A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients
title_sort whole blood test to measure sars-cov-2-specific response in covid-19 patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547312/
https://www.ncbi.nlm.nih.gov/pubmed/33045370
http://dx.doi.org/10.1016/j.cmi.2020.09.051
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